Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,725,799 Δ1 bp 100% intergenic (‑53/+270) kgtP ← / ← rrfG alpha‑ketoglutarate:H(+) symporter/5S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,725,7990T.99.0% 394.4 / ‑1.3 96intergenic (‑53/+270)kgtP/rrfGalpha‑ketoglutarate:H(+) symporter/5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base . (43/52);  total (43/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 110 total aligned reads displayed.

ACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAG  >  NC_000913/2725702‑2725891
                                                                                                 |                                                                                            
actactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTAtt                                                                                             <  2:352537/99‑1 (MQ=255)
 ctactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTAttt                                                                                            >  1:348692/1‑99 (MQ=255)
 ctactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTAttt                                                                                            <  1:40802/99‑1 (MQ=255)
 ctactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTAttt                                                                                            <  2:1688314/99‑1 (MQ=255)
  tactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTAtttt                                                                                           <  2:141211/99‑1 (MQ=255)
  tactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTAtttt                                                                                           <  1:1950902/99‑1 (MQ=255)
   actTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTAttttt                                                                                          >  1:101882/1‑99 (MQ=255)
      tGTCGGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTAtttttttg                                                                                       <  1:1235743/99‑2 (MQ=255)
      tGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTAtttttttg                                                                                       <  2:2260931/99‑2 (MQ=255)
       gTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTAtttttttgt                                                                                      <  1:1584076/99‑3 (MQ=255)
       gTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTAtttttttgt                                                                                      <  2:548165/99‑3 (MQ=255)
       gTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTAtttttttgt                                                                                      <  1:2018563/99‑3 (MQ=255)
          aGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTaaa                                                                                  <  1:1319025/99‑1 (MQ=255)
          aGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTaaa                                                                                  >  1:50202/1‑99 (MQ=255)
          aGTTTGCTGGCTGCCGTTACGGGACTTTCAGCCATGCCATTATGTCTCCTGCCGTAAGCCGATGCTTTTGTCGGTCGCTTTTGTTTAtttttttttaaa                                                                                   <  2:1720202/99‑5 (MQ=255)
                cTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAat                                                                            <  1:2516555/99‑1 (MQ=255)
                cTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAat                                                                            <  2:612469/99‑1 (MQ=255)
                 tGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAata                                                                           <  1:2431408/99‑1 (MQ=255)
                  gTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAatat                                                                          <  1:619670/99‑1 (MQ=255)
                  gTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAatat                                                                          >  1:1769127/1‑99 (MQ=255)
                   tCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTATAGGAACtatt                                                                         >  2:940691/1‑99 (MQ=255)
                   tCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAAtatt                                                                         >  1:525168/1‑99 (MQ=255)
                    cTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAAtgtta                                                                        >  1:1554531/1‑99 (MQ=255)
                    cTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAAtatta                                                                        <  2:380993/99‑1 (MQ=255)
                    cTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAAtatta                                                                        <  1:1730782/99‑1 (MQ=255)
                      gCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATtata                                                                      <  1:351785/99‑1 (MQ=255)
                        cGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACa                                                                    <  2:1531240/99‑1 (MQ=255)
                         gTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACAt                                                                   <  2:281078/99‑1 (MQ=255)
                          ttACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACAtt                                                                  >  1:2432146/1‑99 (MQ=255)
                          ttACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACAtt                                                                  >  1:1580565/1‑99 (MQ=255)
                          ttACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACAtt                                                                  >  2:2531757/1‑99 (MQ=255)
                          ttACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACAtt                                                                  <  2:2445787/99‑1 (MQ=255)
                           tACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACAttt                                                                 <  1:995020/99‑1 (MQ=255)
                           tACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACAttt                                                                 <  2:1560887/99‑1 (MQ=255)
                             cAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATttgt                                                               <  1:2314582/99‑1 (MQ=255)
                              aGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATttgtt                                                              <  1:471642/99‑1 (MQ=255)
                               gTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATttgttg                                                             >  1:213523/1‑99 (MQ=255)
                               gTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATttgttg                                                             >  2:1203388/1‑99 (MQ=255)
                               gTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATttgttg                                                             >  2:1368020/1‑99 (MQ=255)
                                tACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGc                                                            >  2:2459986/1‑99 (MQ=255)
                                tACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGc                                                            >  2:1213547/1‑99 (MQ=255)
                                tACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGc                                                            >  2:1092909/1‑99 (MQ=255)
                                 aCTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCa                                                           <  1:2629323/99‑1 (MQ=255)
                                   tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTT‑TAAAGGAAATATTATACATTTGTTGCata                                                         <  2:2696997/99‑1 (MQ=255)
                                   tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCata                                                         <  1:2342874/99‑1 (MQ=255)
                                    ttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCatat                                                        <  1:2267814/99‑1 (MQ=255)
                                     tCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATc                                                       <  1:581397/99‑1 (MQ=255)
                                     tCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATc                                                       >  2:1086372/1‑99 (MQ=255)
                                     tCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTAGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATc                                                       <  2:2547124/99‑1 (MQ=255)
                                        gccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCAtt                                                    <  2:110654/99‑1 (MQ=255)
                                        gccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCAtt                                                    >  1:540531/1‑99 (MQ=255)
                                         ccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTa                                                   <  1:2068286/99‑1 (MQ=255)
                                           atgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATg                                                 >  1:496541/1‑99 (MQ=255)
                                           atgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATg                                                 >  1:2574467/1‑99 (MQ=255)
                                            tgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGc                                                >  2:106560/1‑99 (MQ=255)
                                              ccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCaa                                              <  2:1200900/99‑1 (MQ=255)
                                                atTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAAcc                                            <  2:1493414/99‑1 (MQ=255)
                                                   aTGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTa                                         <  2:368887/99‑1 (MQ=255)
                                                     gTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAAc                                       <  1:2084813/99‑1 (MQ=255)
                                                     gTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAAc                                       <  2:66623/99‑1 (MQ=255)
                                                     gTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAAc                                       <  2:1549561/99‑1 (MQ=255)
                                                      tctcCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAAcc                                      <  2:118542/99‑1 (MQ=255)
                                                         ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATg                                   >  1:2425779/1‑99 (MQ=255)
                                                          cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGa                                  >  2:2722016/1‑99 (MQ=255)
                                                          cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGa                                  <  1:2464997/99‑1 (MQ=255)
                                                           tGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGaa                                 >  2:2137167/1‑99 (MQ=255)
                                                           tGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGaa                                 >  2:177055/1‑99 (MQ=255)
                                                           tGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGaa                                 <  2:1718480/99‑1 (MQ=255)
                                                           tGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGaa                                 <  2:614240/99‑1 (MQ=255)
                                                            gCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAAt                                <  2:212941/99‑1 (MQ=255)
                                                              cGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAAttt                              >  2:56553/1‑99 (MQ=255)
                                                                tAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttag                            <  2:2061174/99‑1 (MQ=255)
                                                                tAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttag                            <  1:2161733/99‑1 (MQ=255)
                                                                tAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttag                            >  1:2386859/1‑99 (MQ=255)
                                                                tAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttag                            >  2:88857/1‑99 (MQ=255)
                                                                 aaTCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttagt                           <  1:1870293/99‑1 (MQ=255)
                                                                 aaTCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttagt                           >  2:2380932/1‑99 (MQ=255)
                                                                  aTCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttagtt                          >  2:1804726/1‑99 (MQ=255)
                                                                     cGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGc                       <  2:2806022/99‑1 (MQ=255)
                                                                     cGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGc                       <  2:2558539/99‑1 (MQ=255)
                                                                      gATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCa                      >  2:1257804/1‑99 (MQ=255)
                                                                      gATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCa                      >  1:193365/1‑99 (MQ=255)
                                                                      gATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCa                      <  1:2793633/99‑1 (MQ=255)
                                                                       aTGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAg                     >  1:2775826/1‑99 (MQ=255)
                                                                       aTGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAg                     >  2:339650/1‑99 (MQ=255)
                                                                       aTGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAg                     >  1:2830626/1‑99 (MQ=255)
                                                                       aTGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAg                     >  2:902311/1‑99 (MQ=255)
                                                                        tGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAgg                    >  1:1967826/1‑99 (MQ=255)
                                                                          cTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGaa                  >  2:590771/1‑99 (MQ=255)
                                                                           ttttGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGaaa                 <  1:1046170/99‑1 (MQ=255)
                                                                            tttGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGaaaa                <  2:1380979/99‑1 (MQ=255)
                                                                             ttGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAAt               <  2:1193015/99‑1 (MQ=255)
                                                                              tGTCGGTCGCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATg              <  2:362623/99‑1 (MQ=255)
                                                                                tcggtcgCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGt            >  2:516218/1‑99 (MQ=255)
                                                                                tcggtcgCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGt            <  2:2751796/99‑1 (MQ=255)
                                                                                   gtcgCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATCCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTAt         <  2:1823210/99‑1 (MQ=255)
                                                                                   gtcgCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTAt         <  1:1606460/99‑1 (MQ=255)
                                                                                     cgCTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTg       <  1:119521/99‑1 (MQ=255)
                                                                                       cTTTTGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgag     >  2:1225190/1‑99 (MQ=255)
                                                                                          ttGTTTA‑TTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgaggag  >  1:533814/1‑99 (MQ=255)
                                                                                                 |                                                                                            
ACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAG  >  NC_000913/2725702‑2725891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑