Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,066,904	(A)6→7	coding (188/228 nt)	yccJ ←	PF13993 family protein YccJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,066,898	1	.	A	100.0%	100.9 / NA	29	W65L (TGG→TTG)	yccJ	PF13993 family protein YccJ
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (14/15);  total (14/15)

GATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCC‑AAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGT  >  NC_000913/1066770‑1067034                                                                                                                                  |                                                                                                                                         gATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCC‑aaaaaaagcga                                                                                                                               <  1:891744/140‑6 (MQ=255)   tGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAAt                                                                                                                              <  1:172854/140‑1 (MQ=255)   tGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAAt                                                                                                                              >  2:792128/1‑140 (MQ=255)        tCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTa                                                                                                                         >  1:834607/1‑140 (MQ=255)         cTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTAt                                                                                                                        <  1:463731/140‑1 (MQ=255)            cccGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCgg                                                                                                                     >  2:397410/1‑140 (MQ=255)            cccGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCgg                                                                                                                     >  1:62802/1‑140 (MQ=255)                    aTAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCa                                                                                                             <  1:486262/140‑1 (MQ=255)                    aTAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCa                                                                                                             <  1:792128/140‑1 (MQ=255)                           tGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCtt                                                                                                      >  2:245951/1‑140 (MQ=255)                               aGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGAc                                                                                                  <  2:964854/140‑1 (MQ=255)                                  gtgtTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTaa                                                                                               <  1:245951/140‑1 (MQ=255)                                  gtgtTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGACTTAGCAAAGGCTTTGACTaa                                                                                               <  2:473351/140‑1 (MQ=255)                                     ttGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAacac                                                                                            >  1:515882/1‑140 (MQ=255)                                             aGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCAc                                                                                    >  2:489832/1‑140 (MQ=255)                                                   gAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACctt                                                                              <  2:834607/140‑1 (MQ=255)                                                              aaaCTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATc                                                                   >  1:1003347/1‑140 (MQ=255)                                                               aaCTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCt                                                                  >  1:1001277/1‑140 (MQ=255)                                                                  tCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCtttt                                                               >  2:166663/1‑140 (MQ=255)                                                                    ccccGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTcc                                                             <  1:394445/140‑1 (MQ=255)                                                                       cgcgAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGcc                                                          >  1:184049/1‑140 (MQ=255)                                                                           agaAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCAttt                                                      <  2:62802/140‑1 (MQ=255)                                                                                   gggAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTtcgtcg                                              <  2:515882/140‑1 (MQ=255)                                                                                        tCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTAccc                                         >  2:876698/1‑140 (MQ=255)                                                                                            tGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCg                                     <  1:876698/140‑1 (MQ=255)                                                                                            tGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCg                                     <  1:489832/140‑1 (MQ=255)                                                                                                        tttACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCACCATTTTTTCGTCGTACCCGGCGACTTCGAAAATg                         >  2:354275/1‑140 (MQ=255)                                                                                                          tACGTTCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGc                       <  1:1042692/140‑1 (MQ=255)                                                                                                               tCGATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCgg                  <  1:68506/140‑1 (MQ=255)                                                                                                                 gATGGTCTGTTCGCCCCAAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCt                >  1:919244/1‑140 (MQ=255)                                                                                                                              ccca‑AAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGt  >  2:801246/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                         GATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCC‑AAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGT  >  NC_000913/1066770‑1067034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑