| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,162,592 | (G)5→6 | coding (346/378 nt) | ycfL → | DUF1425 domain‑containing protein YcfL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,162,587 | 1 | . | G | 94.1% | 112.7 / 0.6 | 34 | coding (341/378 nt) | ycfL | DUF1425 domain‑containing protein YcfL |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (12/20); total (13/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01 | |||||||||||
GGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCT‑GGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCA > NC_000913/1162449‑1162718 | ggCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCT‑g > 2:549005/1‑140 (MQ=255)ggCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCT‑g > 2:8988/1‑140 (MQ=255) cAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCT‑ggg < 2:411876/140‑1 (MQ=255) gAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCT‑ggg‑ggg < 2:428070/140‑2 (MQ=255) ccGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCT‑ggg‑gggcgca < 2:410203/140‑6 (MQ=255) gTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGcaca < 1:549005/140‑1 (MQ=255) gTTCATTATCGTTTTTACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGACCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCa < 2:988712/140‑1 (MQ=255) cATTATCGTTTTTACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGAc < 2:708956/140‑1 (MQ=255) tATCGTTTTTACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACt < 1:19274/138‑1 (MQ=255) tATCGTTTTTACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACt > 2:19274/1‑138 (MQ=255) tCGTTTTTACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTAt < 2:808941/140‑1 (MQ=255) tACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATAtt > 1:791950/1‑140 (MQ=255) tGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGt > 2:450515/1‑140 (MQ=255) ggTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTa < 1:8988/140‑1 (MQ=255) ggTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCT‑GGGAGGCGCACAAAGTCAGa > 2:1042584/1‑123 (MQ=255) ggTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCT‑GGGAGGCGCACAAAGTCAGa < 1:1042584/123‑1 (MQ=255) aCGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGt < 2:626606/140‑1 (MQ=255) agagGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATc > 2:63210/1‑140 (MQ=255) agagGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATc > 2:582209/1‑140 (MQ=255) agagGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATc > 1:248721/1‑140 (MQ=255) ggCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTtga < 1:363862/140‑1 (MQ=255) cTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTtgatg > 2:16554/1‑140 (MQ=255) tGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAgggg > 1:993878/1‑127 (MQ=255) tGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAgggg < 2:993878/127‑1 (MQ=255) tGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTtgatga < 2:248721/140‑1 (MQ=255) tGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCt < 1:63210/140‑1 (MQ=255) aaGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGc < 1:16554/140‑1 (MQ=255) ggCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCt > 1:730405/1‑140 (MQ=255) ggCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCt > 1:478715/1‑140 (MQ=255) cGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACcgc < 2:730405/140‑1 (MQ=255) gCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGttt < 1:31652/140‑1 (MQ=255) acaTTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTtct < 2:277884/140‑1 (MQ=255) ggcggTAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCgg < 1:582209/140‑1 (MQ=255) tAACGCTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGgtgtg < 2:791950/140‑1 (MQ=255) cTGTACGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATCTTTCTCGCCGGGTGTGTgggg < 2:739516/140‑1 (MQ=255) cGGCAGCGCCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACg > 2:994885/1‑140 (MQ=255) cgcCAATTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAAcc > 1:447723/1‑140 (MQ=255) aaTTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCa < 1:994885/140‑1 (MQ=255) aaTTTTCTGGGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCa < 1:3924/140‑1 (MQ=255) | GGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCT‑GGG‑GGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATCTTGATGACAAAAATGAGTCGCTACGCCTTGATTACCGCGCTGGCGATGTTTCTCGCCGGGTGTGTGGGGCAACGTGAACCTGCA > NC_000913/1162449‑1162718 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |