Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	2,984,711	29 bp→TTTTTTTTTAATGATACGGCGACCACCGA	6.9%	coding (854‑882/1182 nt)	yqeF ←	putative acyltransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2984710	78 (0.810)	4 (0.060) +TTTTTTTTTAATGATACGGCGACCACCGA	4/118	NT	6.9%	coding (883/1182 nt)	yqeF	putative acyltransferase
	?	NC_000913	2984740 =	84 (0.870)					coding (853/1182 nt)	yqeF	putative acyltransferase

CCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2984671‑2984710 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GATCTACACCAACGCTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTT  >  NC_000913/2984740‑2984806                                         |||||||||||||||||||||||||||||                                                                    CCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGTATTTTTTTGAATAATACGGCGACCACCGAGATCTACACCAACGCTGGCAAATGCGCGAA                                       <  2:1695566/99‑1     CTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACCAACGCTGGCAAATGCGCGAATGCG                                   <  2:1756032/99‑1       GCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACCAACGCTGGCAAATGCGCGAATGCGGG                                 <  2:2111494/99‑1                                GCGGGTCGCATTTTTCTGCGTAATACGCCGCCCTCCGAGATCTACACCAACGCTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCG        <  2:1739986/99‑1                                      cttTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACCAACGCTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTT  <  2:1055798/96‑1                                         |||||||||||||||||||||||||||||                                                                    CCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2984671‑2984710 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GATCTACACCAACGCTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTT  >  NC_000913/2984740‑2984806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.