BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,337,109	A→C	11.6%	V771G (GTT→GGT)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,337,109	0	A	C	11.6%	333.1 / 27.5	103	V771G (GTT→GGT)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand): ref base A (43/48); new base C (9/3); total (52/51)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 107 total aligned reads displayed.

GCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGA  >  NC_000913/2337011‑2337207

gCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATaa                                                                                                    <  2:1174283/99‑1 (MQ=255)

gCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATaa                                                                                                    <  2:1482587/99‑1 (MQ=255)

  gTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAAcc                                                                                                  <  1:2250870/99‑1 (MQ=255)

   tACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAAccc                                                                                                 <  1:496206/99‑1 (MQ=255)

   tACCGGCATCGGTGATCAACATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAAccc                                                                                                 <  1:617327/99‑1 (MQ=255)

     ccGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCt                                                                                               <  1:1122839/99‑1 (MQ=255)

      cGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCtt                                                                                              <  1:2159643/99‑1 (MQ=255)

       ggCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCttt                                                                                             <  1:1403523/99‑1 (MQ=255)

         cATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcg                                                                                           <  1:732346/99‑1 (MQ=255)

           tCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcgtc                                                                                         >  1:919280/1‑99 (MQ=255)

           tCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcgtc                                                                                         <  1:1122449/99‑1 (MQ=255)

           tCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcgtc                                                                                         >  2:1518642/1‑99 (MQ=255)

              gTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCa                                                                                      <  2:2444051/99‑1 (MQ=255)

              gTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCa                                                                                      <  2:554215/99‑1 (MQ=255)

               tGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAc                                                                                     <  1:139448/99‑1 (MQ=255)

               tGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAc                                                                                     >  1:265120/1‑99 (MQ=255)

               tGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAc                                                                                     <  1:2070953/99‑1 (MQ=255)

               tGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAc                                                                                     >  2:264419/1‑99 (MQ=255)

                gATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcg                                                                                    <  1:127978/99‑1 (MQ=255)

                   catcatGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGa                                                                                 <  2:2075219/99‑1 (MQ=255)

                     tcatGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATACCCCCTTTCGTCGCACGCGACt                                                                               >  1:207663/1‑99 (MQ=255)

                     tcatGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACt                                                                               <  1:1318544/99‑1 (MQ=255)

                      catGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACtt                                                                              <  1:616901/99‑1 (MQ=255)

                       atGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttg                                                                             <  2:2027146/99‑1 (MQ=255)

                       atGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttg                                                                             <  1:373254/99‑1 (MQ=255)

                        tGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttgg                                                                            >  2:1630011/1‑99 (MQ=255)

                         gATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttggt                                                                           <  1:1802422/99‑1 (MQ=255)

                         gATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttggt                                                                           <  2:2233323/99‑1 (MQ=255)

                         gATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttggt                                                                           >  1:188299/1‑99 (MQ=255)

                            cTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttggttgg                                                                        <  1:1055289/99‑1 (MQ=255)

                             tGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATACCCCCTTTCGTCGCACGCGACTTGGTTggg                                                                       >  1:1807340/1‑99 (MQ=255)

                             tGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTggg                                                                       <  1:1632943/99‑1 (MQ=255)

                             tGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTggg                                                                       <  1:1122246/99‑1 (MQ=255)

                              ggTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGt                                                                      <  1:673966/99‑1 (MQ=255)

                                  gCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATACCCCCTTTCGTCGCACGCGACTTGGTTGGGTATTc                                                                  <  2:470410/99‑1 (MQ=255)

                                  gCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTc                                                                  <  2:2335991/99‑1 (MQ=255)

                                   cAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTcc                                                                 >  2:2503672/1‑99 (MQ=255)

                                    aGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCg                                                                >  2:2454158/1‑99 (MQ=255)

                                     gTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATACCCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGc                                                               >  2:121115/1‑99 (MQ=255)

                                     gTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGc                                                               >  1:1768591/1‑99 (MQ=255)

                                      tCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGcc                                                              >  2:209842/1‑99 (MQ=255)

                                      tCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGcc                                                              >  1:1553668/1‑99 (MQ=255)

                                      tCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGcc                                                              >  2:1598516/1‑99 (MQ=255)

                                        aTCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATACCCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCAc                                                            <  1:1266130/99‑1 (MQ=255)

                                        aTCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCAc                                                            <  2:1157762/99‑1 (MQ=255)

                                        aTCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCAc                                                            <  2:1566/99‑1 (MQ=255)

                                         tCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACt                                                           >  1:152510/1‑99 (MQ=255)

                                         tCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACt                                                           >  2:1346920/1‑99 (MQ=255)

                                          cTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTg                                                          >  2:1315444/1‑99 (MQ=255)

                                            aCCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCg                                                        >  2:467673/1‑99 (MQ=255)

                                             ccTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCgg                                                       <  1:1436715/99‑1 (MQ=255)

                                               tGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTa                                                     >  2:760803/1‑99 (MQ=255)

                                               tGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTa                                                     <  2:774986/99‑1 (MQ=255)

                                                gTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATACCCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTAc                                                    <  1:91410/99‑1 (MQ=255)

                                                 tACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTGTTCCGCCACTGCGGTACg                                                   >  2:869445/1‑99 (MQ=255)

                                                   ccGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGtt                                                 <  1:1425702/99‑1 (MQ=255)

                                                     gcgcCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTa                                               >  1:1011072/1‑99 (MQ=255)

                                                     gcgcCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTa                                               <  2:617625/99‑1 (MQ=255)

                                                      cgcCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTAc                                              >  1:1597274/1‑99 (MQ=255)

                                                        ccAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCg                                            <  2:1937265/99‑1 (MQ=255)

                                                         caacaaCTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGt                                           >  2:2033838/1‑99 (MQ=255)

                                                           acaacTAAACCGTTACGTTCGGTAACCTTGATGGAGATACCCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTaa                                         >  2:81558/1‑99 (MQ=255)

                                                               cTAAACCGTTACGTTCGGTAACCTTGATGGAGATACCCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGt                                     >  2:1505976/1‑99 (MQ=255)

                                                               cTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGt                                     <  1:540560/99‑1 (MQ=255)

                                                                tAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGtt                                    <  2:1494082/99‑1 (MQ=255)

                                                                 aaaCCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGttt                                   <  1:2138280/99‑1 (MQ=255)

                                                                 aaaCCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGttt                                   >  2:1327208/1‑99 (MQ=255)

                                                                 aaaCCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGttt                                   <  1:885083/99‑1 (MQ=255)

                                                                  aaCCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGtttt                                  >  2:1600782/1‑99 (MQ=255)

                                                                    ccGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTttgc                                >  1:1943129/1‑99 (MQ=255)

                                                                     cGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTttgcg                               <  2:743202/99‑1 (MQ=255)

                                                                         aCGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTttgcgttgc                           >  2:1003067/1‑99 (MQ=255)

                                                                          cGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTttgcgttgcg                          >  2:1149742/1‑99 (MQ=255)

                                                                           gTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCgg                         >  2:2484460/1‑99 (MQ=255)

                                                                            ttCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGt                        >  1:1047027/1‑99 (MQ=255)

                                                                            ttCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGt                        >  1:735421/1‑99 (MQ=255)

                                                                            ttCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGt                        <  1:353381/99‑1 (MQ=255)

                                                                            ttCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGt                        >  2:558295/1‑99 (MQ=255)

                                                                             tCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTg                       >  2:179716/1‑99 (MQ=255)

                                                                              cGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGa                      <  2:1793565/99‑1 (MQ=255)

                                                                                gTAACCTTGATGGAGATACCCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAgg                    >  2:519480/1‑99 (MQ=255)

                                                                                 tAACCTTGATGGAGATACCCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGa                   >  2:1565106/1‑99 (MQ=255)

                                                                                 tAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGa                   >  2:1147785/1‑99 (MQ=255)

                                                                                  aaCCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGAt                  >  1:1977120/1‑99 (MQ=255)

                                                                                   aCCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGAtt                 >  2:2514786/1‑99 (MQ=255)

                                                                                   aCCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGAtt                 <  2:359877/99‑1 (MQ=255)

                                                                                       tGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTgcgc             <  1:224478/99‑1 (MQ=255)

                                                                                         aTGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCa           <  2:120035/99‑1 (MQ=255)

                                                                                          tGGAGATACCCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCAt          >  2:2122013/1‑99 (MQ=255)

                                                                                          tGGAGATACCCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCAt          >  1:737206/1‑99 (MQ=255)

                                                                                          tGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCAt          >  2:2505185/1‑99 (MQ=255)

                                                                                          tGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCAt          >  2:1593830/1‑99 (MQ=255)

                                                                                             agaTAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGc       >  1:1646525/1‑99 (MQ=255)

                                                                                             agaTAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGc       >  2:1334424/1‑99 (MQ=255)

                                                                                              gaTAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGcc      >  2:2040144/1‑99 (MQ=255)

                                                                                               aTAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATTGCCa     <  1:2471936/99‑1 (MQ=255)

                                                                                               aTAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCa     <  2:1533375/99‑1 (MQ=255)

                                                                                                tAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCAc    >  1:983932/1‑99 (MQ=255)

                                                                                                 aaCCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACg   >  2:615357/1‑99 (MQ=255)

                                                                                                  aCCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGa  <  1:1634258/99‑1 (MQ=255)

GCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGA  >  NC_000913/2337011‑2337207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`