| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,615,011 | (C)5→6 | coding (533/864 nt) | yjjW ← | putative glycyl‑radical enzyme activating enzyme YjjW |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,615,006 | 1 | . | C | 90.2% | 131.0 / 7.4 | 41 | coding (538/864 nt) | yjjW | putative glycyl‑radical enzyme activating enzyme YjjW |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base C (18/19); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
GTGTTGCAAATAATCCACCTGGCCAGGGATCACCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CT‑CC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAG > NC_000913/4614872‑4615140 | gtgtTGCAAATAATCCACCTGGCCAGGGATCACCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CT‑cc‑ccc > 1:25634/1‑140 (MQ=255) tgtTGCAAATAATCCACCTGGCCAGGGATCACCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CT‑cc‑cccc > 1:726052/1‑139 (MQ=255) gtTGCAAATAATCCACCTGGCCAGGGATCACCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CT‑cc‑cccca < 1:166101/140‑3 (MQ=255) gtTGCAAATAATCCACCTGGCCAGGGATCACCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CT‑cc‑cccca < 1:77312/140‑3 (MQ=255) tGCAAATAATCCACCTGGCCAGGGATCACCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CT‑cc‑ccccacg < 1:196799/140‑5 (MQ=255) ataatCCACCTGGCCAGGGATCACCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTg < 2:117461/140‑1 (MQ=255) atCCACCTGGCCAGGGATCACCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTgaga < 1:900684/140‑1 (MQ=255) tCCACCTGGCCAGGGATCACCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGAt > 2:61981/1‑140 (MQ=255) aCCTGGCCAGGGATCACCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGCTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGa > 1:671212/1‑140 (MQ=255) ccAGGGATCACCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTg < 2:371013/140‑1 (MQ=255) gggATCACCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTgcgc > 2:329170/1‑140 (MQ=255) ggATCACCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGcc > 1:829362/1‑140 (MQ=255) aCCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGc > 2:993894/1‑140 (MQ=255) aCCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGc > 2:455055/1‑140 (MQ=255) aCCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGc > 2:244997/1‑140 (MQ=255) aaCGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCgg > 1:193741/1‑140 (MQ=255) aCGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCggg > 1:423909/1‑140 (MQ=255) cgcgCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGt > 2:272847/1‑140 (MQ=255) gcgcTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGtt < 1:206134/140‑1 (MQ=255) ctctGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCg > 1:646145/1‑140 (MQ=255) aGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACa < 1:314292/140‑1 (MQ=255) aaaTAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGc < 2:423909/140‑1 (MQ=255) aTAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCGCCC‑CC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCg < 1:61981/140‑1 (MQ=255) tAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCGCCC‑CC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGt > 1:555915/1‑140 (MQ=255) tgctgcGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACt < 1:125134/140‑1 (MQ=255) gcTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTccac > 1:252476/1‑140 (MQ=255) cTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTccacc > 2:793994/1‑140 (MQ=255) tAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAg < 1:455055/140‑1 (MQ=255) tgATTATCGCGTCCGGTGAGTTGTTGATGACATTCGCCC‑CC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTga < 2:555915/140‑1 (MQ=255) cgcgTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCCCCCCGGGAGAAATTTTCCCCAGCCGGTTTCGCTCACCATGCCGTTACTGTCCACCAGACAGGTGAGATGgcg < 2:193741/140‑1 (MQ=255) cgcgTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGgcg > 1:295190/1‑140 (MQ=255) cgcgTCCGGTGAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGgcg < 2:829362/140‑1 (MQ=255) gAGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGa < 1:890345/140‑1 (MQ=255) aGTTGTTGATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGAt < 1:272847/140‑1 (MQ=255) gttgATGACATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGtt > 1:741345/1‑140 (MQ=255) atgaCATTCG‑CT‑CCTCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTg > 1:392304/1‑140 (MQ=255) tgaCATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGa < 1:993894/140‑1 (MQ=255) tgaCATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGa < 2:741345/140‑1 (MQ=255) tgaCATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGa < 1:17634/140‑1 (MQ=255) tgaCATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGa < 1:341041/140‑1 (MQ=255) cATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGa > 2:587906/1‑137 (MQ=255) cATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGa < 1:587906/137‑1 (MQ=255) cATTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAg < 1:244997/140‑1 (MQ=255) aTTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCg > 1:803330/1‑129 (MQ=255) aTTCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCg < 2:803330/129‑1 (MQ=255) tCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAg > 1:339311/1‑140 (MQ=255) tCG‑CTCCC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAg < 2:252476/140‑1 (MQ=255) | GTGTTGCAAATAATCCACCTGGCCAGGGATCACCAGCAAACGCAGTTCCGCCAGCTTGCCGCGCTCTGCCAGCAAATAGATGCTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCG‑CT‑CC‑CCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAG > NC_000913/4614872‑4615140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |