Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	4,457,932	IS5 (–) +4 bp	5.6%	intergenic (‑67/‑24)	yjgA ← / → pmbA	DUF615 domain‑containing protein YjgA/metalloprotease PmbA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	7 (0.080)	5/176	NT	7.4%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	= 4457935	84 (0.990)					intergenic (‑70/‑24)	yjgA/pmbA	DUF615 domain‑containing protein YjgA/metalloprotease PmbA
*	?	NC_000913	= 3653230	NA (NA)	3 (0.040)	3/168	NT	3.4%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	4457932 =	84 (0.990)					intergenic (‑67/‑27)	yjgA/pmbA	DUF615 domain‑containing protein YjgA/metalloprotease PmbA
Rejected: Frequency below/above cutoff threshold.

CATGATCTCATATCAGGGACTTGTTCGCACCTTCCttag‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3653196‑3653230 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCG  >  NC_000913/4457932‑4458033                                                                                                                                           CATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAG                                        <  1:238198/99‑1               AGGGACTTGTTCGCACCTTCCTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATT                          <  1:242935/99‑1                  GACTTGTTCGCACCTTCCTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTG                       <  1:1525035/99‑1                                      AGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGC   <  1:235426/99‑1                                      AGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGC   >  2:418893/1‑99                                      AGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGC   >  2:570469/1‑99                                       GACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCG  >  2:454216/1‑99                                                                                                                                           CATGATCTCATATCAGGGACTTGTTCGCACCTTCCttag‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3653196‑3653230 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCG  >  NC_000913/4457932‑4458033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.