BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	366,290	C→T	93.8%	D6N (GAT→AAT)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,290	0	C	T	93.8%	4880.8 / 193.0	1346	D6N (GAT→AAT)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand): ref base C (36/47); new base T (593/670); total (629/717)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

Only 100 of 1348 total aligned reads displayed.

ACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGT  >  NC_000913/366192‑366388

   gcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGt                                                                                                 <  1:1326064‑M2/8‑1 (MQ=255)

      ccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAAt                                                                                              <  1:780473‑M2/11‑1 (MQ=255)

        tGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCa                                                                                            >  1:1586780/1‑99 (MQ=255)

        ggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCa                                                                                            <  1:228527‑M2/13‑1 (MQ=255)

        ggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCa                                                                                            <  1:734463‑M2/13‑1 (MQ=255)

        ggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCa                                                                                            <  2:1343495‑M2/13‑1 (MQ=255)

        ggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCa                                                                                            <  2:882576‑M2/13‑1 (MQ=255)

        ggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCa                                                                                            <  2:1100142‑M2/13‑1 (MQ=255)

          ggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCATg                                                                                          <  2:1807397‑M2/15‑1 (MQ=255)

          ggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCATg                                                                                          >  1:1290596‑M2/85‑99 (MQ=255)

           gccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAtgt                                                                                         <  1:881545‑M2/16‑2 (MQ=255)

            ccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCatgtc                                                                                        >  1:1000477‑M2/83‑97 (MQ=255)

             cagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATcatgtca                                                                                       <  1:204089‑M2/18‑4 (MQ=255)

             cagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATcatgtca                                                                                       <  1:618610‑M2/18‑4 (MQ=255)

             cagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATcatgtca                                                                                       <  2:1679290‑M2/18‑4 (MQ=255)

              agcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAAtcatgtcat                                                                                      >  2:342427‑M2/81‑95 (MQ=255)

               gcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATa                                                                                    >  2:1592535‑M2/80‑99 (MQ=255)

                cccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAg                                                                                   <  2:1786641‑M2/21‑1 (MQ=255)

                 ccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGc                                                                                  <  2:270016‑M2/22‑1 (MQ=255)

                  cgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCt                                                                                 >  1:1163802‑M2/77‑99 (MQ=255)

                   gagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTg                                                                                <  1:382732‑M2/24‑1 (MQ=255)

                    agcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGt                                                                               <  1:1681225‑M2/25‑1 (MQ=255)

                     gcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGtt                                                                              <  1:1631944‑M2/26‑1 (MQ=255)

                      tgtgCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGtt                                                                              <  1:1828410/99‑1 (MQ=255)

                      cgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGttt                                                                             <  1:282339‑M2/27‑1 (MQ=255)

                        agttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTcc                                                                           >  2:765410‑M2/71‑99 (MQ=255)

                         gttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCt                                                                          <  1:838394‑M2/30‑1 (MQ=255)

                          ttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCtg                                                                         <  2:1311116‑M2/31‑1 (MQ=255)

                             cgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCtgtgt                                                                      <  1:1396525‑M2/34‑1 (MQ=255)

                               tgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGa                                                                    >  1:666165‑M2/64‑99 (MQ=255)

                                 ccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGaaa                                                                  <  1:1051077‑M2/38‑1 (MQ=255)

                                  cggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAAt                                                                 <  2:657342‑M2/39‑1 (MQ=255)

                                    gttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTg                                                               <  1:1452467‑M2/41‑1 (MQ=255)

                                     ttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGt                                                              >  2:851694‑M2/58‑99 (MQ=255)

                                     ttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGt                                                              >  1:1751732‑M2/58‑99 (MQ=255)

                                          aagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATcc                                                         >  1:1512107‑M2/53‑99 (MQ=255)

                                            gggTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCg                                                        >  1:1430050/1‑99 (MQ=255)

                                            gaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGc                                                       >  2:1840784‑M2/51‑99 (MQ=255)

                                             ggTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGc                                                       <  1:940752/99‑1 (MQ=255)

                                             aaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCt                                                      >  1:702777‑M2/50‑99 (MQ=255)

                                              aaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTc                                                     >  2:933116‑M2/49‑99 (MQ=255)

                                               aaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTca                                                    <  2:809127‑M2/52‑1 (MQ=255)

                                                aagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTcac                                                   >  1:1908278‑M2/47‑99 (MQ=255)

                                                aaCGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTca                                                    >  2:722728/1‑99 (MQ=255)

                                                    gaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAtt                                               <  2:593609‑M2/57‑1 (MQ=255)

                                                     cAGGGGTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAtt                                               <  2:630274/99‑1 (MQ=255)

                                                     aatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTc                                              >  2:1542781‑M2/42‑99 (MQ=255)

                                                     aatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTc                                              <  1:538255‑M2/58‑1 (MQ=255)

                                                      atgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTcc                                             <  1:912976‑M2/59‑1 (MQ=255)

                                                       gggTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTcc                                             >  1:1803425/1‑99 (MQ=255)

                                                         atgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcaca                                          <  2:1171649‑M2/62‑1 (MQ=255)

                                                          tgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcacac                                         >  1:1351430‑M2/37‑99 (MQ=255)

                                                            tagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACacaa                                       <  2:1381593‑M2/65‑1 (MQ=255)

                                                            tagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACacaa                                       >  1:420680‑M2/35‑99 (MQ=255)

                                                             agccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACacaac                                      >  1:805953‑M2/34‑99 (MQ=255)

                                                              gccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACacaaca                                     <  2:1504447‑M2/67‑1 (MQ=255)

                                                               ccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACAt                                    >  2:1911072‑M2/32‑99 (MQ=255)

                                                                cgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATa                                   <  2:538308‑M2/69‑1 (MQ=255)

                                                                cgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATa                                   <  1:254328‑M2/69‑1 (MQ=255)

                                                                  tcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACg                                 >  1:11148‑M2/29‑99 (MQ=255)

                                                                  tcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACg                                 >  2:1423574‑M2/29‑99 (MQ=255)

                                                                   caagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGa                                >  2:1452718‑M2/28‑99 (MQ=255)

                                                                   caagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGa                                <  2:687379‑M2/72‑1 (MQ=255)

                                                                    aagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAg                               <  1:986164‑M2/73‑1 (MQ=255)

                                                                    aagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAg                               >  1:1859861‑M2/27‑99 (MQ=255)

                                                                       ttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCg                            >  2:567701‑M2/24‑99 (MQ=255)

                                                                          tcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGaa                         >  1:347994‑M2/21‑99 (MQ=255)

                                                                          tcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGaa                         >  2:1042813‑M2/21‑99 (MQ=255)

                                                                           ttGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGaa                         >  1:1553236/1‑99 (MQ=255)

                                                                           cataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGATATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAg                        <  2:379097‑M2/80‑1 (MQ=255)

                                                                            ataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGc                       >  2:966925‑M2/19‑99 (MQ=255)

                                                                            ataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGc                       >  2:922530‑M2/19‑99 (MQ=255)

                                                                            ataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGc                       >  2:1723554‑M2/19‑99 (MQ=255)

                                                                               attggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATa                    >  2:188070‑M2/16‑99 (MQ=255)

                                                                               attggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATa                    <  1:1715544‑M2/84‑1 (MQ=255)

                                                                               attggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATa                    >  2:1076990‑M2/16‑99 (MQ=255)

                                                                                ttggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATaa                   <  1:312069‑M2/85‑1 (MQ=255)

                                                                                  ggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAg                 >  2:1752855‑M2/13‑99 (MQ=255)

                                                                                    taccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAgtg               <  1:492790‑M2/89‑1 (MQ=255)

                                                                                    taccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAgtg               <  1:1783444‑M2/89‑1 (MQ=255)

                                                                                    taccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAgtg               >  2:474414‑M2/11‑99 (MQ=255)

                                                                                     accgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAgtgt              <  2:1265800‑M2/90‑1 (MQ=255)

                                                                                     accgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAgtgt              >  1:1084063‑M2/10‑99 (MQ=255)

                                                                                     accgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAgtgt              >  2:1487397‑M2/10‑99 (MQ=255)

                                                                                     accgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAgtgt              <  2:1018228‑M2/90‑1 (MQ=255)

                                                                                      ccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTa             <  2:507300‑M2/91‑1 (MQ=255)

                                                                                       cgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTaa            >  2:1232619‑M2/8‑99 (MQ=255)

                                                                                        gagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTaaa           >  1:1132941‑M2/7‑99 (MQ=255)

                                                                                         agctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAg          >  2:313462‑M2/6‑99 (MQ=255)

                                                                                           ctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGcc        <  2:1629626‑M2/96‑1 (MQ=255)

                                                                                           ctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGcc        >  1:460398‑M2/4‑99 (MQ=255)

                                                                                           ctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGcc        <  2:1207389‑M2/96‑1 (MQ=255)

                                                                                           ctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGcc        <  1:1269344‑M2/96‑1 (MQ=255)

                                                                                           ctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGcc        >  1:1208320‑M2/4‑99 (MQ=255)

                                                                                           ctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGcc        <  1:754845‑M2/96‑1 (MQ=255)

                                                                                            tcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCt       >  1:1482907‑M2/3‑99 (MQ=255)

                                                                                              gAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTgg     <  2:22560/99‑1 (MQ=255)

                                                                                              gAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTgg     <  1:592203/99‑1 (MQ=255)

                                                                                               aaTTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTggg    >  2:428867/1‑99 (MQ=255)

                                                                                               aaTTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTggg    >  2:1143976/1‑99 (MQ=255)

ACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGT  >  NC_000913/366192‑366388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`