Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,270,488	(C)7→8	noncoding (105/377 nt)	rnpB ←	RNase P catalytic RNA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,270,481	1	.	C	100.0%	91.4 / NA	26	noncoding (112/377 nt)	rnpB	RNase P catalytic RNA component
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (8/18);  total (8/18)

GAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGT‑CG‑T‑GGG‑TTT‑‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGC  >  NC_000913/3270356‑3270615                                                                                                                                   |                                                                                                                                         gAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTTTTTTACTGGT‑CC‑TGGGG‑TTT‑‑CCCCCCCCGGCGt                                                                                                                            >  2:176494/1‑140 (MQ=255) gAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGt                                                                                                                            <  2:287946/140‑1 (MQ=255)            gCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCAcc                                                                                                                 <  2:517472/140‑1 (MQ=255)                              ccGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCGGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCACTGGT‑CG‑TGGGG‑TTA‑‑CCCCCCCCCGCGTTACCTGGCCCCCCCCCCCAT‑GGAGGCCgg                                                                                              >  2:717968/1‑140 (MQ=255)                               cgcgcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTCTCCTTTTCTCCGGG‑CG‑TGGGG‑TTT‑‑CCCCCCCCCGCCCTTCCTCGCGCCCCGCCCCAT‑GGGGGCCggg                                                                                             >  2:1034043/1‑139 (MQ=255)                               cgcgcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGa                                                                                             <  2:982231/140‑1 (MQ=255)                                 cgcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCACTGGT‑CG‑TGGGG‑TTT‑‑CCCCCCCCGGCGTTACCTCGCACCCCCCCCCAT‑GGAGccccggct                                                                                           >  2:12071/1‑135 (MQ=255)                                 cgcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGCTTTCTCTCTCTTGCCCTGGT‑CGCG‑GGG‑TTT‑‑CCCCCCCAGGCCTTACCTCGCACCCCCCCCCATGGGAGCCCggcc                                                                                            >  2:216039/1‑138 (MQ=255)                                       gcgcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTtcctc                                                                                     <  1:717968/140‑1 (MQ=255)                                        cgcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGT‑CG‑TGGGG‑TTC‑‑CCCCCCCCGGCGTTACCTGGCACCCCCCCCCAT‑GGAGCCCGGGCTTtcctcc                                                                                    >  2:560970/1‑140 (MQ=255)                                                   cACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGt                                                                         <  1:216039/140‑1 (MQ=255)                                                     cccTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTTCACCGGTCCG‑G‑GGG‑TTC‑‑CCCCCCCCGGCGGTACCCCGCCCCCCCCCCCAT‑TGAGCCCCGGCTTTTCTCcccccccccccgcc                                                                       >  2:144837/1‑136 (MQ=255)                                                     cccTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGCACTGGT‑CG‑T‑GGG‑GTTCCCCCCCCCCCGCGGTACCCGGCACCCCCCCCCAT‑TGAGCCCCGGCTTTTCTCccccccccccccc                                                                        >  2:623690/1‑137 (MQ=255)                                                     cccTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGtct                                                                       <  1:394979/140‑1 (MQ=255)                                                        tttCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTccc                                                                    <  1:452209/140‑1 (MQ=255)                                                                        tCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCGCTGGT‑CC‑T‑GGGTTTT‑‑CCCCCCCCCGCCCTTCCTGGCCCCCCCCCCCCT‑GGGGCCCGGCCTTTTCTCCCCCCCCCCCCCCCCCCCCGAAGAGgacgacga                                                    >  2:6454/1‑140 (MQ=255)                                                                        tCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTTTTGCACTGGT‑CG‑TGGGG‑TTC‑‑CCCCCCCCGGCGGTACCTGACACCCCGCCCCTT‑GGGGCCCGGGCTTTTCCACCCCCCCCCCCCCTCCCCCCCAGAGggcgacga                                                    >  2:830780/1‑140 (MQ=255)                                                                                 cgcgGGCCATCGGCGGTTTTCTCTCTGTTTCACCGGG‑CGCG‑GGG‑TTT‑‑CCCCCCCCCGGGTTTCCCGGCCCCCCCCCCCAT‑GGGGCCCCGGCTTTCCCCCCCCCCCCCCCCCTCCCCCCAAGAGGGCGACGACGAAacggc                                           >  2:428087/1‑140 (MQ=255)                                                                                 cgcgGGCCATCGGCGGTTTTCTCTCTGTTGCACTGGG‑CG‑TGGGG‑TTT‑‑CCCCCCCCCGCGTTACCTGGCCCCCCCCCCCAT‑GGAGCCCCGGCTTTCCTCCCCCCCCCCCCCCTCCCCCCCCGAGGACGACGACGGAgcggg                                           >  2:483647/1‑139 (MQ=255)                                                                                                    gCTCTCTGTTGCACTGGT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCg                        <  1:387928/140‑1 (MQ=255)                                                                                                       ctctGTTGCACTGGT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcg                     <  1:176494/140‑1 (MQ=255)                                                                                                       ctctGTTGCACTGGT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcg                     <  1:428087/140‑1 (MQ=255)                                                                                                        tctGTTGCACTGGT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgc                    <  1:12071/140‑1 (MQ=255)                                                                                                         ctGTTGCACTGGT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcg                   <  1:560970/140‑1 (MQ=255)                                                                                                          tGTTGCACTGGT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc                  <  2:226639/140‑1 (MQ=255)                                                                                                             tGCACTGGT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc                  <  2:201087/137‑1 (MQ=255)                                                                                                             tGCACTGGT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc                  >  1:201087/1‑137 (MQ=255)                                                                                                                    gT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCtt                          >  2:440438/1‑122 (MQ=255)                                                                                                                    gT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCtt                          <  1:440438/122‑1 (MQ=255)                                                                                                                    gT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAgg        >  1:936809/1‑140 (MQ=255)                                                                                                                    gT‑CG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAgg        >  1:278736/1‑140 (MQ=255)                                                                                                                       cG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc                  <  1:811602/128‑1 (MQ=255)                                                                                                                       cG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc                  >  2:811602/1‑128 (MQ=255)                                                                                                                       cG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc                  <  2:815134/128‑1 (MQ=255)                                                                                                                       cG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc                  >  1:815134/1‑128 (MQ=255)                                                                                                                       cG‑T‑GGG‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGt      >  2:986591/1‑140 (MQ=255)                                                                                                                            ggg‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTg   >  1:667199/1‑140 (MQ=255)                                                                                                                             gg‑TTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTgc  <  1:830780/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         GAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGT‑CG‑T‑GGG‑TTT‑‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAT‑GGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGC  >  NC_000913/3270356‑3270615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑