BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	366,290	C→T	94.8%	D6N (GAT→AAT)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,290	0	C	T	94.8%	13334.3 / inf	3724	D6N (GAT→AAT)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand): ref base C (101/94); new base T (1729/1800); total (1830/1894)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3729 total aligned reads displayed.

ACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGT  >  NC_000913/366192‑366388

  tgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCg                                                                                                  <  2:1814942‑M2/7‑1 (MQ=255)

  tgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCg                                                                                                  <  1:2799454‑M2/7‑1 (MQ=255)

  tgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCg                                                                                                  <  2:2049017‑M2/7‑1 (MQ=255)

   gcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGt                                                                                                 <  1:1123828‑M2/8‑1 (MQ=255)

    caccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTa                                                                                                <  1:2753756‑M2/9‑1 (MQ=255)

     accggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTaa                                                                                               <  2:1469533‑M2/10‑1 (MQ=255)

     accggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTaa                                                                                               >  1:504369‑M2/90‑99 (MQ=255)

      ccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAAt                                                                                              <  2:898827‑M2/11‑1 (MQ=255)

        ggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCa                                                                                            <  2:2266999‑M2/13‑1 (MQ=255)

           gccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAtgt                                                                                         <  2:701462‑M2/16‑2 (MQ=255)

             cagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATcatgtca                                                                                       <  1:978077‑M2/18‑4 (MQ=255)

             cagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATcatgtca                                                                                       <  2:1582126‑M2/18‑4 (MQ=255)

              agcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAAtcatgtcat                                                                                      >  1:1685055‑M2/81‑95 (MQ=255)

               gcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATa                                                                                    >  1:2302813‑M2/80‑99 (MQ=255)

                gggggATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATa                                                                                    >  1:404004/1‑99 (MQ=255)

                  gggATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGc                                                                                  <  2:1997848/99‑1 (MQ=255)

                  cgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtacctagctcGAATTCGTAATCAT‑GTCATAGCt                                                                                 <  2:498766‑M2/23‑1 (MQ=255)

                  cgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgaagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCt                                                                                 >  2:2379107‑M2/77‑99 (MQ=255)

                   gagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTg                                                                                <  1:1733638‑M2/24‑1 (MQ=255)

                   gagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTg                                                                                <  2:1120984‑M2/24‑1 (MQ=255)

                    agcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtactgagctcGAATTCGTAATCAT‑GTCATAGCTGt                                                                               >  2:2792928‑M2/75‑99 (MQ=255)

                    agcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGt                                                                               >  1:873064‑M2/75‑99 (MQ=255)

                      cgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGttt                                                                             <  2:582653‑M2/27‑1 (MQ=255)

                        tgctgcAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTc                                                                            >  1:1322293/1‑99 (MQ=255)

                        agttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTcc                                                                           >  2:2573277‑M2/71‑99 (MQ=255)

                        agttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTcc                                                                           >  2:2098575‑M2/71‑99 (MQ=255)

                            ccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCtgtg                                                                       <  2:1082206‑M2/33‑1 (MQ=255)

                              aaGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCtgtgt                                                                      >  1:817544/1‑99 (MQ=255)

                               tgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGa                                                                    >  2:2206501‑M2/64‑99 (MQ=255)

                               tgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGa                                                                    >  1:2651989‑M2/64‑99 (MQ=255)

                                gccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGaa                                                                   >  1:1884301‑M2/63‑99 (MQ=255)

                                   ggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAAtt                                                                >  1:1087901‑M2/60‑99 (MQ=255)

                                   gATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAAt                                                                 <  2:136951/99‑1 (MQ=255)

                                     ttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGt                                                              >  1:2437675‑M2/58‑99 (MQ=255)

                                     ttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGt                                                              <  1:153950‑M2/42‑1 (MQ=255)

                                     ttAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTg                                                               <  2:1650449/99‑1 (MQ=255)

                                       gtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTa                                                            <  2:2730026‑M2/44‑1 (MQ=255)

                                       gtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTa                                                            <  2:1708473‑M2/44‑1 (MQ=255)

                                          aagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATcc                                                         >  2:851843‑M2/53‑99 (MQ=255)

                                          aagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATcc                                                         <  1:1438239‑M2/47‑1 (MQ=255)

                                          aagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATcc                                                         <  1:934880‑M2/47‑1 (MQ=255)

                                          aagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATcc                                                         >  1:2248459‑M2/53‑99 (MQ=255)

                                           agaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCg                                                        >  2:39382‑M2/52‑99 (MQ=255)

                                            gaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGc                                                       >  1:1486213‑M2/51‑99 (MQ=255)

                                               aaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTca                                                    <  1:239411‑M2/52‑1 (MQ=255)

                                               aaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTca                                                    >  1:2208547‑M2/48‑99 (MQ=255)

                                                aagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTcac                                                   <  2:1869624‑M2/53‑1 (MQ=255)

                                                 agtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTcaca                                                  >  2:1419504‑M2/46‑99 (MQ=255)

                                                 agtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTcaca                                                  >  1:2053504‑M2/46‑99 (MQ=255)

                                                 agtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTcaca                                                  >  1:2164333‑M2/46‑99 (MQ=255)

                                                  gtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACaa                                                 <  1:2107507‑M2/55‑1 (MQ=255)

                                                  gtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACaa                                                 <  2:1955001‑M2/55‑1 (MQ=255)

                                                    gaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAtt                                               <  2:950688‑M2/57‑1 (MQ=255)

                                                    gaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAtt                                               <  1:1786427‑M2/57‑1 (MQ=255)

                                                      atgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTcc                                             >  1:296160‑M2/41‑99 (MQ=255)

                                                      aGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTc                                              >  1:768496/1‑99 (MQ=255)

                                                        gatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcac                                           <  1:619697‑M2/61‑1 (MQ=255)

                                                         atgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcaca                                          >  2:5460‑M2/38‑99 (MQ=255)

                                                          tgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcacac                                         <  2:1186547‑M2/63‑1 (MQ=255)

                                                           gtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcacaca                                        <  1:666435‑M2/64‑1 (MQ=255)

                                                            ttCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcacaca                                        <  1:459932/99‑1 (MQ=255)

                                                            tagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACacaa                                       >  2:1239153‑M2/35‑99 (MQ=255)

                                                              gccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACacaaca                                     <  1:1389784‑M2/67‑1 (MQ=255)

                                                               ccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACAt                                    <  1:1986097‑M2/68‑1 (MQ=255)

                                                                cgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATa                                   <  1:1273958‑M2/69‑1 (MQ=255)

                                                                cgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATa                                   <  1:1630261‑M2/69‑1 (MQ=255)

                                                                 gtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATAc                                  <  2:2145333‑M2/70‑1 (MQ=255)

                                                                  tcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACg                                 >  1:347770‑M2/29‑99 (MQ=255)

                                                                  tcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACg                                 >  1:2691213‑M2/29‑99 (MQ=255)

                                                                  tcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACg                                 >  1:1583620‑M2/29‑99 (MQ=255)

                                                                   caagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGa                                <  2:1535824‑M2/72‑1 (MQ=255)

                                                                    aagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAg                               <  2:2332556‑M2/73‑1 (MQ=255)

                                                                    aagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAg                               <  1:1182575‑M2/73‑1 (MQ=255)

                                                                    aagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAg                               >  2:1039099‑M2/27‑99 (MQ=255)

                                                                    aagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAg                               >  2:1762778‑M2/27‑99 (MQ=255)

                                                                     agttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGc                              >  1:1934916‑M2/26‑99 (MQ=255)

                                                                     agttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGc                              <  2:44280‑M2/74‑1 (MQ=255)

                                                                      gttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGcc                             >  2:2624377‑M2/25‑99 (MQ=255)

                                                                      gttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTCATCCGCTCACAATTCCACACAACATACGAGcc                             >  1:2559098‑M2/25‑99 (MQ=255)

                                                                         gtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGa                          >  1:724042‑M2/22‑99 (MQ=255)

                                                                          tcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGaa                         >  2:2840145‑M2/21‑99 (MQ=255)

                                                                            ataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGc                       >  1:575400‑M2/19‑99 (MQ=255)

                                                                             taattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCa                      >  2:1317279‑M2/18‑99 (MQ=255)

                                                                             taattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCa                      <  2:2677813‑M2/82‑1 (MQ=255)

                                                                             taattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCa                      <  2:2524258‑M2/82‑1 (MQ=255)

                                                                               attggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATa                    <  2:2428829‑M2/84‑1 (MQ=255)

                                                                                ttggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATaa                   <  1:1834559‑M2/85‑1 (MQ=255)

                                                                                ttggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATaa                   <  2:746729‑M2/85‑1 (MQ=255)

                                                                                aaaCGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATa                    <  2:1918906/99‑1 (MQ=255)

                                                                                 tggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATaaa                  <  1:2349731‑M2/86‑1 (MQ=255)

                                                                                      ccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTa             <  2:635673‑M2/91‑1 (MQ=255)

                                                                                      ccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTa             >  1:1242823‑M2/9‑99 (MQ=255)

                                                                                       cgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTaa            <  1:2511302‑M2/92‑1 (MQ=255)

                                                                                       cgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTaa            <  2:803527‑M2/92‑1 (MQ=255)

                                                                                         agctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAg          <  1:1042905‑M2/94‑1 (MQ=255)

                                                                                          gctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGc         <  1:247559‑M2/95‑1 (MQ=255)

                                                                                           ctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGcc        >  2:2466376‑M2/4‑99 (MQ=255)

                                                                                            tcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCt       <  2:340377‑M2/97‑1 (MQ=255)

                                                                                              gAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTgg     >  1:1001334/1‑99 (MQ=255)

                                                                                               aaTTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTggg    >  1:2612863/1‑99 (MQ=255)

ACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGT  >  NC_000913/366192‑366388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`