Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,535,612	A→C	I86S (ATC→AGC)	envZ ←	sensory histidine kinase EnvZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,535,612	0	A	C	100.0%	245.5 / NA	78	I86S (ATC→AGC)	envZ	sensory histidine kinase EnvZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (47/31);  total (47/31)

CCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGC  >  NC_000913/3535519‑3535707                                                                                              |                                                                                                cccAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTcccc                                                                                            >  1:279447/1‑99 (MQ=255)     gTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCt                                                                                        >  2:339660/1‑99 (MQ=255)         ctgcGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACg                                                                                    >  2:1649968/1‑99 (MQ=255)           gcgcCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGt                                                                                  >  2:1782203/1‑99 (MQ=255)            cgcCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTa                                                                                 <  1:541424/99‑1 (MQ=255)             gcCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAg                                                                                >  1:117953/1‑99 (MQ=255)               cATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGAt                                                                              >  1:2077226/1‑99 (MQ=255)                aTCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGAtc                                                                             >  1:2341779/1‑99 (MQ=255)                aTCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGAtc                                                                             <  2:224527/99‑1 (MQ=255)                 tCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGAtct                                                                            >  1:1378960/1‑99 (MQ=255)                  cTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGAtctc                                                                           >  2:1892781/1‑99 (MQ=255)                  cTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGAtctc                                                                           <  2:137714/99‑1 (MQ=255)                   tGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTcc                                                                          <  2:751185/99‑1 (MQ=255)                   tGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTcc                                                                          >  2:1263574/1‑99 (MQ=255)                   tGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTcc                                                                          >  1:2043147/1‑99 (MQ=255)                    gATGGCTTAAGAATTGATAGTGTTGCGCGCAACGCAGACCTGAATCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCTGCTCACGGTAGATCTccc                                                                         <  2:830643/99‑1 (MQ=255)                     aTGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCg                                                                        >  2:976807/1‑99 (MQ=255)                      tGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGa                                                                       >  1:1375632/1‑99 (MQ=255)                      tGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGa                                                                       <  1:370945/99‑1 (MQ=255)                      tGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCGCCCGa                                                                       >  2:825973/1‑99 (MQ=255)                       ggCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGAc                                                                      >  1:981557/1‑99 (MQ=255)                         cTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACgg                                                                    >  1:1064839/1‑99 (MQ=255)                         cTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACgg                                                                    >  1:2208729/1‑99 (MQ=255)                          ttAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGa                                                                   >  2:2155730/1‑99 (MQ=255)                            aaGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGaaa                                                                 >  1:2440124/1‑99 (MQ=255)                            aaGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGaaa                                                                 >  2:433695/1‑99 (MQ=255)                             aGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAg                                                                >  2:948221/1‑99 (MQ=255)                             aGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAg                                                                <  1:2037321/99‑1 (MQ=255)                             aGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAg                                                                >  1:1589091/1‑99 (MQ=255)                              gAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGc                                                               >  1:2481907/1‑99 (MQ=255)                               aaTTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCg                                                              >  2:1583406/1‑99 (MQ=255)                               aaTTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGGAGAGAGAGCTCCCCTGCTCACGGTAGATCTCCCGACGGAAAGCg                                                              >  2:129734/1‑99 (MQ=255)                                 ttCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCggg                                                            <  2:988149/99‑1 (MQ=255)                                 ttCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCggg                                                            >  1:1128022/1‑99 (MQ=255)                                 ttCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCggg                                                            >  2:2038503/1‑99 (MQ=255)                                     tAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGc                                                        >  1:587396/1‑99 (MQ=255)                                     tAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGc                                                        >  1:1227824/1‑99 (MQ=255)                                     tAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGCTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGc                                                        >  2:969926/1‑99 (MQ=255)                                       gtgtTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGcac                                                      >  2:1660926/1‑99 (MQ=255)                                          ttGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaac                                                   <  2:2454580/99‑1 (MQ=255)                                          ttGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaac                                                   >  2:2344222/1‑99 (MQ=255)                                          ttGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaac                                                   >  2:1763834/1‑99 (MQ=255)                                                ccAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTg                                             <  2:640990/99‑1 (MQ=255)                                                 cAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGc                                            >  1:221160/1‑99 (MQ=255)                                                  aaCGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCg                                           <  1:2103904/99‑1 (MQ=255)                                                   aCGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGt                                          <  1:407808/99‑1 (MQ=255)                                                     gCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGc                                        <  1:1046973/99‑1 (MQ=255)                                                          ccTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcc                                   <  1:2270647/99‑1 (MQ=255)                                                           cTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcct                                  <  1:910328/99‑1 (MQ=255)                                                            tGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcctc                                 >  1:776315/1‑99 (MQ=255)                                                            tGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcctc                                 <  1:980980/99‑1 (MQ=255)                                                               ctctTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAg                              <  1:1070474/99‑1 (MQ=255)                                                               ctctTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAg                              <  1:889920/99‑1 (MQ=255)                                                                tctTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGt                             <  1:1347999/99‑1 (MQ=255)                                                                   tCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGc                          >  1:1993953/1‑99 (MQ=255)                                                                     ggCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAg                        <  2:1763432/99‑1 (MQ=255)                                                                      gCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGt                       >  1:337349/1‑99 (MQ=255)                                                                         gCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTg                    >  2:1215129/1‑99 (MQ=255)                                                                         gCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTg                    >  1:684402/1‑99 (MQ=255)                                                                         gCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTg                    >  2:693656/1‑99 (MQ=255)                                                                         gCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTg                    >  2:1181208/1‑99 (MQ=255)                                                                          ccTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGt                   >  1:993714/1‑99 (MQ=255)                                                                           cTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTc                  <  2:904100/99‑1 (MQ=255)                                                                             cGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCgg                <  1:2124597/99‑1 (MQ=255)                                                                             cGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCgg                <  1:416142/99‑1 (MQ=255)                                                                               ttGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtc              >  2:1909251/1‑99 (MQ=255)                                                                               ttGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtc              >  2:1975784/1‑99 (MQ=255)                                                                               ttGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtc              >  1:1517790/1‑99 (MQ=255)                                                                               ttGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtc              >  1:443234/1‑99 (MQ=255)                                                                                tGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtca             <  1:1576299/99‑1 (MQ=255)                                                                                tGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtca             <  1:790841/99‑1 (MQ=255)                                                                                  gAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtcatc           <  1:1864474/99‑1 (MQ=255)                                                                                    gTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCaa         <  2:2268497/99‑1 (MQ=255)                                                                                    gTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCaa         <  1:1363185/99‑1 (MQ=255)                                                                                      agagagagCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACa       <  2:395498/99‑1 (MQ=255)                                                                                      agagagagCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACa       >  2:487792/1‑99 (MQ=255)                                                                                      agagagagCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACa       <  2:2026467/99‑1 (MQ=255)                                                                                      agagagagCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACa       <  2:1443813/99‑1 (MQ=255)                                                                                       gagagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAATCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACAt      >  2:310423/1‑99 (MQ=255)                                                                                        agagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATa     <  1:280561/99‑1 (MQ=255)                                                                                        agagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATa     <  2:1268021/99‑1 (MQ=255)                                                                                        agagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATa     <  1:1835057/99‑1 (MQ=255)                                                                                        agagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATa     <  2:1157193/99‑1 (MQ=255)                                                                                         gagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATAc    >  1:1784426/1‑99 (MQ=255)                                                                                         gagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATAc    >  2:1136740/1‑99 (MQ=255)                                                                                          agagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACg   >  2:718728/1‑99 (MQ=255)                                                                                           gagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGc  >  2:69690/1‑99 (MQ=255)                                                                                              |                                                                                                CCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGC  >  NC_000913/3535519‑3535707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑