Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	4,542,015	IS1 (+) +9 bp	13.9%	intergenic (+456/‑14)	fimB → / → fimE	regulator for fimA/regulator for fimA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3583428 =	NA (NA)	15 (0.160)	13/174	NT	14.9%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 4542023	77 (0.930)					intergenic (+464/‑14)	fimB/fimE	regulator for fimA/regulator for fimA
*	?	NC_000913	= 3584195	NA (NA)	10 (0.110)	10/176	NT	10.3%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	4542015 =	78 (0.930)					intergenic (+456/‑22)	fimB/fimE	regulator for fimA/regulator for fimA

GAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3583517‑3583428 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cCTAACTGAGATATATTTGAATGAATACCTATAGGAAACCTCAATCGGTCAAAATTAGCCCAATAGATAAAAAGATAACCACACACGGAGTGATGTGGC  <  NC_000913/4542023‑4541926                                                                                                                                                                                              GAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCTAACTGAG                                                                                           >  2:634974/1‑99      GGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCTAACTGAGATATA                                                                                      >  2:64201/1‑99                      GAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCTAACTGAGATATATTTGAATGAATACCTA                                                                      >  2:474351/1‑99                        AACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCTAACTGAGATATATTTGAATGAATACCTATA                                                                    <  2:1859138/99‑1                            GAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCTAACTGAGATATATTTGAATGAATACCTATAGGAA                                                                >  1:1599111/1‑99                            GAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCTAACTGAGATATATTTGAATGAATACCTATAGGAA                                                                >  1:1804352/1‑99                            GAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCTAACTGAGATATATTTGAATGAATACCTATAGGAA                                                                >  2:1356578/1‑99                                                 AAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCTAACTGAGATATATTTGAATGAATACCTATAGGAAACCTCAATCGGTCAAAATTAG                                           <  2:782113/99‑1                                                 AAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCTAACTGAGATATATTTGAATGAATACCTATAGGAAACCTCAATCGGTCAAAATTAG                                           >  2:2246940/1‑99                                                    CACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCTAACTGAGATATATTTGAATGAATACCTATAGGAAACCTCAATCGGTCAAAATTAGCCC                                        <  1:1531831/99‑1                                                     ACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCTAACTGAGATATATTTGAATGAATACCTATAGGAAACCTCAATCGGTCAAAATTAGCCCA                                       >  1:1056973/1‑99                                                              CACTAAATCAGTAAGTTGGCAGCATCACCCTAACTGAGATATATTTGAATGAATACCTATAGGAAACCTCAATCGGTCAAAATTAGCCCAATAGATAAA                              >  1:97705/1‑99                                                                     TCAGTAAGTTGGCAGCATCACCCTAACTGAGATATATTTGAATGAATACCTATAGGAAACCTCAATCGGTCAAAATTAGCCCAATAGATAAAAAGATAA                       <  1:2009893/99‑1                                                                            GTTGGCAGCATCACCCTAACTGAGATATATTTGAATGAATACCTATAGGAAACCTCAATCGGTCAAAATTAGCCCAATAGATAAAAAGATAACCACACA                <  1:2067024/99‑1                                                                                 CAGCATCACCCTAACTGAGATATATTTGAATGAATACCTATAGGAAACCTCAATCGGTCAAAATTAGCCCAATAGATAAAAAGATAACCACACACGGAG           >  1:1838992/1‑99                                                                                         CCCTAACTGAGATATATTTGAATGAATACCTATAGGAAACCTCAATCGGTCAAAATTAGCCCAATAGATAAAAAGATAACCACACACGGAGTGATGTGG   >  2:2351963/1‑99                                                                                          CCTAACTGAGATATATTTGAATGAATACCTATAGGAAACCTCAATCGGTCAAAATTAGCCCAATAGATAAAAAGATAACCACACACGGAGTGATGTGGC  <  2:1380620/99‑1                                                                                                                                                                                              GAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3583517‑3583428 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cCTAACTGAGATATATTTGAATGAATACCTATAGGAAACCTCAATCGGTCAAAATTAGCCCAATAGATAAAAAGATAACCACACACGGAGTGATGTGGC  <  NC_000913/4542023‑4541926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.