Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,535,435 A→C 100% V145G (GTA→GGA)  envZ ← sensory histidine kinase EnvZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,535,4350AC100.0% 255.4 / NA 67V145G (GTA→GGA) envZsensory histidine kinase EnvZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (38/29);  total (38/29)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTG  >  NC_000913/3535341‑3535529
                                                                                              |                                                                                              
ccACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCa                                                                                            >  2:782345/1‑99 (MQ=255)
ccACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCa                                                                                            <  1:547238/99‑1 (MQ=255)
  aCGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGa                                                                                          <  1:918794/99‑1 (MQ=255)
  aCGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGa                                                                                          >  1:504081/1‑99 (MQ=255)
    gCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGata                                                                                        <  1:1411979/99‑1 (MQ=255)
    gCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGata                                                                                        >  2:1622053/1‑99 (MQ=255)
     ccccGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGatat                                                                                       >  2:599563/1‑99 (MQ=255)
     ccccGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGatat                                                                                       <  2:1700725/99‑1 (MQ=255)
      cccGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATAtt                                                                                      <  1:239730/99‑1 (MQ=255)
        cGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTgg                                                                                    <  2:1154817/99‑1 (MQ=255)
          ccTATCGCCAATAGCATAATCGCCAGCGTATAGCGGTACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGc                                                                                  >  1:195849/1‑99 (MQ=255)
            tATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGa                                                                                >  2:981658/1‑99 (MQ=255)
            tATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGa                                                                                >  2:615943/1‑99 (MQ=255)
            tATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGa                                                                                >  1:754062/1‑99 (MQ=255)
                gCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGc                                                                            <  2:1347901/99‑1 (MQ=255)
                gCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGc                                                                            <  2:102789/99‑1 (MQ=255)
                 ccAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGcc                                                                           <  2:186152/99‑1 (MQ=255)
                   aaTAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAg                                                                         >  1:1148773/1‑99 (MQ=255)
                   aaTAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAg                                                                         <  2:1914171/99‑1 (MQ=255)
                    aTAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAgg                                                                        <  1:1329937/99‑1 (MQ=255)
                     tAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGt                                                                       <  2:51283/99‑1 (MQ=255)
                       gCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGttt                                                                     <  1:961930/99‑1 (MQ=255)
                       gCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCGGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGttt                                                                     >  2:632730/1‑99 (MQ=255)
                          tAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCa                                                                  >  1:961507/1‑99 (MQ=255)
                                ccAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAga                                                            >  1:1528619/1‑99 (MQ=255)
                                 cAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAgac                                                           <  2:1441575/99‑1 (MQ=255)
                                 cAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAgac                                                           <  2:63664/99‑1 (MQ=255)
                                  aGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAgacg                                                          >  2:1013910/1‑99 (MQ=255)
                                  aGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAgacg                                                          >  2:113011/1‑99 (MQ=255)
                                   gCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAgacga                                                         >  1:470711/1‑99 (MQ=255)
                                      tataGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAg                                                      >  2:505559/1‑99 (MQ=255)
                                      tataGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAg                                                      >  1:185176/1‑99 (MQ=255)
                                      tataGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAg                                                      >  2:1057737/1‑99 (MQ=255)
                                       ataGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAgg                                                     <  2:838376/99‑1 (MQ=255)
                                        taGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGc                                                    >  2:884207/1‑99 (MQ=255)
                                           cGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGaa                                                 <  2:59568/99‑1 (MQ=255)
                                            ggAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAAc                                                <  2:1700921/99‑1 (MQ=255)
                                            ggAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAAc                                                >  2:1649733/1‑99 (MQ=255)
                                             gAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACt                                               >  1:1485240/1‑99 (MQ=255)
                                             gAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACt                                               >  1:1563116/1‑99 (MQ=255)
                                              aaCAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACtt                                              <  1:72444/99‑1 (MQ=255)
                                               aCAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACttt                                             >  2:1344288/1‑99 (MQ=255)
                                                  gCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTttgt                                          >  2:1306844/1‑99 (MQ=255)
                                                    ggAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTttgttg                                        <  1:1799952/99‑1 (MQ=255)
                                                     gagagaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGa                                       >  1:1649881/1‑99 (MQ=255)
                                                      agagaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGAc                                      <  2:830599/99‑1 (MQ=255)
                                                          aaaTCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCa                                  >  2:620909/1‑99 (MQ=255)
                                                          aaaTCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCa                                  >  2:399446/1‑99 (MQ=255)
                                                           aaTCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCaa                                 <  1:174889/99‑1 (MQ=255)
                                                                   tgatgaATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACt                         <  1:197166/99‑1 (MQ=255)
                                                                    gatgaATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACtt                        >  1:1745296/1‑99 (MQ=255)
                                                                     atgaATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTc                       <  2:625364/99‑1 (MQ=255)
                                                                     atgaATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTc                       <  1:1856368/99‑1 (MQ=255)
                                                                         aTTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTc                   >  2:483005/1‑99 (MQ=255)
                                                                         aTTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTc                   >  1:544517/1‑99 (MQ=255)
                                                                          tttCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCg                  >  1:202624/1‑99 (MQ=255)
                                                                           ttCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCgg                 >  2:118184/1‑99 (MQ=255)
                                                                             cGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGgc               <  1:484938/99‑1 (MQ=255)
                                                                              ggTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGgcc              >  2:440123/1‑99 (MQ=255)
                                                                               gTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTAAGCCAGACGACAGGCGAACTTTTGTTGACCGCAACGCGCACTTCCGTCAGgccg             >  2:632378/1‑99 (MQ=255)
                                                                                  aGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGccc          >  2:90362/1‑99 (MQ=255)
                                                                                      gCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGtt      >  2:914786/1‑99 (MQ=255)
                                                                                       cACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtg     >  2:221336/1‑99 (MQ=255)
                                                                                       cACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtg     <  2:1333134/99‑1 (MQ=255)
                                                                                        aCGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgc    <  1:1900043/99‑1 (MQ=255)
                                                                                         cgcgTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgct   >  1:158857/1‑99 (MQ=255)
                                                                                          gcgTCCCCAGATATTTGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgctg  <  1:1704133/99‑1 (MQ=255)
                                                                                              |                                                                                              
CCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTG  >  NC_000913/3535341‑3535529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑