BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	366,301	Δ1 bp	coding (5/3075 nt)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,300	0	G	.	97.3%	21137.9 / inf	4905	coding (6/3075 nt)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand): ref base G (53/78); new base . (2419/2352); total (2475/2430)
Fisher's exact test for biased strand distribution p-value = 2.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5175 total aligned reads displayed.

GCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAG  >  NC_000913/366202‑366398

 gccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAtgt                                                                                                   >  1:2622180‑M2/84‑98 (MQ=255)

   cagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATcatgtca                                                                                                 <  2:841143‑M2/18‑4 (MQ=255)

   cagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATcatgtca                                                                                                 <  2:1065801‑M2/18‑4 (MQ=255)

    agcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAAtcatgtcat                                                                                                >  1:2398400‑M2/81‑95 (MQ=255)

    agcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAAtcatgtcat                                                                                                >  1:969443‑M2/81‑95 (MQ=255)

    aaGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCa                                                                                                <  2:2499369/99‑1 (MQ=255)

     gcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATa                                                                                              >  2:571329‑M2/80‑99 (MQ=255)

     gcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATa                                                                                              >  1:2571800‑M2/80‑99 (MQ=255)

     gcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATa                                                                                              <  2:2607428‑M2/20‑1 (MQ=255)

       ccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGc                                                                                            <  2:1734015‑M2/22‑1 (MQ=255)

        cgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCt                                                                                           >  2:1951588‑M2/77‑99 (MQ=255)

         gagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTg                                                                                          >  2:150865‑M2/76‑99 (MQ=255)

         gagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTg                                                                                          >  1:389834‑M2/76‑99 (MQ=255)

             gagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTc                                                                                      <  1:547101‑M2/28‑1 (MQ=255)

             gagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTc                                                                                      <  1:1553031‑M2/28‑1 (MQ=255)

              agttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTcc                                                                                     <  2:627639‑M2/29‑1 (MQ=255)

               gttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCt                                                                                    <  1:2533293‑M2/30‑1 (MQ=255)

                ttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCtg                                                                                   >  1:1972907‑M2/69‑99 (MQ=255)

                ttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCtg                                                                                   >  1:2130690‑M2/69‑99 (MQ=255)

                  ccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCtgtg                                                                                 <  1:605362‑M2/33‑1 (MQ=255)

                   cgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCtgtgt                                                                                <  1:2017640‑M2/34‑1 (MQ=255)

                    aaGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCtgtgt                                                                                <  2:323666/99‑1 (MQ=255)

                     tgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGa                                                                              <  2:1212694‑M2/36‑1 (MQ=255)

                       ccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGaaa                                                                            >  1:2675479‑M2/62‑99 (MQ=255)

                        cggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAAt                                                                           <  2:2088403‑M2/39‑1 (MQ=255)

                        cggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAAt                                                                           <  2:1552108‑M2/39‑1 (MQ=255)

                         ggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAAtt                                                                          <  1:442854‑M2/40‑1 (MQ=255)

                         ggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAAtt                                                                          <  1:124982‑M2/40‑1 (MQ=255)

                          gttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTg                                                                         >  2:2294714‑M2/59‑99 (MQ=255)

                          gttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTg                                                                         >  1:1044201‑M2/59‑99 (MQ=255)

                             gtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTa                                                                      <  2:1106445‑M2/44‑1 (MQ=255)

                             gtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTa                                                                      <  2:1416723‑M2/44‑1 (MQ=255)

                             gtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAAACAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTa                                                                      <  1:2221839‑M2/44‑1 (MQ=255)

                              tgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTAt                                                                     >  1:1453668‑M2/55‑99 (MQ=255)

                              tgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTAt                                                                     >  2:1316797‑M2/55‑99 (MQ=255)

                               gaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATc                                                                    <  1:1346322‑M2/46‑1 (MQ=255)

                                 agaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCg                                                                  >  1:507422‑M2/52‑99 (MQ=255)

                                 agaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCg                                                                  >  1:169787‑M2/52‑99 (MQ=255)

                                  gaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGc                                                                 <  2:1643078‑M2/49‑1 (MQ=255)

                                  gaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGc                                                                 >  1:2486540‑M2/51‑99 (MQ=255)

                                  gaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGc                                                                 >  2:1777832‑M2/51‑99 (MQ=255)

                                  gaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGc                                                                 >  2:2653355‑M2/51‑99 (MQ=255)

                                   aaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCt                                                                >  2:2458603‑M2/50‑99 (MQ=255)

                                   aaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCt                                                                <  2:1817915‑M2/50‑1 (MQ=255)

                                    aaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTc                                                               >  1:2177741‑M2/49‑99 (MQ=255)

                                    aaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTc                                                               >  2:1860346‑M2/49‑99 (MQ=255)

                                     tAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTc                                                               >  2:2147721/1‑99 (MQ=255)

                                     aaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTca                                                              <  1:1792778‑M2/52‑1 (MQ=255)

                                     aaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTca                                                              >  1:244574‑M2/48‑99 (MQ=255)

                                      aagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTcac                                                             >  1:1740359‑M2/47‑99 (MQ=255)

                                         tgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAt                                                          >  2:1904146‑M2/44‑99 (MQ=255)

                                         tgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAt                                                          <  1:1151285‑M2/56‑1 (MQ=255)

                                            atgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTcc                                                       >  2:470175‑M2/41‑99 (MQ=255)

                                             tgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCca                                                      <  2:1369787‑M2/60‑1 (MQ=255)

                                             tgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCca                                                      >  2:168481‑M2/40‑99 (MQ=255)

                                               atgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcaca                                                    <  1:35369‑M2/62‑1 (MQ=255)

                                                tgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcacac                                                   <  1:314407‑M2/63‑1 (MQ=255)

                                                tgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcacac                                                   >  1:1208253‑M2/37‑99 (MQ=255)

                                                 gtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcacaca                                                  <  1:2262200‑M2/64‑1 (MQ=255)

                                                     ccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACAt                                              <  1:1096642‑M2/68‑1 (MQ=255)

                                                     ccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACAt                                              <  2:711394‑M2/68‑1 (MQ=255)

                                                      cgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATa                                             >  1:1500940‑M2/31‑99 (MQ=255)

                                                      cgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATa                                             <  2:2192392‑M2/69‑1 (MQ=255)

                                                      cgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATa                                             >  1:1292081‑M2/31‑99 (MQ=255)

                                                        tcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACg                                           >  2:1154525‑M2/29‑99 (MQ=255)

                                                        tcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACg                                           >  2:802277‑M2/29‑99 (MQ=255)

                                                         caagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGa                                          <  1:2355893‑M2/72‑1 (MQ=255)

                                                         caagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGa                                          <  1:1606912‑M2/72‑1 (MQ=255)

                                                          aagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAg                                         <  2:278255‑M2/73‑1 (MQ=255)

                                                          aagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAg                                         <  2:2039780‑M2/73‑1 (MQ=255)

                                                           agttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGc                                        <  2:426244‑M2/74‑1 (MQ=255)

                                                             ttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCg                                      <  2:754805‑M2/76‑1 (MQ=255)

                                                                 cataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAg                                  <  2:1993333‑M2/80‑1 (MQ=255)

                                                                  ataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGc                                 <  1:1653446‑M2/81‑1 (MQ=255)

                                                                   taattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCa                                >  1:752325‑M2/18‑99 (MQ=255)

                                                                        ggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAg                           <  2:888844‑M2/87‑1 (MQ=255)

                                                                        ggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAg                           <  2:947659‑M2/87‑1 (MQ=255)

                                                                         gtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAgt                          >  1:1882215‑M2/12‑99 (MQ=255)

                                                                            ccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTa                       <  2:659880‑M2/91‑1 (MQ=255)

                                                                             cgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTaa                      <  1:100010‑M2/92‑1 (MQ=255)

                                                                              gagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTaaa                     <  2:146540‑M2/93‑1 (MQ=255)

                                                                               agctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAg                    >  2:5071‑M2/6‑99 (MQ=255)

                                                                               agctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAg                    >  2:372041‑M2/6‑99 (MQ=255)

                                                                                gctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGTAGCATAAAGTGTAAAGc                   >  1:86978‑M2/5‑99 (MQ=255)

                                                                                gctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGc                   <  2:2346189‑M2/95‑1 (MQ=255)

                                                                                gctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGc                   <  1:918808‑M2/95‑1 (MQ=255)

                                                                                 ctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGcc                  <  1:815675‑M2/96‑1 (MQ=255)

                                                                                 ctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGcc                  <  1:2316526‑M2/96‑1 (MQ=255)

                                                                                 ctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGcc                  <  1:1399098‑M2/96‑1 (MQ=255)

                                                                                   cGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTg                <  2:2561145‑M2/98‑1 (MQ=255)

                                                                                    gAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTgg               >  1:1925040/1‑99 (MQ=255)

                                                                                     aaTTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTggg              >  1:1831162/1‑99 (MQ=255)

                                                                                      attcGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTgggg             <  2:1601144/96‑1 (MQ=255)

                                                                                      attcGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTgggg             <  1:2073294/96‑1 (MQ=255)

                                                                                      attcGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTgggg             <  2:239904/96‑1 (MQ=255)

                                                                                       ttcGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGt            <  2:1260110/97‑1 (MQ=255)

                                                                                            aaTCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTa       >  1:699915/1‑99 (MQ=255)

                                                                                            aaTCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTa       >  2:223524/1‑99 (MQ=255)

                                                                                             aTCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTaa      >  2:1023830/1‑99 (MQ=255)

                                                                                             aTCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTaa      >  1:656901/1‑99 (MQ=255)

GCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAG  >  NC_000913/366202‑366398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`