Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,097,037	(G)7→8	intergenic (+208/+528)	ycdU → / ← serX	uncharacterized protein YcdU/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,030	1	.	G	100.0%	67.7 / NA	24	intergenic (+201/+535)	ycdU/serX	uncharacterized protein YcdU/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (0/24);  total (0/24)

TGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑ACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGC  >  NC_000913/1096895‑1097153                                                                                                                                         |                                                                                                                             tgACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑gggg                                                                                                                          <  2:353595/140‑1 (MQ=255)        gTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggggggggatt                                                                                                                   >  2:1008223/1‑136 (MQ=255)              aGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcg                                                                                                              <  2:37632/140‑1 (MQ=255)               gACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGGCtcgc                                                                                                             >  1:899294/1‑140 (MQ=255)               gACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgc                                                                                                             <  2:572217/140‑1 (MQ=255)                 cAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGACtcgctt                                                                                                           >  1:53721/1‑140 (MQ=255)                     tGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgcttcgct                                                                                                       <  1:621803/140‑1 (MQ=255)                       aTGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTtcgctcg                                                                                                     >  1:955186/1‑140 (MQ=255)                          tgCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGccc                                                                                                  <  1:858701/140‑1 (MQ=255)                               gATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCgg                                                                                             <  1:3379/140‑1 (MQ=255)                               gATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCgg                                                                                             <  1:964069/140‑1 (MQ=255)                                                  aGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGtt                                                                          <  2:606655/140‑1 (MQ=255)                                                  aGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGtt                                                                          <  1:793344/140‑1 (MQ=255)                                                      gTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACg                                                                      <  2:601400/140‑1 (MQ=255)                                                      gTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACg                                                                      <  2:955186/140‑1 (MQ=255)                                                        gggCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGt                                                                    <  2:899294/140‑1 (MQ=255)                                                                                        aaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑Accc                                   <  2:477434/140‑1 (MQ=17)                                                                                             aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑ACCCCCGGt                              <  2:810265/140‑1 (MQ=18)                                                                                                         tCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑ACCCCCGGTCGGGGCTTCTCa                  <  2:320715/140‑1 (MQ=17)                                                                                                         tCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑ACCCCCGGTCGGGGCTTCTCa                  <  2:835179/140‑1 (MQ=17)                                                                                                         tCGTACGAGCTCTTCTTAAAATATGGCGGTGAGGGGGGGGATTGGCTCGCTTCGCTCGCACTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑ACCCCCGGTCGGGGCTTCTCa                  <  2:68265/140‑1 (MQ=11)                                                                                                              cGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑ACCCCCGGTCGGGGCTTCTCATcccc             <  1:996158/140‑1 (MQ=17)                                                                                                              cGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑ACCCCCGGTCGGGGCTTCTCATcccc             <  2:345055/140‑1 (MQ=17)                                                                                                              cGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑ACCCCCGGTCGGGGCTTCTCATcccc             <  1:1008223/140‑1 (MQ=17)                                                                                                                 gCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑ACCCCCGGTCGGGGCTTCTCATCCCCCCg          <  1:164603/140‑1 (MQ=17)                                                                                                                 gCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑ACCCCCGGTCGGGGCTTCTCATCCCCCCg          <  2:405891/140‑1 (MQ=17)                                                                                                                 agacagcttTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑ACCCCCGGTCGGGGCTTCTCATCCCCCCg          <  1:954390/134‑1 (MQ=255)                                                                                                                   tcttctTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑ACCCCCGGTCGGGGCTTCTCATCCCCCCGgt        <  1:516931/140‑1 (MQ=17)                                                                                                                     ttcttTAAATATGGCGGTGA‑GGGGGGGGGTGACTCGCTTCGCTCCCCCCGCGGGCAGGCCCCCCCCCGCGGTCCCCGGCTTTCCCACCGGCTGGCGCCCGTTGTTGAACCCCCGGCCGGGG‑GTCTCTTCCCCCCCgggtg     >  2:279368/1‑140 (MQ=0)                                                                                                                      tcttTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑ACCCCCGGTCGGGGCTTCTCATCCCCCCGgtgtg     <  1:718126/140‑1 (MQ=17)                                                                                                                        tttAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑ACCCCCGGTCGGGGCTTCTCATCCCCCCGGtgtgtg   <  2:291118/140‑1 (MQ=17)                                                                                                                         ttAAATATGGCGGTGAGGGGGGGGGTTGACTCGCTTCGCTCGCCCTGCGGGGAGCCCCCTCACTGCGTTCACGGTCTGTCCCACTGGCTGTCGCCCGGTGTCG‑ACCCCCCCGCGGGGGTTTTCATCCCCCCCGggtgtgg  >  2:952182/1‑139 (MQ=11)                                                                                                                                         |                                                                                                                             TGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCG‑ACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGC  >  NC_000913/1096895‑1097153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑