| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,535,147 | A→C | 100% | V241G (GTA→GGA) | envZ ← | sensory histidine kinase EnvZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,535,147 | 0 | A | C | 97.5% | 95.3 / ‑4.3 | 39 | V241G (GTA→GGA) | envZ | sensory histidine kinase EnvZ |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (23/15); total (23/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAA > NC_000913/3535053‑3535239 | tttATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCcccc < 2:1529804/99‑1 (MQ=255) aTTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGc > 2:724188/1‑99 (MQ=255) ttGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGcc > 2:996698/1‑99 (MQ=255) tGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCa > 1:2028608/1‑99 (MQ=255) gATCGATTCTGCCAGATAGCCACCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCAt > 1:846045/1‑99 (MQ=255) aTCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATc > 2:1847246/1‑99 (MQ=255) gATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATcagc > 2:2303145/1‑99 (MQ=255) ttCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATcagcag > 2:1061216/1‑99 (MQ=255) ttCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATcagcag < 1:2853546/99‑1 (MQ=255) cTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCg > 2:3015402/1‑99 (MQ=255) cTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCg < 1:801250/99‑1 (MQ=255) cAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCg < 1:1026960/99‑1 (MQ=255) gATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGt > 1:2880864/1‑99 (MQ=255) gATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGt > 1:172681/1‑99 (MQ=255) tAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCa < 2:1989638/99‑1 (MQ=255) tAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCa < 2:2004481/99‑1 (MQ=255) tCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGcc > 1:2854469/1‑99 (MQ=255) tGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCAGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGt < 2:1024899/99‑1 (MQ=255) tcatcTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAacac > 1:1494023/1‑99 (MQ=255) tcatcTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAacac > 1:1600164/1‑99 (MQ=255) tcatcTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAacac > 2:3035421/1‑99 (MQ=255) tctcAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAg > 1:1250630/1‑99 (MQ=255) tctcAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAg > 1:2836903/1‑99 (MQ=255) gTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAgccgcc > 2:2201759/1‑99 (MQ=255) tCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCa < 2:238738/99‑1 (MQ=255) gCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCata < 2:2364075/99‑1 (MQ=255) ccAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCatat < 2:2885052/99‑1 (MQ=255) cAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATg > 1:1738592/1‑99 (MQ=255) ggCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGTCGCCATATGGt > 2:1812215/1‑99 (MQ=255) gCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGtt < 2:1811167/99‑1 (MQ=255) cGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTa < 1:2441158/99‑1 (MQ=255) tCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACggg > 2:59980/1‑99 (MQ=255) gcggcgTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTaa > 1:1808495/1‑99 (MQ=255) gcggcgTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTaa < 1:1494721/99‑1 (MQ=255) gTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGaa < 1:1958222/99‑1 (MQ=255) tGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAAc > 2:1870764/1‑99 (MQ=255) tGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAAc > 2:801230/1‑99 (MQ=255) aaGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCAcc < 2:550433/99‑1 (MQ=255) tGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGaa < 1:1352271/99‑1 (MQ=255) | TTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAA > NC_000913/3535053‑3535239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |