Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	3,548,550	24 bp→TTTTTTTTTAATGATACGGCGACC	6.5%	coding (1498‑1521/2085 nt)	malQ ←	4‑alpha‑glucanotransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3548549	58 (0.820)	3 (0.060) +TTTTTTTTTAATGATACGGCGACC	3/128	NT	6.5%	coding (1522/2085 nt)	malQ	4‑alpha‑glucanotransferase
	?	NC_000913	3548574 =	62 (0.870)					coding (1497/2085 nt)	malQ	4‑alpha‑glucanotransferase

GCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3548513‑3548549 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGAT  >  NC_000913/3548574‑3548641                                      ||||||||||||||||||||||||                                                                     GCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACCAATCACCATACAGCGATGACGTT                                <  2:230520/99‑1    CTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACCAATCACCATACAGCGATGACGTTTAC                             <  2:1208720/99‑1                               GCAGCTTATCTTCTTTCATGATCCGTCCACCACCGAGATCTACACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGAT  <  2:1485308/99‑1                                      ||||||||||||||||||||||||                                                                     GCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3548513‑3548549 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGAT  >  NC_000913/3548574‑3548641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.