Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	1,292,151	IS4 (+) +12 bp	7.9%	coding (695‑706/909 nt)	galU →	UTP‑‑glucose‑1‑phosphate uridylyltransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1292151 =	76 (0.960)	8 (0.090)	8/174	NT	8.4%	coding (695/909 nt)	galU	UTP‑‑glucose‑1‑phosphate uridylyltransferase
	?	NC_000913	4502090 =	NA (NA)					noncoding (1426/1426 nt)	IS4	repeat region
*	?	NC_000913	= 1292162	76 (0.960)	5 (0.050)	5/176	NT	5.4%	coding (706/909 nt)	galU	UTP‑‑glucose‑1‑phosphate uridylyltransferase
	?	NC_000913	= 4503515	NA (NA)					noncoding (1/1426 nt)	IS4	repeat region

CATGGCTCTTCCCTTTCATATGATAGGCTTCCACCGTTTCTTTTTCGATCAGCATATCAATTGCGTCGGTGAGCTGAATTTCATCACCAGCTCCCGGAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1292249‑1292151 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCC  >  NC_000913/4502090‑4502185                                                                                                                                                                                                     CATGGCTCTTCCCTTTCATATGATAGGCTTCCACCGTTTCTTTTTCGATCAGCATATCAATTGCGTCGGTGAGCTGAATTTCATCACCAGCTCCCGGAG                                                                                                  <  1:246452/99‑1                    ATGATAGGCTTCCACCGTTTCTTTTTCGATCAGCATATCAATTGCGTCGGTGAGCTGAATTTCATCACCAGCTCCCGGAGTAATGCCAGTCAGTTAAGC                                                                               <  1:1883293/99‑1                       ATAGGCTTCCACCGTTTCTTTTTCGATCAGCATATCAATTGCGTCGGTGAGCTGAATTTCATCACCAGCTCCCGGAGTAATGCCAGTCAGTTAAGCAAC                                                                            >  1:1864483/1‑99                        TAGGCTTCCACCGTTTCTTTTTCGATCAGCATATCAATTGCGTCGGTGAGCTGAATTTCATCACCAGCTCCCGGAGTAATGCCAGTCAGTTAAGCAACT                                                                           >  2:1393440/1‑99                           GCTTCCACCGTTTCTTTTTCGATCAGCATATCAATTGCGTCGGTGAGCTGAATTTCATCACCAGCTCCCGGAGTAATGCCAGTCAGTTAAGCAACTGAC                                                                        <  2:439425/99‑1                                                         TCAATTGCGTCGGTGAGCTGAATTTCATCACCAGCTCCCGGAGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTC                                          >  2:899435/1‑99                                                                     GTGAGCTGAATTTCATCACCAGCTCCCGGAGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCC                              <  2:618770/99‑1                                                                                TTCATCACCAGCTCCCGGAGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCT                   <  2:1734108/99‑1                                                                                          GCTCCCGGAGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCC         >  1:2127945/1‑99                                                                                                GGAGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCC   <  2:2303495/99‑1                                                                                                 GAGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCC  <  2:1479381/99‑1                                                                                                                                                                                                     CATGGCTCTTCCCTTTCATATGATAGGCTTCCACCGTTTCTTTTTCGATCAGCATATCAATTGCGTCGGTGAGCTGAATTTCATCACCAGCTCCCGGAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1292249‑1292151 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCC  >  NC_000913/4502090‑4502185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.