BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	366,290	C→T	84.9%	D6N (GAT→AAT)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,290	0	C	T	84.9%	2647.1 / inf	881	D6N (GAT→AAT)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand): ref base C (64/69); new base T (391/357); total (455/426)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 886 total aligned reads displayed.

ACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGT  >  NC_000913/366192‑366388

   gcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGt                                                                                                 <  2:1514321‑M2/8‑1 (MQ=255)

   gcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGt                                                                                                 <  1:3031413‑M2/8‑1 (MQ=255)

     accggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTaa                                                                                               <  1:2701906‑M2/10‑1 (MQ=255)

       cTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATc                                                                                             <  2:1108599/99‑1 (MQ=255)

        ggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCa                                                                                            <  2:40312‑M2/13‑1 (MQ=255)

         gggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAt                                                                                           >  1:2646449‑M2/86‑99 (MQ=255)

          ggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCATg                                                                                          >  2:689590‑M2/85‑99 (MQ=255)

          ggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCATg                                                                                          >  1:1959720‑M2/85‑99 (MQ=255)

           gccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAtgt                                                                                         <  1:314258‑M2/16‑2 (MQ=255)

            gAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGt                                                                                        <  1:713374/99‑1 (MQ=255)

            ccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCatgtc                                                                                        <  1:886071‑M2/17‑3 (MQ=255)

            ccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCatgtc                                                                                        >  1:2752243‑M2/83‑97 (MQ=255)

             cagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATcatgtca                                                                                       <  2:2171158‑M2/18‑4 (MQ=255)

              agcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAAtcatgtcat                                                                                      >  2:1628725‑M2/81‑95 (MQ=255)

              agcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAAtcatgtcat                                                                                      <  2:2707698‑M2/19‑5 (MQ=255)

              agcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAAtcatgtcat                                                                                      >  1:1464476‑M2/81‑95 (MQ=255)

               gcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATa                                                                                    <  2:2417369‑M2/20‑1 (MQ=255)

                gggggATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATa                                                                                    <  2:579138/99‑1 (MQ=255)

                 ccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGc                                                                                  <  2:2682814‑M2/22‑1 (MQ=255)

                  gggATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGc                                                                                  >  1:1293506/1‑99 (MQ=255)

                  cgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCt                                                                                 <  2:549112‑M2/23‑1 (MQ=255)

                  cgagcgagttccgtgccggttgagaagaaaaagtgaatgatgtagccgtcaagtggtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCt                                                                                 >  2:280685‑M2/77‑99 (MQ=255)

                   gagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTg                                                                                <  1:1856479‑M2/24‑1 (MQ=255)

                   gagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTg                                                                                >  2:2231019‑M2/76‑99 (MQ=255)

                    gATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTg                                                                                >  1:1418190/1‑99 (MQ=255)

                    agcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGt                                                                               <  1:1999690‑M2/25‑1 (MQ=255)

                    agcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGt                                                                               >  1:2599428‑M2/75‑99 (MQ=255)

                         gttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCt                                                                          >  2:2887033‑M2/70‑99 (MQ=255)

                          ttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCtg                                                                         >  2:1201266‑M2/69‑99 (MQ=255)

                          ttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCtg                                                                         >  1:376971‑M2/69‑99 (MQ=255)

                             cgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCtgtgt                                                                      <  1:2081877‑M2/34‑1 (MQ=255)

                              aaGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCtgtgt                                                                      <  1:2266921/99‑1 (MQ=255)

                               tgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGa                                                                    <  2:1740401‑M2/36‑1 (MQ=255)

                               aGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCtgtgtg                                                                     >  1:2871334/1‑99 (MQ=255)

                                gccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGaa                                                                   >  1:656904‑M2/63‑99 (MQ=255)

                                 gCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGaa                                                                   >  1:2040173/1‑99 (MQ=255)

                                 ccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGaaa                                                                  <  1:1777522‑M2/38‑1 (MQ=255)

                                 ccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGaaa                                                                  <  1:1555530‑M2/38‑1 (MQ=255)

                                   ggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAAtt                                                                <  1:2204336‑M2/40‑1 (MQ=255)

                                     ttgtgaaggaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTTAAATTGt                                                              <  2:815637‑M2/42‑1 (MQ=255)

                                     ttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGt                                                              <  1:2980925‑M2/42‑1 (MQ=255)

                                      tgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGtt                                                             >  1:1630643‑M2/57‑99 (MQ=255)

                                      tAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGt                                                              <  1:150354/99‑1 (MQ=255)

                                        tgaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTAt                                                           >  1:1001491‑M2/55‑99 (MQ=255)

                                         gaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATc                                                          <  1:2447791‑M2/46‑1 (MQ=255)

                                         gaagaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATc                                                          <  2:1159835‑M2/46‑1 (MQ=255)

                                           agaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCg                                                        >  2:1247220‑M2/52‑99 (MQ=255)

                                            gaaaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGc                                                       >  1:1108868‑M2/51‑99 (MQ=255)

                                              aaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTc                                                     >  2:2136034‑M2/49‑99 (MQ=255)

                                              aaaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTc                                                     >  1:1366315‑M2/49‑99 (MQ=255)

                                               aaagtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTca                                                    >  2:2365988‑M2/48‑99 (MQ=255)

                                                  gtgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACaa                                                 <  2:2748138‑M2/55‑1 (MQ=255)

                                                   tgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAt                                                >  2:2554533‑M2/44‑99 (MQ=255)

                                                   tgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAt                                                >  1:497819‑M2/44‑99 (MQ=255)

                                                   tgaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAt                                                >  1:2395350‑M2/44‑99 (MQ=255)

                                                    gaatgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAtt                                               <  2:62400‑M2/57‑1 (MQ=255)

                                                      atgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTcc                                             >  1:1717315‑M2/41‑99 (MQ=255)

                                                      atgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTcc                                             >  2:1358286‑M2/41‑99 (MQ=255)

                                                       tgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCca                                            >  2:2305480‑M2/40‑99 (MQ=255)

                                                       tgatgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCca                                            >  2:36782‑M2/40‑99 (MQ=255)

                                                        ggTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCca                                            >  1:43006/1‑99 (MQ=255)

                                                         atgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcaca                                          <  1:2921470‑M2/62‑1 (MQ=255)

                                                          tgtagccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcacac                                         >  1:1044903‑M2/37‑99 (MQ=255)

                                                             agccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACacaac                                      >  2:147121‑M2/34‑99 (MQ=255)

                                                             agccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACacaac                                      <  2:2977245‑M2/66‑1 (MQ=255)

                                                              gccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACacaaca                                     <  1:1385455‑M2/67‑1 (MQ=255)

                                                              gccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACacaaca                                     <  2:2270708‑M2/67‑1 (MQ=255)

                                                               ccgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACAt                                    >  2:861277‑M2/32‑99 (MQ=255)

                                                                cgtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATa                                   >  1:550709‑M2/31‑99 (MQ=255)

                                                                 gtcaagttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATAc                                  <  2:1568576‑M2/70‑1 (MQ=255)

                                                                     agttgtcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGc                              >  2:2495119‑M2/26‑99 (MQ=255)

                                                                     acgacgTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAg                               >  1:2534163/1‑99 (MQ=255)

                                                                      cgacgTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGc                              >  2:1813438/1‑99 (MQ=255)

                                                                          tcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGaa                         <  1:1331230‑M2/79‑1 (MQ=255)

                                                                          tcataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGaa                         <  1:936218‑M2/79‑1 (MQ=255)

                                                                           cataattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAg                        <  2:516755‑M2/80‑1 (MQ=255)

                                                                             taattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCa                      <  2:141220‑M2/82‑1 (MQ=255)

                                                                             taattggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCa                      >  2:2003396‑M2/18‑99 (MQ=255)

                                                                                ttggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATaa                   >  1:749003‑M2/15‑99 (MQ=255)

                                                                                ttggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATaa                   >  2:1039371‑M2/15‑99 (MQ=255)

                                                                                ttggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATaa                   <  2:1282322‑M2/85‑1 (MQ=255)

                                                                                 tggtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATaaa                  <  1:832086‑M2/86‑1 (MQ=255)

                                                                                  acgacgGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATaaa                  >  1:1910683/1‑99 (MQ=255)

                                                                                   gtaccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAgt                <  1:2349946‑M2/88‑1 (MQ=255)

                                                                                      cgGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAgtgt              <  2:3033876/99‑1 (MQ=255)

                                                                                      ccgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTa             <  2:1897799‑M2/91‑1 (MQ=255)

                                                                                       cgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTaa            >  2:2456942‑M2/8‑99 (MQ=255)

                                                                                       cgagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTaa            <  1:2110436‑M2/92‑1 (MQ=255)

                                                                                        gagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTaaa           >  2:75349‑M2/7‑99 (MQ=255)

                                                                                        gagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTaaa           <  1:2814833‑M2/93‑1 (MQ=255)

                                                                                        gagctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTaaa           >  2:906816‑M2/7‑99 (MQ=255)

                                                                                         agctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAg          <  2:1703772‑M2/94‑1 (MQ=255)

                                                                                          gctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGc         >  1:2160520‑M2/5‑99 (MQ=255)

                                                                                          gctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGc         <  2:2089507‑M2/95‑1 (MQ=255)

                                                                                          gctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGc         <  2:1951149‑M2/95‑1 (MQ=255)

                                                                                           ctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGcc        <  1:603599‑M2/96‑1 (MQ=255)

                                                                                           ctcGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGcc        <  2:2607113‑M2/96‑1 (MQ=255)

                                                                                             cGAATTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTg      <  1:125010‑M2/98‑1 (MQ=255)

                                                                                              gAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTg      >  1:1193481/1‑99 (MQ=255)

                                                                                               aaTTCGTAATCAT‑GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTggg    >  1:681793/1‑99 (MQ=255)

ACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGT  >  NC_000913/366192‑366388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`