| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,535,733 | A→C | 100% | F46V (TTT→GTT) | envZ ← | sensory histidine kinase EnvZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,535,733 | 0 | A | C | 100.0% | 271.3 / NA | 81 | F46V (TTT→GTT) | envZ | sensory histidine kinase EnvZ |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (42/39); total (42/39) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAA > NC_000913/3535637‑3535823 | gACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACct > 1:2532175/1‑99 (MQ=255)gACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACct > 2:1891295/1‑99 (MQ=255) aCGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACctg > 2:769013/1‑99 (MQ=255) aCGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACctg > 1:263165/1‑99 (MQ=255) aCGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACctg < 2:2036647/99‑1 (MQ=255) ggAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACctgct > 2:1369187/1‑99 (MQ=255) ggAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTACTAACctgct < 1:149358/99‑1 (MQ=255) aGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAg < 2:1790811/99‑1 (MQ=255) gCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAgg > 1:2043174/1‑99 (MQ=255) cGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGc < 1:1565910/99‑1 (MQ=255) gggAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCt < 1:2031097/99‑1 (MQ=255) ggAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTc > 1:1850435/1‑99 (MQ=255) gAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCg > 1:1067855/1‑99 (MQ=255) gCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCa > 2:590714/1‑99 (MQ=255) caaccaacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCaaaa < 2:2054160/99‑1 (MQ=255) caaccaacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCaaaa > 2:13629/1‑99 (MQ=255) caaccaacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCaaaa > 2:1163275/1‑99 (MQ=255) caaccaacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCaaaa > 1:1250658/1‑99 (MQ=255) caaccaacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCaaaa < 1:1990426/99‑1 (MQ=255) accaacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATc > 2:2055013/1‑99 (MQ=255) accaacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATc > 2:955219/1‑99 (MQ=255) aacTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATcgcg < 2:967752/99‑1 (MQ=255) cTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGaa > 2:1119087/1‑99 (MQ=255) cTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGaa < 1:799107/99‑1 (MQ=255) cGTCCTCCAGTTGCAGTTTGTCGTTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGca < 2:855491/99‑1 (MQ=255) cGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGca < 1:816110/99‑1 (MQ=255) cGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGca > 1:772215/1‑99 (MQ=255) gTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGcac < 2:436323/99‑1 (MQ=255) tcctccAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGcacc > 2:1465436/1‑99 (MQ=255) ctccAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGcaccac < 1:1025438/99‑1 (MQ=255) tccAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCaccacc > 2:2436711/1‑99 (MQ=255) ccAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCAccacca < 2:1161624/99‑1 (MQ=255) ttGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATa < 1:1803764/99‑1 (MQ=255) ttGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATa > 2:1698344/1‑99 (MQ=255) gCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAg < 1:1182757/99‑1 (MQ=255) cAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAgt < 2:18957/99‑1 (MQ=255) tttGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAgtcgt > 1:144532/1‑99 (MQ=255) tttGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAgtcgt > 2:1912227/1‑99 (MQ=255) ttGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAgtcgtc < 1:925581/99‑1 (MQ=255) gTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCAc > 1:652057/1‑99 (MQ=255) ggTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAg < 1:1385386/99‑1 (MQ=255) gTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAgg < 2:2446100/99‑1 (MQ=255) gTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAgg > 1:2226381/1‑99 (MQ=255) tcatcaACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGTAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGc < 2:860777/99‑1 (MQ=255) tcatcaACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGc < 2:1113811/99‑1 (MQ=255) tcatcaACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGc < 2:1506849/99‑1 (MQ=255) tcatcaACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGc < 2:1295509/99‑1 (MQ=255) tcaACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTgg < 2:676887/99‑1 (MQ=255) tcaACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTgg > 1:1895557/1‑99 (MQ=255) tcaACATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTgg < 2:2573714/99‑1 (MQ=255) aCATACGCACTTCGTACGCGAGGACTTTTTTAACCTCCTGGAGCCTCGCCAAAATCGCGAAGTTCGGCACCACCAGAAAAGTCGCCACCAGGCTGGCGa > 1:2025291/1‑99 (MQ=255) aCATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGa > 1:1614675/1‑99 (MQ=255) cATACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGaa < 2:1448130/99‑1 (MQ=255) aTACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAAc < 1:1734504/99‑1 (MQ=255) taggCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACa > 1:1606723/4‑99 (MQ=255) tACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACa > 1:1606722/1‑99 (MQ=255) tACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACa > 1:742768/1‑99 (MQ=255) tACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACa < 1:827181/99‑1 (MQ=255) tACGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACa > 2:36722/1‑99 (MQ=255) cGCACTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGc < 1:962421/99‑1 (MQ=255) aCTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAg < 1:629216/99‑1 (MQ=255) cTTCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAgg > 1:2480170/1‑99 (MQ=255) ttCGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGt > 1:1294911/1‑99 (MQ=255) cGTACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGa < 2:2498635/99‑1 (MQ=255) tACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACg > 1:1444801/1‑99 (MQ=255) tACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACg > 1:1306751/1‑99 (MQ=255) tACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACg > 1:1706212/1‑99 (MQ=255) tACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACg > 2:1849401/1‑99 (MQ=255) tACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACg > 1:1311373/1‑99 (MQ=255) tACGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACg > 2:2541750/1‑99 (MQ=255) aCGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGa < 2:1391450/99‑1 (MQ=255) aCGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGa < 1:1347398/99‑1 (MQ=255) aCGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGa < 1:948133/99‑1 (MQ=255) aCGCGAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGa < 2:1750289/99‑1 (MQ=255) gAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAg > 2:1509947/1‑99 (MQ=255) gAGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAg > 1:2355773/1‑99 (MQ=255) aGGACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGc > 1:942210/1‑99 (MQ=255) ggACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCa < 1:2228945/99‑1 (MQ=255) gACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCaa > 2:2017093/1‑99 (MQ=255) gACTTTATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCaa < 2:1552501/99‑1 (MQ=255) tttATTAACCTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATaa < 1:190438/99‑1 (MQ=255) | GACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCCTCCAGTTGCAGTTTGTCGGTCATCAACATACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAA > NC_000913/3535637‑3535823 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |