Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,879,686	T→G	65.8%	Y145S (TAC→TCC)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,879,686	0	T	G	65.8%	103.4 / 99.2	88	Y145S (TAC→TCC)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand):  ref base T (14/16);  new base G (26/32);  total (40/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.64e-01

TGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACC  >  NC_000913/3879591‑3879780                                                                                                |                                                                                               tGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGgaga                                                                                             <  1:1562004/99‑1 (MQ=255) tGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGa                                                                                             <  2:1497993/99‑1 (MQ=255) tGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGa                                                                                             >  1:1545509/1‑99 (MQ=255)  gAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGAt                                                                                            <  2:2734265/99‑1 (MQ=255)   aaGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATc                                                                                           >  2:932152/1‑99 (MQ=255)   aaGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATc                                                                                           >  2:789937/1‑99 (MQ=255)     ggTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTg                                                                                         <  2:1923062/99‑1 (MQ=255)      gTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGa                                                                                        >  1:208292/1‑99 (MQ=255)         tCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACgg                                                                                     >  1:309641/1‑99 (MQ=255)            aGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGa                                                                                  <  2:1438678/99‑1 (MQ=255)             ggCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGaa                                                                                 >  1:1497717/1‑99 (MQ=255)             ggCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGaa                                                                                 >  1:1408843/1‑99 (MQ=255)             ggCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGaa                                                                                 >  1:1711414/1‑99 (MQ=255)              gCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAAt                                                                                <  2:1399885/99‑1 (MQ=255)               cTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAAtt                                                                               <  2:2771858/99‑1 (MQ=255)               cTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAAtt                                                                               <  1:1304935/99‑1 (MQ=255)                tGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAAttt                                                                              <  1:1137461/99‑1 (MQ=255)                tGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAAttt                                                                              >  2:862499/1‑99 (MQ=255)                  ggCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCTCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTa                                                                            <  2:1038682/99‑1 (MQ=255)                  ggCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTa                                                                            <  2:1737452/99‑1 (MQ=255)                     cAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTAccc                                                                         <  2:1850325/99‑1 (MQ=255)                     cAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTAccc                                                                         >  2:2330289/1‑99 (MQ=255)                     cAGAAACGCACCATGGTGCCGGTTTTTTCACTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGGGAATTTTAccc                                                                         <  2:2061745/99‑1 (MQ=255)                      aGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCt                                                                        >  1:2084212/1‑99 (MQ=255)                       gAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTc                                                                       >  2:897101/1‑99 (MQ=255)                         aaCGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTCCACCGTGTTCGGGGATCTGACGGTGAATTTTACCCTcgc                                                                     >  2:110913/1‑99 (MQ=255)                         aaCGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTcgc                                                                     >  1:2649752/1‑99 (MQ=255)                               cctcggTGCTGGTTTCTTCAGTCTGGCCGGTAACCGCCAGCGCGGCGTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCTCTGGa                                                               <  2:624474/95‑1 (MQ=255)                               ccATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGa                                                               <  2:2148791/99‑1 (MQ=255)                               ccATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGa                                                               <  2:2571803/99‑1 (MQ=255)                               ccATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGa                                                               >  2:142891/1‑99 (MQ=255)                                 aTGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATa                                                             >  1:2590314/1‑99 (MQ=255)                                  tGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATaa                                                            <  2:2031464/99‑1 (MQ=255)                                  tGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATaa                                                            <  2:137642/99‑1 (MQ=255)                                   ggTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAAc                                                           <  1:1240895/99‑1 (MQ=255)                                   ggTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAAc                                                           <  1:1431115/99‑1 (MQ=255)                                    gTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAAcc                                                          >  2:546199/1‑99 (MQ=255)                                     tGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCa                                                         <  1:1509962/99‑1 (MQ=255)                                     tGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCa                                                         <  2:355865/99‑1 (MQ=255)                                      gCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAg                                                        <  1:21467/99‑1 (MQ=255)                                       ccGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGc                                                       >  2:2662225/1‑99 (MQ=255)                                       ccGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGc                                                       <  2:875741/99‑1 (MQ=255)                                        cGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCt                                                      <  1:1542561/99‑1 (MQ=255)                                        cGGTTTTTTCAGTCGCGCCGGTACCGGCCAGCGGGGTCTGCGGTACACCGTGTTCGGAGATCTGCCGGTGAATTTTACCCTCGCGCTGGATAACCAGCt                                                      <  2:1932566/99‑1 (MQ=255)                                         ggTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTc                                                     >  2:1389230/1‑99 (MQ=255)                                          gTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTcc                                                    >  2:732211/1‑99 (MQ=255)                                           ttttttCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCa                                                   >  1:2019223/1‑99 (MQ=255)                                             ttttCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGt                                                 <  1:2708497/99‑1 (MQ=255)                                                  aGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTg                                            <  2:1744138/99‑1 (MQ=255)                                                  aGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTg                                            <  1:620982/99‑1 (MQ=255)                                                  aGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTg                                            <  1:435430/99‑1 (MQ=255)                                                    tctcGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCg                                          >  2:2806377/1‑99 (MQ=255)                                                           ggTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGc                                   <  1:1504360/99‑1 (MQ=255)                                                            gTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCg                                  >  1:1348514/1‑99 (MQ=255)                                                            gTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCg                                  >  1:2140131/1‑99 (MQ=255)                                                            gTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCg                                  >  2:894161/1‑99 (MQ=255)                                                             tAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGt                                 >  2:1366128/1‑99 (MQ=255)                                                               aCCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGttt                               >  1:1357965/1‑99 (MQ=255)                                                                ccGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTta                              >  2:282636/1‑99 (MQ=255)                                                                ccGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTta                              <  1:2631643/99‑1 (MQ=255)                                                                ccGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTta                              <  1:1559545/99‑1 (MQ=255)                                                                 cGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTtac                             <  2:208292/99‑1 (MQ=255)                                                                 cGCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTtac                             <  2:2642354/99‑1 (MQ=255)                                                                  gCCAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTtact                            <  1:2150654/99‑1 (MQ=255)                                                                   ccAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTtacta                           <  1:482079/99‑1 (MQ=255)                                                                   ccAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTtacta                           >  1:1962534/1‑99 (MQ=255)                                                                   ccAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTtacta                           >  1:2784401/1‑99 (MQ=255)                                                                    cAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTtactac                          <  2:1626778/99‑1 (MQ=255)                                                                    cAGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTtactac                          <  1:2698019/99‑1 (MQ=255)                                                                     aGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTAcc                         <  2:2795017/99‑1 (MQ=255)                                                                     aGCGGGGCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTAcc                         >  2:1285117/1‑99 (MQ=255)                                                                      gCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCg                        >  2:11447/1‑99 (MQ=255)                                                                         gggCCTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGaaa                     <  2:973454/99‑1 (MQ=255)                                                                            ccTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAacac                  <  1:494219/99‑1 (MQ=255)                                                                            ccTGCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAacac                  <  2:1181002/99‑1 (MQ=255)                                                                              tGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCa                >  1:2231503/1‑99 (MQ=255)                                                                               gCGGTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCaa               <  2:292155/99‑1 (MQ=255)                                                                                 ggTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACg             >  2:354175/1‑99 (MQ=255)                                                                                  gTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGc            >  2:891657/1‑99 (MQ=255)                                                                                  gTACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGc            >  1:707405/1‑99 (MQ=255)                                                                                   tACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGcc           >  1:437631/1‑99 (MQ=255)                                                                                   tACACCGTGTTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGcc           >  1:1997487/1‑99 (MQ=255)                                                                                      acCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTg        >  2:2242684/1‑99 (MQ=255)                                                                                         gtgtTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAg     <  2:1537351/99‑1 (MQ=255)                                                                                          ttttCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAATCAGTTTCTGTTTTTGCGAAAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGa    >  2:317394/3‑99 (MQ=255)                                                                                          tgtTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGa    <  1:1250006/99‑1 (MQ=255)                                                                                           gtTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGAc   <  2:2629717/99‑1 (MQ=255)                                                                                           gtTCGGAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGAc   <  1:2080623/99‑1 (MQ=255)                                                                                            ttCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGTGTTTACTACCGAAACACCAACGCCGTGCAGAcc  <  1:2362585/99‑1 (MQ=255)                                                                                                |                                                                                               TGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACC  >  NC_000913/3879591‑3879780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑