| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,535,612 | A→C | 100% | I86S (ATC→AGC) | envZ ← | sensory histidine kinase EnvZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,535,612 | 0 | A | C | 100.0% | 202.6 / ‑6.7 | 55 | I86S (ATC→AGC) | envZ | sensory histidine kinase EnvZ |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (28/26); minor base G (1/0); total (29/26) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGC > NC_000913/3535518‑3535707 | gccCAGTTGCTGCGCCATTTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTGGTTGGAGTAGAGAGAGCTccc > 2:617449/1‑99 (MQ=255)gccCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTccc < 2:438604/99‑1 (MQ=255) cccAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTcccc > 1:684948/1‑99 (MQ=255) ccAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCa < 2:1785694/99‑1 (MQ=255) cAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAg > 2:170147/1‑99 (MQ=255) aGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGc < 2:1500023/99‑1 (MQ=255) gcgcCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGt > 2:770301/1‑99 (MQ=255) gcCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAg > 1:1627237/1‑99 (MQ=255) tCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGAtct < 1:885977/99‑1 (MQ=255) tCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGAtct < 2:1738068/99‑1 (MQ=255) tCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGAtct > 2:1354235/1‑99 (MQ=255) tGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTcc < 2:301934/99‑1 (MQ=255) gATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTccc < 2:468459/99‑1 (MQ=255) aTGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGCGTAGAGAGAGGTCCCCAGCTCACGGTAGGTCTcccc > 2:1739348/1‑98 (MQ=255) aTGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCg > 1:1749401/1‑99 (MQ=255) gCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACg > 1:1533890/1‑99 (MQ=255) ttAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGa > 2:71546/1‑99 (MQ=255) ttAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGa > 1:507306/1‑99 (MQ=255) tAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGaa < 2:1337086/99‑1 (MQ=255) tAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGaa > 1:976341/1‑99 (MQ=255) aaGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCGGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGaaa > 2:657378/1‑99 (MQ=255) aTTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCgg > 2:558248/1‑99 (MQ=255) tCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGa > 1:321579/1‑99 (MQ=255) tAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGc > 1:777029/1‑99 (MQ=255) tAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGc > 2:742410/1‑99 (MQ=255) tAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGc > 2:231606/1‑99 (MQ=255) tgtTGCGCCCAAAGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGcaca > 2:809253/1‑99 (MQ=255) ttGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaac < 1:845771/99‑1 (MQ=255) gcCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaaccaac < 1:1177347/99‑1 (MQ=255) ccAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTg < 1:1438279/99‑1 (MQ=255) aaCGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCg < 1:1766582/99‑1 (MQ=255) aCGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGt > 2:1735365/1‑99 (MQ=255) aCGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGt < 1:589382/99‑1 (MQ=255) gCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGc < 2:1513975/99‑1 (MQ=255) cAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGcc < 2:534122/99‑1 (MQ=255) gACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGt > 2:1015834/1‑99 (MQ=255) gACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGt > 1:703369/1‑99 (MQ=255) cTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcct < 1:797409/99‑1 (MQ=255) cTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcct < 1:1891700/99‑1 (MQ=255) ccTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCa > 2:1661831/1‑99 (MQ=255) tctTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGt < 2:1766610/99‑1 (MQ=255) cGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCa > 2:263986/1‑99 (MQ=255) ggCAGCCTCGTTGGAGTAGGGGGAGCTCCCAAGCTCACGGTAGAGCTCCCGACGGAAAGCGGGAGGCCCAACCAACTGCGTGCCGTCTTCCAGTTGCAg < 2:66334/99‑1 (MQ=255) ggCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAg > 1:1610622/1‑99 (MQ=255) ggCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAg < 2:633435/99‑1 (MQ=255) gCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGt > 1:156751/1‑99 (MQ=255) cAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGtt < 1:245108/99‑1 (MQ=255) gCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTg > 2:801931/1‑99 (MQ=255) ccTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGt < 2:1791652/99‑1 (MQ=255) ccTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGt < 2:1491116/99‑1 (MQ=255) cTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAATTGCGTGCCGTCCTCCAGTTGCAGTTTGTc < 2:793211/99‑1 (MQ=255) tCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCg < 2:352402/99‑1 (MQ=255) ttGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtc > 1:586293/1‑99 (MQ=255) gTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCaa < 2:1622894/99‑1 (MQ=255) gTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCaa > 2:360649/1‑99 (MQ=255) gagagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACAt > 2:1812255/1‑99 (MQ=255) agagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATa < 1:59633/99‑1 (MQ=255) agagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATa < 2:776089/99‑1 (MQ=255) agagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATa < 2:1864495/99‑1 (MQ=255) gagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATAc < 1:1213186/99‑1 (MQ=255) gagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGc > 2:1700982/1‑99 (MQ=255) | GCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGC > NC_000913/3535518‑3535707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |