breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsERR1473736_R1.good.fq3,692,707365,550,525100.0%99.0 bases99 bases99.8%
errorsERR1473736_R2.good.fq3,692,707365,550,525100.0%99.0 bases99 bases99.5%
total7,385,414731,101,050100.0%99.0 bases99 bases99.7%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,652157.21.9100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000003848
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500051
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.002

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.49791

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction0.1
Junction allow suboptimal matchesFALSE
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.05
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.2.6
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input13:58:23 25 Nov 202014:04:45 25 Nov 20206 minutes 22 seconds
Read alignment to reference genome14:04:45 25 Nov 202014:45:34 25 Nov 202040 minutes 49 seconds
Preprocessing alignments for candidate junction identification14:45:34 25 Nov 202014:51:28 25 Nov 20205 minutes 54 seconds
Preliminary analysis of coverage distribution14:51:28 25 Nov 202015:01:28 25 Nov 202010 minutes 0 seconds
Identifying junction candidates15:01:28 25 Nov 202015:01:29 25 Nov 20201 second
Re-alignment to junction candidates15:01:29 25 Nov 202015:11:49 25 Nov 202010 minutes 20 seconds
Resolving best read alignments15:11:49 25 Nov 202015:19:16 25 Nov 20207 minutes 27 seconds
Creating BAM files15:19:16 25 Nov 202015:28:01 25 Nov 20208 minutes 45 seconds
Tabulating error counts15:28:01 25 Nov 202015:31:30 25 Nov 20203 minutes 29 seconds
Re-calibrating base error rates15:31:30 25 Nov 202015:31:34 25 Nov 20204 seconds
Examining read alignment evidence15:31:34 25 Nov 202016:37:43 25 Nov 20201 hour 6 minutes 9 seconds
Polymorphism statistics16:37:43 25 Nov 202016:37:44 25 Nov 20201 second
Output16:37:44 25 Nov 202016:38:41 25 Nov 202057 seconds
Total 2 hours 40 minutes 18 seconds