Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,949,993	(T)5→6	coding (752/1332 nt)	argA →	N‑acetylglutamate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,949,988	1	.	T	100.0%	148.4 / NA	42	R249R (CGC→CGT)	argA	N‑acetylglutamate synthase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (24/18);  total (24/18)

AAAAGATGATTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑TTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAG  >  NC_000913/2949849‑2950122                                                                                                                                             |                                                                                                                                        aaaaGATGATTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTgcgc                                                                                                                                          >  1:734664/1‑140 (MQ=255)          ttGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑tttt‑ttgcg                                                                                                                               <  2:545007/140‑5 (MQ=255)               ttttGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGgcg                                                                                                                           <  2:817021/140‑1 (MQ=255)               ttttGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGgcg                                                                                                                           <  1:631823/140‑1 (MQ=255)                     tctTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGa                                                                                                                     <  2:737122/140‑1 (MQ=255)                              ggCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGcc                                                                                                            >  2:5791/1‑140 (MQ=255)                                   cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCTTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  2:34465/1‑140 (MQ=255)                                        aTGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgt                                                                                                  >  2:66243/1‑140 (MQ=255)                                         tGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtg                                                                                                 <  2:1038923/140‑1 (MQ=255)                                            cgacgGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtgcgt                                                                                              >  2:692273/1‑140 (MQ=255)                                                ggTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCt                                                                                          <  1:66243/140‑1 (MQ=255)                                                ggTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCt                                                                                          <  1:1022896/140‑1 (MQ=255)                                                ggTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCt                                                                                          <  1:135719/140‑1 (MQ=255)                                                ggTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCt                                                                                          >  2:135719/1‑140 (MQ=255)                                                  tGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGt                                                                                        >  2:309516/1‑140 (MQ=255)                                                     tatTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCAt                                                                                     <  1:153265/140‑1 (MQ=255)                                                          tctcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt                                                                                <  1:277251/140‑1 (MQ=255)                                                           ctcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATc                                                                               >  2:25807/1‑140 (MQ=255)                                                            tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCg                                                                                            <  1:546537/126‑1 (MQ=255)                                                            tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCg                                                                                            >  2:546537/1‑126 (MQ=255)                                                            tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCa                                                                              >  2:401538/1‑140 (MQ=255)                                                              cGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTaa                                                                                 <  1:597449/135‑1 (MQ=255)                                                              cGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTaa                                                                                 >  2:597449/1‑135 (MQ=255)                                                              cGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGt                                                                            >  2:407448/1‑140 (MQ=255)                                                                              gAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcg                                                            <  2:734664/140‑1 (MQ=255)                                                                                agcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcg                                                          <  1:25807/140‑1 (MQ=255)                                                                                 gcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc                                                         <  1:401538/140‑1 (MQ=255)                                                                                    caagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTGCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCGTTTAATCAGTTATCAGGAAGATGGCGCGCTGt                                                      >  2:209597/1‑140 (MQ=255)                                                                                      agcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTg                                                    >  1:1036643/1‑140 (MQ=255)                                                                                                   gCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTtct                                       >  1:20429/1‑140 (MQ=255)                                                                                                       aGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcg                                   >  1:277723/1‑140 (MQ=255)                                                                                                        ggAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgc                                  <  2:20429/140‑1 (MQ=255)                                                                                                         gAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgcg                                 >  1:194197/1‑140 (MQ=255)                                                                                                             agaAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACgg                             <  1:34465/140‑1 (MQ=255)                                                                                                                aaGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTAt                          <  2:194197/140‑1 (MQ=255)                                                                                                                  ggCGATTACAACTCCGGTACGGTGCGC‑TTTTATGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCg                        >  2:218715/1‑140 (MQ=255)                                                                                                                   gCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCgg                       <  2:277723/140‑1 (MQ=255)                                                                                                                      aTTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTAc                    >  2:547212/1‑140 (MQ=255)                                                                                                                         aCAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGCTGTTCTCACGCGACGGTATCGGTACGCa                 >  1:326937/1‑140 (MQ=255)                                                                                                                         aCAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCAGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCa                 >  1:919352/1‑140 (MQ=255)                                                                                                                                  gTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGAt        >  1:1042464/1‑140 (MQ=255)                                                                                                                                    aCGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGGACGCAGATTGTGATgg      >  2:935776/1‑140 (MQ=255)                                                                                                                                     cGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGa     >  1:839385/1‑140 (MQ=255)                                                                                                                                        tGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAg  >  2:15293/1‑140 (MQ=255)                                                                                                                                        tGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAg  >  1:958238/1‑140 (MQ=255)                                                                                                                                        tGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAg  >  2:852761/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                        AAAAGATGATTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑TTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAG  >  NC_000913/2949849‑2950122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑