Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	991,141	G→C	100%	V174L (GTA→CTA)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	991,141	0	G	C	100.0%	271.6 / NA	71	V174L (GTA→CTA)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (40/31);  total (40/31)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTC  >  NC_000913/991045‑991235                                                                                                 |                                                                                               accaccAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTa                                                                                              <  1:1253399/99‑1 (MQ=255)    accaAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAg                                                                                           <  2:223038/99‑1 (MQ=255)     ccaAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGt                                                                                          >  1:1286502/1‑99 (MQ=255)      caAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTg                                                                                         >  1:759736/1‑99 (MQ=255)        aaaTTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGc                                                                                       >  2:484268/1‑99 (MQ=255)           ttattGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAgg                                                                                    >  1:137420/1‑99 (MQ=255)             attGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGAc                                                                                  >  1:1522325/1‑99 (MQ=255)                   gATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTc                                                                            >  1:1217259/1‑99 (MQ=255)                    aTAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTcc                                                                           <  2:1533975/99‑1 (MQ=255)                    aTAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTcc                                                                           <  1:1554992/99‑1 (MQ=255)                       aaaTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGa                                                                        >  2:1260287/1‑99 (MQ=255)                       aaaTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGa                                                                        >  1:1253636/1‑99 (MQ=255)                        aaTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGaa                                                                       >  2:172083/1‑99 (MQ=255)                        aaTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGaa                                                                       >  1:513330/1‑99 (MQ=255)                        aaTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGaa                                                                       >  1:260470/1‑99 (MQ=255)                         aTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGaaa                                                                      <  1:89031/99‑1 (MQ=255)                          tCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAAc                                                                     >  1:279417/1‑99 (MQ=255)                           cAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAAcc                                                                    <  1:1804035/99‑1 (MQ=255)                             aaTATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGt                                                                  >  1:1153173/1‑99 (MQ=255)                             aaTATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGt                                                                  <  1:1744505/99‑1 (MQ=255)                             aaTATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGt                                                                  >  1:1194875/1‑99 (MQ=255)                                atCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCt                                                               >  2:39015/1‑99 (MQ=255)                                atCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCt                                                               <  1:1291874/99‑1 (MQ=255)                                 tCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTa                                                              >  2:1382099/1‑99 (MQ=255)                                  cccTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTAc                                                             >  1:1915922/1‑99 (MQ=255)                                   ccTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTAcc                                                            >  2:1519395/1‑99 (MQ=255)                                    cTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCt                                                           >  2:326929/1‑99 (MQ=255)                                    cTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCt                                                           <  2:1921215/99‑1 (MQ=255)                                     ttCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTg                                                          <  2:1958772/99‑1 (MQ=255)                                     ttCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTg                                                          >  1:177915/1‑99 (MQ=255)                                         tGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTg                                                      <  1:796955/99‑1 (MQ=255)                                           cTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTgcg                                                    >  2:1609782/1‑99 (MQ=255)                                            ttgccAATGGTAACCGCGTTGCGCAAGTCGAAATAGAAAACGGACGCTATTGGCTACAGTGGCAGGATCCGTTCCCGAAGCCGTGCTACCTGTTTgcgc                                                   <  2:1567132/96‑1 (MQ=255)                                               ccAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCtgg                                                <  2:1292603/99‑1 (MQ=255)                                               ccAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCtgg                                                <  1:704478/99‑1 (MQ=255)                                               ccAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCtgg                                                <  2:748346/99‑1 (MQ=255)                                                 aaTGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCtggtg                                              <  1:1017311/99‑1 (MQ=255)                                                  aTGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCtggtgg                                             >  2:682300/1‑99 (MQ=255)                                                   tGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGc                                            <  2:614178/99‑1 (MQ=255)                                                   tGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGc                                            >  2:675553/1‑99 (MQ=255)                                                   tGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGc                                            >  2:938020/1‑99 (MQ=255)                                                   tGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGc                                            >  1:1227632/1‑99 (MQ=255)                                                     gTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAg                                          >  1:1259751/1‑99 (MQ=255)                                                      tAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAgg                                         >  1:1857663/1‑99 (MQ=255)                                                       aaCCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGc                                        <  1:599846/99‑1 (MQ=255)                                                        aCCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCg                                       >  1:427681/1‑99 (MQ=255)                                                        aCCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCg                                       >  2:652994/1‑99 (MQ=255)                                                         ccGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGa                                      <  2:208443/99‑1 (MQ=255)                                                          cgcgTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGAc                                     <  2:1856913/99‑1 (MQ=255)                                                            ggTTGCGCAAGGCGAACTGGAAAACGTACACCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCAGTTTGCGCTGGTTGCAGGCGACtt                                   <  2:1801400/98‑1 (MQ=255)                                                            cgTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACtt                                   <  2:327660/99‑1 (MQ=255)                                                             gTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACttt                                  <  2:749046/99‑1 (MQ=255)                                                              ttGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTg                                 >  1:882844/1‑99 (MQ=255)                                                               tGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGa                                <  2:1487899/99‑1 (MQ=255)                                                               tGCGCAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGa                                <  1:278232/99‑1 (MQ=255)                                                                gcgcAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGAt                               <  2:1817777/99‑1 (MQ=255)                                                                 cgcAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATg                              <  1:166734/99‑1 (MQ=255)                                                                  gcAAGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGt                             >  1:294131/1‑99 (MQ=255)                                                                     aGGCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACt                          <  1:371784/99‑1 (MQ=255)                                                                      ggCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTg                         >  2:1578869/1‑99 (MQ=255)                                                                       gCGAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTgc                        >  2:1774437/1‑99 (MQ=255)                                                                         gAACTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTgcgc                      <  1:927381/99‑1 (MQ=255)                                                                          aaCTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGcgcg                     >  1:1930876/1‑99 (MQ=255)                                                                          aaCTGGAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGcgcg                     >  2:993571/1‑99 (MQ=255)                                                                              ggAAAACGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATAc                 <  1:1447908/99‑1 (MQ=255)                                                                                aaaaCGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCt               >  2:302965/1‑99 (MQ=255)                                                                                 aaaCGGACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCtt              >  1:1474474/1‑99 (MQ=255)                                                                                      gACGCCATTGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCa         >  1:770011/1‑99 (MQ=255)                                                                                             ttGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTc  <  2:1192976/99‑1 (MQ=255)                                                                                             ttGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTc  >  2:701862/1‑99 (MQ=255)                                                                                             ttGGCTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTc  >  1:1622156/1‑99 (MQ=255)                                                                                                 |                                                                                               ACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTC  >  NC_000913/991045‑991235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑