BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	67,872	C→T	27.8%	A156T (GCC→ACC)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,872	0	C	T	27.8%	133.1 / 60.9	104	A156T (GCC→ACC)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base C (46/29); new base T (18/11); total (64/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 106 total aligned reads displayed.

CACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTT  >  NC_000913/67774‑67968

cACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGc                                                                                                  <  2:678928/99‑1 (MQ=255)

  cTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGttt                                                                                                >  1:2727364/1‑99 (MQ=255)

  cTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCtt                                                                                                >  1:1803445/1‑99 (MQ=255)

   ttCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGTTTg                                                                                               <  2:1974211/99‑1 (MQ=255)

   ttCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTg                                                                                               <  1:3414096/99‑1 (MQ=255)

    tCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGt                                                                                              <  1:2058416/99‑1 (MQ=255)

       cGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTa                                                                                           >  2:1881461/1‑99 (MQ=255)

        gCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTAt                                                                                          >  1:2373809/1‑99 (MQ=255)

         cATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATc                                                                                         <  1:1161614/99‑1 (MQ=255)

         cATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATc                                                                                         <  2:3170428/99‑1 (MQ=255)

          aTGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATcc                                                                                        <  2:2004815/99‑1 (MQ=255)

          aTGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATcc                                                                                        >  1:2739841/1‑99 (MQ=255)

            gTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTg                                                                                      >  1:158574/1‑99 (MQ=255)

             ttATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGc                                                                                     >  2:1810170/1‑99 (MQ=255)

                tCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAg                                                                                  <  2:1156855/99‑1 (MQ=255)

                tCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAg                                                                                  >  2:178413/1‑99 (MQ=255)

                tCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAg                                                                                  >  2:1127149/1‑99 (MQ=255)

                 cGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGt                                                                                 <  1:1928957/99‑1 (MQ=255)

                      aaTCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCg                                                                            >  1:561559/1‑99 (MQ=255)

                       aTCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCgg                                                                           >  1:2175514/1‑99 (MQ=255)

                         cGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTa                                                                         <  1:322074/99‑1 (MQ=255)

                          ggCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTaa                                                                        <  2:2502826/99‑1 (MQ=255)

                           gCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAAc                                                                       >  1:3044911/1‑99 (MQ=255)

                           gCACACTTTCAGATGACGGGTACCTTGGGTGGAGACCGCCTGGGGCATCCAGGAGCCGTTCCGCTCAGGGGCTTGTTTATACTGCCCGTAACCGGTAAc                                                                       >  2:2979194/1‑99 (MQ=255)

                            cAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAAcc                                                                      >  2:1055681/1‑99 (MQ=255)

                             aGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCa                                                                     <  1:2824095/99‑1 (MQ=255)

                             aGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCa                                                                     >  2:3210003/1‑99 (MQ=255)

                              gACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCAc                                                                    >  2:1328643/1‑99 (MQ=255)

                              gACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCAc                                                                    >  2:3235214/1‑99 (MQ=255)

                              gACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCAc                                                                    <  2:2450771/99‑1 (MQ=255)

                              gACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCAc                                                                    >  1:2561893/1‑99 (MQ=255)

                                  ttCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCa                                                                <  2:2954850/99‑1 (MQ=255)

                                    cAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATg                                                              >  2:1443594/1‑99 (MQ=255)

                                     aGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGAAACGCTCATGGGTTTGTTTATCCTGCCAGGTACCGGTAACCACGGCATGt                                                             >  2:2529037/1‑99 (MQ=255)

                                      gATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGtt                                                            >  1:2328773/1‑99 (MQ=255)

                                       aTGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTg                                                           <  1:2522284/99‑1 (MQ=255)

                                       aTGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTg                                                           >  1:3069318/1‑99 (MQ=255)

                                       aTGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTg                                                           >  2:2673724/1‑99 (MQ=255)

                                        tGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGc                                                          >  1:1041306/1‑99 (MQ=255)

                                        tGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGc                                                          >  2:1973708/1‑99 (MQ=255)

                                         gACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCt                                                         >  1:804069/1‑99 (MQ=255)

                                          aCGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTg                                                        >  1:2009950/1‑99 (MQ=255)

                                           tgggTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGa                                                       >  1:1425941/2‑99 (MQ=255)

                                           cGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGa                                                       <  1:2410558/99‑1 (MQ=255)

                                              gTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGc                                                    >  1:1410214/1‑99 (MQ=255)

                                               tATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCa                                                   >  1:2970244/1‑99 (MQ=255)

                                               tATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCa                                                   >  2:1630100/1‑99 (MQ=255)

                                               tATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCa                                                   >  1:2703132/1‑99 (MQ=255)

                                                 tCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATa                                                 <  2:1955292/99‑1 (MQ=255)

                                                 tCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATa                                                 <  2:2780178/99‑1 (MQ=255)

                                                 tCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATa                                                 <  1:1237733/99‑1 (MQ=255)

                                                    tGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATAcgc                                              >  2:1218572/1‑99 (MQ=255)

                                                    tGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATAcgc                                              >  1:1043851/1‑99 (MQ=255)

                                                     gTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATAcgcg                                             <  2:1967729/99‑1 (MQ=255)

                                                      tttAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATAcgcgc                                            >  1:1946506/1‑99 (MQ=255)

                                                      tttAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATAcgcgc                                            >  1:2196243/1‑99 (MQ=255)

                                                      tttAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATAcgcgc                                            >  2:1551588/1‑99 (MQ=255)

                                                      tctAGAGACCTCCTGACGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTTCCGGTGGCCGGTTACCCCTGCATGTTGCTGGCGCTTAcgccc                                            >  2:1274849/3‑97 (MQ=255)

                                                        tAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACgcgcgc                                          >  1:2549498/1‑99 (MQ=255)

                                                              ccGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGa                                    >  1:601257/1‑99 (MQ=255)

                                                              ccGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGa                                    <  1:1639244/99‑1 (MQ=255)

                                                              ccGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGa                                    >  1:3004456/1‑99 (MQ=255)

                                                               cGCCTGACGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGaa                                   >  2:915451/1‑99 (MQ=255)

                                                               cGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGaa                                   <  2:187312/99‑1 (MQ=255)

                                                                gCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAg                                  >  1:3252642/1‑99 (MQ=255)

                                                                gCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAg                                  <  1:1522877/99‑1 (MQ=255)

                                                                 ccTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGc                                 >  2:3143180/1‑99 (MQ=255)

                                                                    gACGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGa                              <  2:1575169/99‑1 (MQ=255)

                                                                    gACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCCGACGCATACGCGCGCCAATGAAGCCGa                              >  1:1554685/1‑99 (MQ=255)

                                                                     aggCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGaa                             <  1:962521/97‑1 (MQ=255)

                                                                     aCGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGaa                             <  2:1819347/99‑1 (MQ=255)

                                                                      cGCATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAAc                            <  1:2015538/99‑1 (MQ=255)

                                                                        cATCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTc                          >  1:2597061/1‑99 (MQ=255)

                                                                        cATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTc                          >  1:570494/1‑99 (MQ=255)

                                                                        cATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTc                          <  1:243331/99‑1 (MQ=255)

                                                                         aTCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTcg                         >  1:1890731/1‑99 (MQ=255)

                                                                          tCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTcgc                        >  2:2195091/1‑99 (MQ=255)

                                                                          tCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTcgc                        <  2:1657388/99‑1 (MQ=255)

                                                                          tCCAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTcgc                        >  2:1064428/1‑99 (MQ=255)

                                                                          tCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTcgc                        >  1:434985/1‑99 (MQ=255)

                                                                          tCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTcgc                        >  1:193252/1‑99 (MQ=255)

                                                                            cAGGAGCCGATACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGa                      >  2:761119/1‑99 (MQ=255)

                                                                              ggAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGAcc                    >  1:2927458/1‑99 (MQ=255)

                                                                                    gATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCAtg              >  1:3356173/1‑99 (MQ=255)

                                                                                    gATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCAtg              <  2:2088679/99‑1 (MQ=255)

                                                                                     aTACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCAtgt             >  1:2032318/1‑99 (MQ=255)

                                                                                     aTACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCAtgt             >  1:2383701/1‑99 (MQ=255)

                                                                                      tACGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCAtgtg            <  2:1078306/99‑1 (MQ=255)

                                                                                      tACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCAtgtg            >  2:3080500/1‑99 (MQ=255)

                                                                                        cGCTCATGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCa          <  1:2509829/99‑1 (MQ=255)

                                                                                        cGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCa          <  1:2151629/99‑1 (MQ=255)

                                                                                        cGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCa          <  2:1808574/99‑1 (MQ=255)

                                                                                        cGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCa          >  2:1795219/1‑99 (MQ=255)

                                                                                          cTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGt        <  1:3240171/99‑1 (MQ=255)

                                                                                           tCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTc       >  2:2003610/1‑99 (MQ=255)

                                                                                            cATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCt      <  2:149696/99‑1 (MQ=255)

                                                                                             aTGGGTTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTg     <  2:124238/99‑1 (MQ=255)

                                                                                             aTGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTg     >  1:179103/1‑99 (MQ=255)

                                                                                              tGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTgg    >  1:3002990/1‑99 (MQ=255)

                                                                                                ggCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTggtt  <  1:1457376/99‑1 (MQ=255)

CACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTT  >  NC_000913/67774‑67968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`