BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	401,284	G→T	35.4%	intergenic (‑361/‑102)	ddlA ← / → iraP	D‑alanine‑‑D‑alanine ligase A/anti‑adaptor protein for sigma(S) stabilization

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	401,284	0	G	T	35.4%	316.7 / 230.8	175	intergenic (‑361/‑102)	ddlA/iraP	D‑alanine‑‑D‑alanine ligase A/anti‑adaptor protein for sigma(S) stabilization
Reads supporting (aligned to +/- strand): ref base G (60/53); new base T (26/36); total (86/89)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

Only 100 of 182 total aligned reads displayed.

TTTATAATAAAGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACA  >  NC_000913/401188‑401382

tttatAATAAAGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTgcg                                                                                                  >  2:2394049/1‑99 (MQ=255)

tttatAATAAAGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTgcg                                                                                                  >  1:938476/1‑99 (MQ=255)

tttatAATAAAGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTgcg                                                                                                  <  1:2603101/99‑1 (MQ=255)

 ttatAATAAAGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTgcgc                                                                                                 <  2:3525062/99‑1 (MQ=255)

  tataATAAAGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCa                                                                                                >  2:2736356/1‑99 (MQ=255)

   ataataAAGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCaa                                                                                               >  1:2534700/1‑99 (MQ=255)

    taataaAGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCaaa                                                                                              >  2:1669716/1‑99 (MQ=255)

    taataaAGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCaaa                                                                                              <  1:3331366/99‑1 (MQ=255)

      ataaAGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGt                                                                                            >  1:755450/1‑99 (MQ=255)

      ataaAGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGt                                                                                            >  2:1226956/1‑99 (MQ=255)

       taaAGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTa                                                                                           >  2:2486737/1‑99 (MQ=255)

        aaaGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTAt                                                                                          <  2:896336/99‑1 (MQ=255)

          aGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTAttt                                                                                        <  2:1759241/99‑1 (MQ=255)

          aGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTAttt                                                                                        <  2:2541492/99‑1 (MQ=255)

           ggTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTc                                                                                       <  2:3438832/99‑1 (MQ=255)

           ggTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTc                                                                                       >  2:1288219/1‑99 (MQ=255)

              tGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCtt                                                                                    <  1:826236/99‑1 (MQ=255)

               gATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCttt                                                                                   >  2:212987/1‑99 (MQ=255)

               gATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCttt                                                                                   <  2:156642/99‑1 (MQ=255)

                aTAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTg                                                                                  <  1:3047939/99‑1 (MQ=255)

                aTAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTg                                                                                  >  1:2099707/1‑99 (MQ=255)

                 tAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGt                                                                                 <  1:3200771/99‑1 (MQ=255)

                 tAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGt                                                                                 <  1:95742/99‑1 (MQ=255)

                   aTTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCa                                                                               >  1:419517/1‑99 (MQ=255)

                    ttAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCAt                                                                              <  1:3179100/99‑1 (MQ=255)

                    ttAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCAt                                                                              >  1:2447347/1‑99 (MQ=255)

                    ttAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCAt                                                                              >  2:2195803/1‑99 (MQ=255)

                    ttAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCAt                                                                              <  1:3402024/99‑1 (MQ=255)

                     tAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATa                                                                             >  1:1163089/1‑99 (MQ=255)

                     tAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATa                                                                             >  2:3293461/1‑99 (MQ=255)

                       aaaGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCGTaaa                                                                           <  1:1538924/99‑1 (MQ=255)

                        aaGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATaaaa                                                                          >  1:2670466/1‑99 (MQ=255)

                         aGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATaaaaa                                                                         >  2:2453456/1‑99 (MQ=255)

                          gCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAaat                                                                        >  1:1842042/1‑99 (MQ=255)

                           ccTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAaata                                                                       <  1:3107056/99‑1 (MQ=255)

                           ccTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAaata                                                                       <  2:2273446/99‑1 (MQ=255)

                            cTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAaataa                                                                      <  1:1083440/99‑1 (MQ=255)

                            cTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAaataa                                                                      <  1:281564/99‑1 (MQ=255)

                              atatTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAataata                                                                    <  2:2554817/99‑1 (MQ=255)

                               tatTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATAc                                                                   <  2:3097744/99‑1 (MQ=255)

                                 ttttGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACtt                                                                 >  2:156179/1‑99 (MQ=255)

                                    tgtgtgGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCa                                                              >  1:2180652/1‑99 (MQ=255)

                                      tgtgGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGa                                                            >  2:565715/1‑99 (MQ=255)

                                      tgtgGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGa                                                            >  1:2911102/1‑99 (MQ=255)

                                       gtgGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGac                                                           <  2:3050294/99‑1 (MQ=255)

                                        tgGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGaca                                                          <  1:458984/99‑1 (MQ=255)

                                        tgGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGaca                                                          <  1:2213807/99‑1 (MQ=255)

                                         gggTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGacac                                                         <  1:2339272/99‑1 (MQ=255)

                                          ggTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACt                                                        <  2:1308515/99‑1 (MQ=255)

                                           gTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTa                                                       <  2:2741129/99‑1 (MQ=255)

                                           gTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTa                                                       <  2:1616619/99‑1 (MQ=255)

                                              attattTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGa                                                    >  1:1974297/1‑99 (MQ=255)

                                               ttattTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGaa                                                   >  2:2970908/1‑99 (MQ=255)

                                                tattTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAg                                                  <  2:1908545/99‑1 (MQ=255)

                                                  tttAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGtt                                                <  1:3285744/99‑1 (MQ=255)

                                                   ttAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTtg                                               >  1:2160031/1‑99 (MQ=255)

                                                      aaTAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTtgtgg                                            <  1:1937525/99‑2 (MQ=255)

                                                      aaTAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGa                                            >  1:2633237/1‑99 (MQ=255)

                                                      aaTAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGa                                            >  2:3016592/1‑99 (MQ=255)

                                                       aTAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGaa                                           >  2:1374447/1‑99 (MQ=255)

                                                        tAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGaaa                                          >  1:1698607/1‑99 (MQ=255)

                                                        tAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGaaa                                          >  2:1095078/1‑99 (MQ=255)

                                                          agagAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCACAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACa                                        >  1:1369141/1‑99 (MQ=255)

                                                            agaAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATa                                      >  1:1277405/1‑99 (MQ=255)

                                                             gaAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATaa                                     <  2:2449058/99‑1 (MQ=255)

                                                                aCGTTTCGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGt                                  <  2:1641330/99‑1 (MQ=255)

                                                                aCGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGt                                  <  2:2584543/99‑1 (MQ=255)

                                                                   tttCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTaa                               <  2:3029390/99‑1 (MQ=255)

                                                                    ttCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAAc                              <  2:1917863/99‑1 (MQ=255)

                                                                      cGCTGGTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACtt                            <  1:1587483/99‑1 (MQ=255)

                                                                      cGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACtt                            >  2:470504/1‑99 (MQ=255)

                                                                       gCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTtc                           <  1:1870264/99‑1 (MQ=255)

                                                                       gCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTtc                           >  2:2374580/1‑99 (MQ=255)

                                                                         tGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTtctc                         >  2:1052828/1‑99 (MQ=255)

                                                                         tGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTtctc                         <  1:1408498/99‑1 (MQ=255)

                                                                           gTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCa                       >  1:3023879/1‑99 (MQ=255)

                                                                           gTAATCAAACAAAAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCa                       >  1:2963128/1‑99 (MQ=255)

                                                                             aaTCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATa                     <  1:1235187/99‑1 (MQ=255)

                                                                             aaTCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATa                     >  2:2255218/1‑99 (MQ=255)

                                                                              aTCAAACAAAAAATATTTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATa                     <  1:2723164/99‑1 (MQ=255)

                                                                              aTCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATAc                    <  2:2627472/99‑1 (MQ=255)

                                                                               tCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACt                   >  1:1317149/1‑99 (MQ=255)

                                                                                caaacaaaAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACtt                  <  2:1691486/99‑1 (MQ=255)

                                                                                 aaacaaaAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACttt                 <  1:1510552/99‑1 (MQ=255)

                                                                                  aacaaaAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTg                >  1:3119204/1‑99 (MQ=255)

                                                                                   acaaaAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTgg               >  1:1691319/1‑99 (MQ=255)

                                                                                    caaaAAATA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGa              <  2:3241517/99‑1 (MQ=255)

                                                                                     aaaaaaTA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGAt             <  1:372607/99‑1 (MQ=255)

                                                                                     aaaaaaTA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGAt             <  2:732170/99‑1 (MQ=255)

                                                                                      aaaaaTA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATa            >  1:899312/1‑99 (MQ=255)

                                                                                       aaaaTA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATaa           >  2:1883520/1‑99 (MQ=255)

                                                                                       aaaaTA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATaa           <  1:2769066/99‑1 (MQ=255)

                                                                                       aaaaTA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATaa           >  1:192036/1‑99 (MQ=255)

                                                                                        aaaTA‑TTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAg          >  1:1091997/1‑99 (MQ=255)

                                                                                           ta‑tTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGaa       >  1:669674/1‑99 (MQ=255)

                                                                                           ta‑tTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGaa       >  1:2080623/1‑99 (MQ=255)

                                                                                           ta‑tTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGaa       <  2:2434568/99‑1 (MQ=255)

                                                                                            a‑tTTTCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGaaa      <  2:2003815/99‑1 (MQ=255)

                                                                                              tttGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAAt     >  2:3518797/1‑99 (MQ=255)

                                                                                                 gcgcAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACa  <  2:2824174/99‑1 (MQ=255)

TTTATAATAAAGGTTGATAATTAAAAGCCTATATTTTGTGTGGGTAATTATTTAAATAAGAGAAACGTTTCGCTGGTAATCAAACAAAAAATA‑TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACA  >  NC_000913/401188‑401382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`