BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	366,301	Δ1 bp	coding (5/3075 nt)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,300	0	G	.	99.4%	101950.4 / inf	25543	coding (6/3075 nt)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand): ref base G (73/88); new base . (13057/12321); total (13131/12412)
Fisher's exact test for biased strand distribution p-value = 1.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 26915 total aligned reads displayed.

GCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAG  >  NC_000913/366202‑366398

 gccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAtgt                                                                                                   <  2:660730‑M2/16‑2 (MQ=255)

 gccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAtgt                                                                                                   <  2:1458896‑M2/16‑2 (MQ=255)

  ccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCatgtc                                                                                                  <  1:2637834‑M2/17‑3 (MQ=255)

    agcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAAtcatgtcat                                                                                                >  1:752685‑M2/81‑95 (MQ=255)

    agcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAAtcatgtcat                                                                                                >  1:68736‑M2/81‑95 (MQ=255)

    agcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAAtcatgtcat                                                                                                >  2:2694101‑M2/81‑95 (MQ=255)

     gcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATa                                                                                              >  1:298899‑M2/80‑99 (MQ=255)

     gcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATa                                                                                              >  2:1881517‑M2/80‑99 (MQ=255)

     gcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATa                                                                                              >  1:1751695‑M2/80‑99 (MQ=255)

     gcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATa                                                                                              >  1:3797019‑M2/80‑99 (MQ=255)

       ccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGc                                                                                            <  2:862231‑M2/22‑1 (MQ=255)

        cgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCt                                                                                           >  1:3725351‑M2/77‑99 (MQ=255)

           gcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGtt                                                                                        <  1:351190‑M2/26‑1 (MQ=255)

           gcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGtt                                                                                        >  1:2370538‑M2/74‑99 (MQ=255)

              agttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTcc                                                                                     <  2:222195‑M2/29‑1 (MQ=255)

               gttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCt                                                                                    >  2:2018629‑M2/70‑99 (MQ=255)

                ttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCtg                                                                                   <  1:2437541‑M2/31‑1 (MQ=255)

                    gtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCtgtgtg                                                                               >  2:3037291‑M2/65‑99 (MQ=255)

                     tgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGa                                                                              >  1:1068151‑M2/64‑99 (MQ=255)

                       ccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGaaa                                                                            >  2:3528554‑M2/62‑99 (MQ=255)

                        cggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAAt                                                                           <  2:1099503‑M2/39‑1 (MQ=255)

                        cggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAAt                                                                           <  1:430719‑M2/39‑1 (MQ=255)

                        cggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAAt                                                                           <  2:3109646‑M2/39‑1 (MQ=255)

                         ggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAAtt                                                                          >  1:2091194‑M2/60‑99 (MQ=255)

                         ggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAAtt                                                                          <  1:202460‑M2/40‑1 (MQ=255)

                          gttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTg                                                                         <  2:2562881‑M2/41‑1 (MQ=255)

                          gttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTg                                                                         >  1:2777188‑M2/59‑99 (MQ=255)

                           ttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGt                                                                        >  2:1168‑M2/58‑99 (MQ=255)

                           ttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGt                                                                        <  2:2087925‑M2/42‑1 (MQ=255)

                            tgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGtt                                                                       <  2:92894‑M2/43‑1 (MQ=255)

                             gtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTa                                                                      >  1:3060933‑M2/56‑99 (MQ=255)

                              tgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTAt                                                                     <  1:888757‑M2/45‑1 (MQ=255)

                              tgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTAt                                                                     >  2:1532951‑M2/55‑99 (MQ=255)

                               gaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATc                                                                    >  1:1617068‑M2/54‑99 (MQ=255)

                               gaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATc                                                                    >  1:1957731‑M2/54‑99 (MQ=255)

                                ttGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATc                                                                    <  1:3184805/99‑1 (MQ=255)

                                aagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATcc                                                                   <  2:289514‑M2/47‑1 (MQ=255)

                                aagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATcc                                                                   >  2:2762337‑M2/53‑99 (MQ=255)

                                aagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATcc                                                                   >  2:445322‑M2/53‑99 (MQ=255)

                                  gaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGc                                                                 <  1:823999‑M2/49‑1 (MQ=255)

                                   aaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCt                                                                >  1:3654281‑M2/50‑99 (MQ=255)

                                    aaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTc                                                               >  2:3455223‑M2/49‑99 (MQ=255)

                                    aaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTc                                                               >  1:1413274‑M2/49‑99 (MQ=255)

                                         tgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAt                                                          >  2:2628724‑M2/44‑99 (MQ=255)

                                          gaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAtt                                                         <  2:131777‑M2/57‑1 (MQ=255)

                                           aatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTc                                                        >  2:3671860‑M2/42‑99 (MQ=255)

                                             tgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCca                                                      <  2:1602315‑M2/60‑1 (MQ=255)

                                               gTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcac                                                     >  1:2715369/1‑99 (MQ=255)

                                               atgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcaca                                                    <  1:561963‑M2/62‑1 (MQ=255)

                                               atgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcaca                                                    <  2:583210‑M2/62‑1 (MQ=255)

                                                tgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcacac                                                   >  1:3116968‑M2/37‑99 (MQ=255)

                                                tgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcacac                                                   >  1:2303482‑M2/37‑99 (MQ=255)

                                                tgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcacac                                                   >  1:1197623‑M2/37‑99 (MQ=255)

                                                  tagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACacaa                                                 >  2:2829866‑M2/35‑99 (MQ=255)

                                                  tagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACacaa                                                 <  1:216259‑M2/65‑1 (MQ=255)

                                                  tagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGAGTGAAATTGTTATCCGCTCACAATTCCACacaa                                                 >  2:3316062‑M2/35‑99 (MQ=255)

                                                      cgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATa                                             <  2:3813398‑M2/69‑1 (MQ=255)

                                                        tcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACg                                           >  2:1808869‑M2/29‑99 (MQ=255)

                                                          aagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAg                                         >  2:1389479‑M2/27‑99 (MQ=255)

                                                               gtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGa                                    >  2:3178668‑M2/22‑99 (MQ=255)

                                                                tcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGaa                                   >  2:3742872‑M2/21‑99 (MQ=255)

                                                                tcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGaa                                   >  1:1002747‑M2/21‑99 (MQ=255)

                                                                tcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGaa                                   >  1:1889085‑M2/21‑99 (MQ=255)

                                                                 cataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAg                                  >  2:2969434‑M2/20‑99 (MQ=255)

                                                                   taattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCa                                >  1:3385201‑M2/18‑99 (MQ=255)

                                                                   taattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCa                                <  2:2357346‑M2/82‑1 (MQ=255)

                                                                    aattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCAt                               <  2:1948522‑M2/83‑1 (MQ=255)

                                                                    aattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCAt                               >  2:1741027‑M2/17‑99 (MQ=255)

                                                                     attggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATa                              >  1:1546471‑M2/16‑99 (MQ=255)

                                                                     attggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATa                              <  2:724579‑M2/84‑1 (MQ=255)

                                                                         gtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAgt                          <  1:168533‑M2/88‑1 (MQ=255)

                                                                         gtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAgt                          >  2:516136‑M2/12‑99 (MQ=255)

                                                                         gtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAgt                          >  1:3588109‑M2/12‑99 (MQ=255)

                                                                          taccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAgtg                         >  1:1268379‑M2/11‑99 (MQ=255)

                                                                          taccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAgtg                         >  1:2912910‑M2/11‑99 (MQ=255)

                                                                            ccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTa                       <  2:2427344‑M2/91‑1 (MQ=255)

                                                                            ccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTa                       >  1:222576‑M2/9‑99 (MQ=255)

                                                                            ccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTa                       >  2:1025746‑M2/9‑99 (MQ=255)

                                                                             cgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTaa                      <  1:50140‑M2/92‑1 (MQ=255)

                                                                              gagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTaaa                     <  1:1131093‑M2/93‑1 (MQ=255)

                                                                              gagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTaaa                     >  2:2494629‑M2/7‑99 (MQ=255)

                                                                               agctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAg                    >  1:1479483‑M2/6‑99 (MQ=255)

                                                                                gctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGc                   >  1:2573772‑M2/5‑99 (MQ=255)

                                                                                 ctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGcc                  <  1:1818321‑M2/96‑1 (MQ=255)

                                                                                 ctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGCTATCCGCTCACAATTCCACACAACATACGAACCGGAAGCATAAAGTGTGAAGcc                  >  2:1243279‑M2/4‑99 (MQ=255)

                                                                                   cGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTg                <  1:3245903‑M2/98‑1 (MQ=255)

                                                                                     aaTTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTggg              >  2:2157032/1‑99 (MQ=255)

                                                                                      attcGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTgggg             >  1:957525/4‑99 (MQ=255)

                                                                                       ttcGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGt            >  1:629918/3‑99 (MQ=255)

                                                                                       ttcGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGt            >  2:794500/3‑99 (MQ=255)

                                                                                         cGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGc          <  1:2504608/99‑1 (MQ=255)

                                                                                          gTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGcc         <  2:3245968/99‑1 (MQ=255)

                                                                                          gTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGcc         <  2:3598673/99‑1 (MQ=255)

                                                                                          gTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGcc         >  1:1347005/1‑99 (MQ=255)

                                                                                          gTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGcc         <  1:3448179/99‑1 (MQ=255)

                                                                                          gTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGcc         >  2:2294887/1‑99 (MQ=255)

                                                                                           tAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCt        >  1:2841748/1‑99 (MQ=255)

                                                                                           tAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCt        >  1:3313759/1‑99 (MQ=255)

                                                                                            aaTCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTa       >  2:338548/1‑99 (MQ=255)

                                                                                             aTCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTaa      <  2:1670568/99‑1 (MQ=255)

GCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAG  >  NC_000913/366202‑366398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`