Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	766,957	(G)6→7	coding (974/1977 nt)	mngA →	2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	766,951	1	.	G	100.0%	141.1 / NA	39	A323G (GCG→GGG)	mngA	2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (18/21);  total (18/21)

AACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑GGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCT  >  NC_000913/766816‑767053                                                                                                                                         |                                                                                                       aacaaGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑gggg                                                                                                    >  1:205503/1‑140 (MQ=255)      gcgcTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑gggggggca                                                                                               <  1:107691/140‑4 (MQ=255)       cgcTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑gggggggcac                                                                                              >  1:56356/1‑136 (MQ=255)         cTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACgg                                                                                             >  2:195210/1‑140 (MQ=255)                   aGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGt                                                                                   <  2:205503/140‑1 (MQ=255)                       tAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTcgcg                                                                               >  2:1005193/1‑140 (MQ=255)                        aGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCgg                                                                              >  2:630004/1‑140 (MQ=255)                        aGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCgg                                                                              >  2:570946/1‑140 (MQ=255)                          cGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTa                                                                            >  2:300172/1‑140 (MQ=255)                            aTGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTAtt                                                                          >  1:800614/1‑140 (MQ=255)                              gagaCGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTAc                                                                        <  2:455676/140‑1 (MQ=255)                                 aCGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGt                                                                     >  1:397960/1‑140 (MQ=255)                                           tacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCtt                                                           <  1:867692/140‑1 (MQ=255)                                           tacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCtt                                                           <  1:300172/140‑1 (MQ=255)                                           tacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCtt                                                           >  2:867692/1‑140 (MQ=255)                                              aGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCgg                                                        <  1:97927/140‑1 (MQ=255)                                              aGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCg                                                         <  2:113469/139‑1 (MQ=255)                                              aGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCg                                                         >  1:113469/1‑139 (MQ=255)                                               gCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCggg                                                       <  1:570946/140‑1 (MQ=255)                                               gCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCggg                                                       >  1:322818/1‑140 (MQ=255)                                                   gATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTAc                                                   <  1:702644/140‑1 (MQ=255)                                                   gATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTAc                                                   <  1:467387/140‑1 (MQ=255)                                                   gATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTAc                                                   <  2:397960/140‑1 (MQ=255)                                                   gATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAAACTTCGGGCTAc                                                   >  1:512976/1‑140 (MQ=255)                                                     tACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACaa                                                 >  1:588698/1‑140 (MQ=255)                                                     tACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACaa                                                 >  1:917662/1‑140 (MQ=255)                                                        gCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt                                              <  2:940977/140‑1 (MQ=255)                                                        gCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt                                              >  1:248806/1‑140 (MQ=255)                                                             cGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt                                              <  1:624404/135‑1 (MQ=255)                                                             cGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt                                              >  2:624404/1‑135 (MQ=255)                                                                  aaaaGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATg                                    <  1:817664/140‑1 (MQ=255)                                                                         tCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGaaaa                             >  1:450121/1‑140 (MQ=255)                                                                            aaaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTc                          <  2:322818/140‑1 (MQ=255)                                                                            aaaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTc                          <  1:1005193/140‑1 (MQ=255)                                                                             aaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTcc                         <  1:857448/140‑1 (MQ=255)                                                                              aCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCt                        <  2:450121/140‑1 (MQ=255)                                                                              aCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCt                        <  2:485105/140‑1 (MQ=255)                                                                                 gAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGc                     <  2:56356/140‑1 (MQ=255)                                                                                    cTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCtgt                  <  1:195210/140‑1 (MQ=255)                                                                                                 tGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCaa     >  2:424592/1‑140 (MQ=255)                                                                                                   ttGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGc   >  1:411530/1‑140 (MQ=255)                                                                                                    tGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCt  <  1:424592/140‑1 (MQ=255)                                                                                                                                         |                                                                                                       AACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑GGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCT  >  NC_000913/766816‑767053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑