Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,619,235 C→T 77.4% T39I (ACC→ATC)  marR → DNA‑binding transcriptional repressor MarR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,619,2350CT77.4% 219.4 / 61.9 93T39I (ACC→ATC) marRDNA‑binding transcriptional repressor MarR
Reads supporting (aligned to +/- strand):  ref base C (10/11);  new base T (31/41);  total (41/52)
Fisher's exact test for biased strand distribution p-value = 8.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCAC  >  NC_000913/1619141‑1619330
                                                                                              |                                                                                               
ttCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATcgcg                                                                                             >  1:433987/1‑99 (MQ=255)
  cAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGc                                                                                           <  1:164471/99‑1 (MQ=255)
   aaTGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGca                                                                                          <  1:1801920/99‑1 (MQ=255)
   aaTGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGca                                                                                          >  2:1762528/1‑99 (MQ=255)
   aaTGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGca                                                                                          >  1:722218/1‑99 (MQ=255)
     tGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGcaca                                                                                        <  2:153519/99‑1 (MQ=255)
     tGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGcaca                                                                                        >  1:878799/1‑99 (MQ=255)
       aaaTTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGt                                                                                      <  1:2023520/99‑1 (MQ=255)
         attattCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGttt                                                                                    <  2:706353/99‑1 (MQ=255)
         attattCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGttt                                                                                    <  1:2033229/99‑1 (MQ=255)
         attattCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGttt                                                                                    >  2:601957/1‑99 (MQ=255)
          ttattCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTa                                                                                   >  1:1111500/1‑99 (MQ=255)
            attCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAg                                                                                 <  1:1869484/99‑1 (MQ=255)
             ttCCATTGGGTCGCATAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAgg                                                                                <  2:1559561/99‑1 (MQ=255)
                cATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGtgc                                                                             >  2:1059026/1‑99 (MQ=255)
                cATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGtgc                                                                             <  1:866290/99‑1 (MQ=255)
                 aTTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGtgct                                                                            >  1:418139/1‑99 (MQ=255)
                  ttGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGtgctc                                                                           <  2:972654/99‑1 (MQ=255)
                  ttGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGtgctc                                                                           <  2:441483/99‑1 (MQ=255)
                  ttGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGtgctc                                                                           >  1:974094/1‑99 (MQ=255)
                   tGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGtgctct                                                                          <  1:604174/99‑1 (MQ=255)
                      gTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGtgctctgct                                                                       >  1:1180632/1‑99 (MQ=255)
                       tCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGtgctctgctc                                                                      >  1:1200868/1‑99 (MQ=255)
                       tCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGtgctctgctc                                                                      <  1:829091/99‑1 (MQ=255)
                       tCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGtgctctgctc                                                                      <  2:274677/99‑1 (MQ=255)
                          cTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTAt                                                                   >  1:902560/1‑99 (MQ=255)
                           ttAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATc                                                                  <  2:2053692/99‑1 (MQ=255)
                              aTCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGc                                                               >  1:1938938/1‑99 (MQ=255)
                              aTCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGc                                                               >  2:399820/1‑99 (MQ=255)
                              aTCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGc                                                               <  2:78229/99‑1 (MQ=255)
                              aTCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGc                                                               >  1:2304182/1‑99 (MQ=255)
                               tCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCt                                                              >  1:1995600/1‑99 (MQ=255)
                               tCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCt                                                              >  2:1415924/1‑99 (MQ=255)
                                 cATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTgc                                                            >  2:326208/1‑99 (MQ=255)
                                   tatGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTgcgc                                                          >  1:1042670/1‑99 (MQ=255)
                                   tatGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTgcgc                                                          <  2:1822464/99‑1 (MQ=255)
                                   tatGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTgcgc                                                          >  1:1596457/1‑99 (MQ=255)
                                     tGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCgcgg                                                        >  2:2064686/1‑99 (MQ=255)
                                         tAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCgtg                                                    <  1:1638523/99‑1 (MQ=255)
                                          aaTCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCgtgt                                                   >  2:1445245/1‑99 (MQ=255)
                                           aTCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTa                                                  >  2:356587/1‑99 (MQ=255)
                                               gaagaaAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTAc                                              <  2:724571/99‑1 (MQ=255)
                                                 agaaagaTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTc                                            <  2:1641858/99‑1 (MQ=255)
                                                 agaaagaTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTc                                            >  1:488694/1‑99 (MQ=255)
                                                  gaaagaTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTcc                                           >  2:1295978/1‑99 (MQ=255)
                                                  gaaagaTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTcc                                           >  1:745957/1‑99 (MQ=255)
                                                   aaagaTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCg                                          >  1:301731/1‑99 (MQ=255)
                                                   aaagaTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCg                                          >  1:1076240/1‑99 (MQ=255)
                                                     agaTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGt                                        <  1:131884/99‑1 (MQ=255)
                                                     agaTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGt                                        >  2:501194/1‑99 (MQ=255)
                                                      gaTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGtt                                       <  1:936627/99‑1 (MQ=255)
                                                      gaTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGtt                                       <  2:2134490/99‑1 (MQ=255)
                                                       aTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTg                                      >  2:589373/1‑99 (MQ=255)
                                                       aTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTg                                      >  1:1561226/1‑99 (MQ=255)
                                                        tCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGa                                     >  1:1819123/1‑99 (MQ=255)
                                                        tCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGa                                     >  2:266113/1‑99 (MQ=255)
                                                         cGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGaa                                    <  1:1151302/99‑1 (MQ=255)
                                                          gCCTGCTTAACGGGTGTCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGGGGCGTGTATTACTCCGGTTGAAc                                   <  2:832032/99‑1 (MQ=255)
                                                              gCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGaa                               <  2:804448/99‑1 (MQ=255)
                                                              gCTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGaa                               >  2:1436630/1‑99 (MQ=255)
                                                               cTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGaaa                              <  1:1634779/99‑1 (MQ=255)
                                                               cTTAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGaaa                              <  2:801840/99‑1 (MQ=255)
                                                                ttAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGaaaa                             <  2:413474/99‑1 (MQ=255)
                                                                 tAACGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGaaaaa                            <  2:1216886/99‑1 (MQ=255)
                                                                  aaCGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAg                           <  1:1679534/99‑1 (MQ=255)
                                                                  aaCGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAg                           >  2:964772/1‑99 (MQ=255)
                                                                  aaCGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAg                           <  1:251496/99‑1 (MQ=255)
                                                                   aCGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAgg                          <  2:1925867/99‑1 (MQ=255)
                                                                    cGAGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGt                         <  1:113407/99‑1 (MQ=255)
                                                                     gAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTa                        <  1:1329246/99‑1 (MQ=255)
                                                                      aGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTAt                       <  1:1750086/99‑1 (MQ=255)
                                                                      aGTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTAt                       <  2:839875/99‑1 (MQ=255)
                                                                       gTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTAtt                      <  1:1407608/99‑1 (MQ=255)
                                                                       gTATCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTAtt                      >  2:694709/1‑99 (MQ=255)
                                                                         aTCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgt                    <  2:901485/99‑1 (MQ=255)
                                                                         aTCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgt                    <  1:1693112/99‑1 (MQ=255)
                                                                         aTCTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgt                    <  2:1920205/99‑1 (MQ=255)
                                                                           cTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgtcg                  <  1:2404959/99‑1 (MQ=255)
                                                                           cTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgtcg                  <  2:1826234/99‑1 (MQ=255)
                                                                           cTGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgtcg                  <  1:1208984/99‑1 (MQ=255)
                                                                            tGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgtcgg                 <  1:568850/99‑1 (MQ=255)
                                                                            tGTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgtcgg                 <  1:938640/99‑1 (MQ=255)
                                                                             gTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgtcggt                <  1:2154889/99‑1 (MQ=255)
                                                                             gTCTCCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgtcggt                <  1:1730929/99‑1 (MQ=255)
                                                                              tctcCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgtcggtc               <  2:1510976/99‑1 (MQ=255)
                                                                              tctcCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgtcggtc               <  1:1328289/99‑1 (MQ=255)
                                                                               ctcCGCTGGATATTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgtcggtcg              >  2:689580/1‑99 (MQ=255)
                                                                                       gATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGa      <  1:2200878/99‑1 (MQ=255)
                                                                                       gATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGa      >  1:1214357/1‑99 (MQ=255)
                                                                                        atatTATCGCGGCAGAGTATAAGGCACTCCGGGCTGTCCGCAGCGCGCCATGTATCAGTACGGTTGAAGTGAAAAAGGTAGTGTCGGTCGACCTGagag     >  2:2360898/1‑99 (MQ=255)
                                                                                        atatTATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAg     <  1:163020/99‑1 (MQ=255)
                                                                                         tattatCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGc    >  1:872117/1‑99 (MQ=255)
                                                                                           ttATCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCAc  >  1:1616797/1‑99 (MQ=255)
                                                                                           ttACCGCGGCACAGGTTGAGTTGCTCTGCTCTATCCGCGGCGCGGCGTGTATTACTGCGGTTGGACTGAGAAGGGTATTGGCGGTCGCCCTGGGTGCAc  >  2:776158/1‑99 (MQ=255)
                                                                                              |                                                                                               
TTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCAC  >  NC_000913/1619141‑1619330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑