Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,535,612	A→C	I86S (ATC→AGC)	envZ ←	sensory histidine kinase EnvZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,535,612	0	A	C	100.0%	293.8 / NA	93	I86S (ATC→AGC)	envZ	sensory histidine kinase EnvZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (47/46);  total (47/46)

CGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATAC  >  NC_000913/3535517‑3535705                                                                                                |                                                                                              cgccCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTcc                                                                                            >  2:1405057/1‑99 (MQ=255) cgccCAGTTGCTGCGACATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAGCGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTcc                                                                                            <  2:1441496/99‑1 (MQ=255)   cccAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTcccc                                                                                          >  1:2036199/1‑99 (MQ=255)    ccAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTcccta                                                                                         <  2:1268025/99‑3 (MQ=255)    ccAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCa                                                                                         <  1:2366060/99‑1 (MQ=255)      aGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGc                                                                                       <  2:1810590/99‑1 (MQ=255)           ctgcGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACg                                                                                  <  1:1629344/99‑1 (MQ=255)             gcgcCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGt                                                                                <  1:114794/99‑1 (MQ=255)              cgcCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTa                                                                               <  2:961887/99‑1 (MQ=255)               gcCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAg                                                                              >  2:2276305/1‑99 (MQ=255)                  aTCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGAtc                                                                           <  2:1640022/99‑1 (MQ=255)                  aTCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGAtc                                                                           <  1:3282398/99‑1 (MQ=255)                   tCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGAtct                                                                          >  2:2365979/1‑99 (MQ=255)                       aTGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCg                                                                      >  2:3398021/1‑99 (MQ=255)                       aTGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCg                                                                      >  1:2434833/1‑99 (MQ=255)                        tGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGa                                                                     >  1:2691521/1‑99 (MQ=255)                        tGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGa                                                                     >  1:1262504/1‑99 (MQ=255)                         ggCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGAc                                                                    <  2:1567280/99‑1 (MQ=255)                         ggCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGAc                                                                    >  1:1898021/1‑99 (MQ=255)                          gCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACg                                                                   >  2:1497605/1‑99 (MQ=255)                          gCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACg                                                                   >  1:2904315/1‑99 (MQ=255)                          gCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACg                                                                   >  2:362526/1‑99 (MQ=255)                           cTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACgg                                                                  >  1:236806/1‑99 (MQ=255)                             tAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGaa                                                                >  2:10888/1‑99 (MQ=255)                               aGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAg                                                              >  1:2719753/1‑99 (MQ=255)                               aGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAg                                                              >  1:3548545/1‑99 (MQ=255)                               aGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAg                                                              >  2:2153395/1‑99 (MQ=255)                                gAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGc                                                             <  2:3663114/99‑1 (MQ=255)                                 aaTTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCg                                                            <  1:689564/99‑1 (MQ=255)                                  aTTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCgg                                                           >  1:2590248/1‑99 (MQ=255)                                  aTTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCgg                                                           >  1:180886/1‑99 (MQ=255)                                    tCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGa                                                         >  1:1811344/1‑99 (MQ=255)                                     cATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAg                                                        <  2:177400/99‑1 (MQ=255)                                       tAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGc                                                      >  2:3044539/1‑99 (MQ=255)                                        aGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGca                                                     <  2:2775960/99‑1 (MQ=255)                                        aGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGca                                                     <  2:705806/99‑1 (MQ=255)                                        aGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGca                                                     <  1:3273771/99‑1 (MQ=255)                                         gtgtTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGTCGGAAAGCGGGAGGcac                                                    <  1:872061/99‑1 (MQ=255)                                         gtgtTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGcac                                                    >  1:3119420/1‑99 (MQ=255)                                         gtgtTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGcac                                                    >  1:790683/1‑99 (MQ=255)                                          tgtTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGcaca                                                   >  1:1778223/1‑99 (MQ=255)                                           gtTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaa                                                  <  1:1865444/99‑1 (MQ=255)                                            ttGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaac                                                 <  1:502963/99‑1 (MQ=255)                                                gcCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaaccaac                                             <  2:3807910/99‑1 (MQ=255)                                                gcCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaaccaac                                             <  2:407932/99‑1 (MQ=255)                                                    aaCGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCg                                         >  2:1239903/1‑99 (MQ=255)                                                      cGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTg                                       >  2:2260538/1‑99 (MQ=255)                                                       gCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGc                                      <  1:2528712/99‑1 (MQ=255)                                                          gACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGt                                   >  1:3604340/1‑99 (MQ=255)                                                          gACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGt                                   >  1:1020897/1‑99 (MQ=255)                                                           aCCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtc                                  >  1:1315761/1‑99 (MQ=255)                                                           aCCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtc                                  <  1:2385768/99‑1 (MQ=255)                                                           aCCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtc                                  <  1:3568642/99‑1 (MQ=255)                                                           aCCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtc                                  <  1:1564251/99‑1 (MQ=255)                                                           aCCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtc                                  <  1:1570091/99‑1 (MQ=255)                                                           aCCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtc                                  >  2:3702647/1‑99 (MQ=255)                                                            ccTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcc                                 >  1:589695/1‑99 (MQ=255)                                                            ccTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcc                                 >  1:1942960/1‑99 (MQ=255)                                                             cTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcct                                >  1:2893160/1‑99 (MQ=255)                                                              tGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcctc                               <  1:2286353/99‑1 (MQ=255)                                                              tGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcctc                               >  1:3453921/1‑99 (MQ=255)                                                              tGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcctc                               <  2:1542320/99‑1 (MQ=255)                                                                ccTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCa                             <  2:231672/99‑1 (MQ=255)                                                                 ctctTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAg                            <  1:3342904/99‑1 (MQ=255)                                                                 ctctTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAg                            <  2:2563924/99‑1 (MQ=255)                                                                 ctctTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAg                            >  2:1587201/1‑99 (MQ=255)                                                                 ctctTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAg                            <  2:1289589/99‑1 (MQ=255)                                                                  tctTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGt                           <  1:3529695/99‑1 (MQ=255)                                                                  tctTCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGt                           >  1:1708456/1‑99 (MQ=255)                                                                    ttCGGCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTg                         >  2:2212966/1‑99 (MQ=255)                                                                       ggCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAg                      >  1:501457/1‑99 (MQ=255)                                                                        gCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGt                     >  2:1944261/1‑99 (MQ=255)                                                                        gCAGCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGt                     <  1:766873/99‑1 (MQ=255)                                                                           gCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTg                  >  2:3112111/1‑99 (MQ=255)                                                                           gCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTg                  >  1:717610/1‑99 (MQ=255)                                                                           gCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTg                  <  1:3112300/99‑1 (MQ=255)                                                                           gCCTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTg                  >  1:2833086/1‑99 (MQ=255)                                                                             cTCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTc                >  1:3476790/1‑99 (MQ=255)                                                                              tCGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCg               <  2:3489406/99‑1 (MQ=255)                                                                               cGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCgg              <  1:112621/99‑1 (MQ=255)                                                                               cGTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCgg              <  1:2202890/99‑1 (MQ=255)                                                                                gTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGt             >  2:2719193/1‑99 (MQ=255)                                                                                gTTGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGt             >  2:2042066/1‑99 (MQ=255)                                                                                gTTGAAGTAAAGATAGCTCCACAACTCACGGTAGATCTCCCGTCGGAAAGCGCGCGGCACAACCAACTGCGTCCCCTCCTCCAGTTGCACTTTGTCGGt             <  2:605104/99‑1 (MQ=255)                                                                                 ttGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtc            >  1:1913683/1‑99 (MQ=255)                                                                                  tGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtca           <  1:3298967/99‑1 (MQ=255)                                                                                  tGGAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtca           <  2:3592538/99‑1 (MQ=255)                                                                                   ggAGTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGtcat          <  2:862337/99‑1 (MQ=255)                                                                                      gTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCaa       <  2:2007458/99‑1 (MQ=255)                                                                                      gTAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCaa       <  1:2166369/99‑1 (MQ=255)                                                                                       tAGAGAGAGCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAAc      >  1:1650124/1‑99 (MQ=255)                                                                                        agagagagCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACa     <  1:986856/99‑1 (MQ=255)                                                                                        agagagagCTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACa     <  1:1486203/99‑1 (MQ=255)                                                                                         gagagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACAt    >  1:905955/1‑99 (MQ=255)                                                                                          agagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATa   <  1:3188115/99‑1 (MQ=255)                                                                                          agagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATa   >  1:13340/1‑99 (MQ=255)                                                                                           gagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATAc  >  1:775739/1‑99 (MQ=255)                                                                                           gagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATAc  <  2:3396352/99‑1 (MQ=255)                                                                                           gagagcTCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATAc  <  2:2908681/99‑1 (MQ=255)                                                                                                |                                                                                              CGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGCCTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATAC  >  NC_000913/3535517‑3535705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑