Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,860,630	(C)5→4	coding (348/894 nt)	yidL →	putative DNA‑binding transcriptional regulator YidL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,860,626	0	C	.	100.0%	211.4 / NA	49	coding (344/894 nt)	yidL	putative DNA‑binding transcriptional regulator YidL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (31/18);  total (31/18)

AAGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTACCCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCG  >  NC_000913/3860488‑3860760                                                                                                                                           |                                                                                                                                       aaGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAcc                                                                                                                                       >  2:823362/1‑140 (MQ=255) aaGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAcc                                                                                                                                       >  2:567868/1‑140 (MQ=255) aaGGATGAGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAcc                                                                                                                                       >  2:97063/1‑140 (MQ=255)    gATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAcccca                                                                                                                                    <  2:776298/140‑2 (MQ=255)     aTGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAccccac                                                                                                                                   <  1:572391/140‑3 (MQ=255)     aTGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAccccac                                                                                                                                   <  1:520740/140‑3 (MQ=255)     aTGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAccccac                                                                                                                                   <  1:476473/140‑3 (MQ=255)      tgtgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAccccacc                                                                                                                                  >  2:1037759/1‑137 (MQ=255)              aaTTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTatgat                                                                                                                         <  1:1037759/140‑1 (MQ=255)              aaTTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTatgat                                                                                                                         <  1:750880/140‑1 (MQ=255)              aaTTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTatgat                                                                                                                         <  1:435610/140‑1 (MQ=255)              aaTTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTatgat                                                                                                                         <  1:428187/140‑1 (MQ=255)                         aGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCg                                                                                                              <  1:567868/140‑1 (MQ=255)                              ggCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGt                                                                                                         <  1:653835/140‑1 (MQ=255)                                        tAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAgtg                                                                                               <  2:135837/140‑3 (MQ=255)                                        tAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCg                                                                                               <  1:852846/140‑1 (MQ=255)                                               gCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGttattta                                                                                        >  1:350623/1‑140 (MQ=255)                                                cATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGttatttat                                                                                       >  2:815477/1‑140 (MQ=255)                                                        cTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCg                                                                                >  2:888562/1‑139 (MQ=255)                                                        cTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCg                                                                                <  1:888562/139‑1 (MQ=255)                                                                   tCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATc                                                                    >  2:622263/1‑140 (MQ=255)                                                                   tCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATc                                                                    >  2:140733/1‑140 (MQ=255)                                                                   tCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATc                                                                    >  1:975963/1‑140 (MQ=255)                                                                     ttAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAg                                                                  >  2:866351/1‑140 (MQ=255)                                                                     ttAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAg                                                                  >  2:435019/1‑140 (MQ=255)                                                                     ttAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAg                                                                  >  2:989177/1‑140 (MQ=255)                                                                       aaaGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGa                                                                >  2:574828/1‑140 (MQ=255)                                                                       aaaGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGa                                                                >  2:493428/1‑140 (MQ=255)                                                                       aaaGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGa                                                                >  2:678558/1‑140 (MQ=255)                                                                        aaGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGaa                                                               <  1:140733/140‑1 (MQ=255)                                                                        aaGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGaa                                                               <  2:282500/140‑1 (MQ=255)                                                                          gCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACt                                                             >  1:783789/1‑140 (MQ=255)                                                                          gCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACt                                                             >  1:204321/1‑140 (MQ=255)                                                                               tGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCg                                                        >  2:1009906/1‑140 (MQ=255)                                                                                     ttCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTg                                                  <  2:204321/140‑1 (MQ=255)                                                                                     ttCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTg                                                  <  2:975963/140‑1 (MQ=255)                                                                                        aCTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTg                                               <  2:350623/140‑1 (MQ=255)                                                                                            ttATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCAc                                                          >  2:53305/1‑125 (MQ=255)                                                                                            ttATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCAc                                                          <  1:53305/125‑1 (MQ=255)                                                                                             tATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTta                                          >  1:654926/1‑140 (MQ=255)                                                                                                  cTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAAc                                     <  1:1041503/140‑1 (MQ=255)                                                                                                   tgtgAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACt                                    >  2:662861/1‑140 (MQ=255)                                                                                                   tgtgAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACt                                    >  2:549875/1‑140 (MQ=255)                                                                                                    gtgAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACtt                                   >  2:544561/1‑140 (MQ=255)                                                                                                    gtgAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACtt                                   >  2:324362/1‑140 (MQ=255)                                                                                                    gtgAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACtt                                   >  1:237600/1‑140 (MQ=255)                                                                                                      gAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCa                                 >  1:767390/1‑140 (MQ=255)                                                                                                             tAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAg                          <  1:662861/140‑1 (MQ=255)                                                                                                                    ggAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATaaa                   <  1:324362/140‑1 (MQ=255)                                                                                                                          gTACTGGATGGAATTTA‑CCCCGCCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAaataa             >  2:740791/1‑140 (MQ=255)                                                                                                                           tACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAaataat            >  1:1039137/1‑140 (MQ=255)                                                                                                                              tggatggaATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTg         >  2:279472/1‑140 (MQ=255)                                                                                                                                   ggaATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGt    >  1:393533/1‑140 (MQ=255)                                                                                                                                     aaTTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCg  >  2:244173/1‑140 (MQ=255)                                                                                                                                     aaTTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCg  >  1:156366/1‑140 (MQ=255)                                                                                                                                     aaTTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCg  >  2:951475/1‑140 (MQ=255)                                                                                                                                     aaTTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCg  >  1:761063/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                       AAGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTACCCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCG  >  NC_000913/3860488‑3860760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑