Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,020	A→G	intergenic (‑234/‑143)	yaaI ← / → dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,020	0	A	G	100.0%	103.1 / NA	29	intergenic (‑234/‑143)	yaaI/dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/16);  total (13/16)

TGAATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGAC  >  NC_000913/11895‑12156                                                                                                                              |                                                                                                                                         tgaATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGAcc                                                                                                                            <  2:950504/140‑1 (MQ=255)          tGGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTtagt                                                                                                                   >  2:581237/1‑140 (MQ=255)             cTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTagtagt                                                                                                                <  1:380661/140‑1 (MQ=255)                cGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCaa                                                                                                             >  2:746403/1‑140 (MQ=255)                   ttcattcTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCg                                                                                                          <  2:1020823/140‑1 (MQ=255)                   ttcattcTTTATATGAATAAAATGGCGGTCAATTTTACGTCTTGGCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCATCCg                                                                                                          <  2:179657/140‑1 (MQ=255)                      attcTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCag                                                                                                       >  2:973872/1‑140 (MQ=255)                            tataTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCagtgagtg                                                                                                 >  1:403108/1‑140 (MQ=255)                            tataTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCagtgagtg                                                                                                 >  1:780480/1‑140 (MQ=255)                                  aaTAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCta                                                                                           >  2:849250/1‑139 (MQ=255)                                  aaTAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTg                                                                                           >  2:847753/1‑140 (MQ=255)                                            cTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATg                                                                                 >  2:493848/1‑140 (MQ=255)                                            cTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATg                                                                                 >  1:311926/1‑140 (MQ=255)                                               tCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGaaa                                                                              <  1:847753/140‑1 (MQ=255)                                                           cTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGa                                                                  >  1:341900/1‑140 (MQ=255)                                                             tGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGaaa                                                                >  1:39029/1‑140 (MQ=255)                                                               tCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAAcc                                                              >  1:568137/1‑140 (MQ=255)                                                                            cgAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGccc                                                 <  1:298293/140‑1 (MQ=255)                                                                                       cgcAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGAc                                      >  1:755838/1‑140 (MQ=255)                                                                                       cgcAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGAc                                      <  1:746403/140‑1 (MQ=255)                                                                                        gcAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACt                                     <  1:973872/140‑1 (MQ=255)                                                                                          aaaaGCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTca                                   <  1:795030/140‑1 (MQ=255)                                                                                              gCACAAAAAATTTTTGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAAc                               <  2:755838/140‑1 (MQ=255)                                                                                                           ttGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCg                  <  1:581237/140‑1 (MQ=255)                                                                                                            tGCATCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGa                 <  1:248365/140‑1 (MQ=255)                                                                                                               aTCTCCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAata              <  2:311926/140‑1 (MQ=255)                                                                                                                 ctcCCCCTTGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTACAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAatata            <  1:849250/140‑1 (MQ=255)                                                                                                                        ttGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGga     <  2:636482/140‑1 (MQ=255)                                                                                                                        ttGATGGCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGga     <  1:667933/140‑1 (MQ=255)                                                                                                                           atggCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGga     <  1:920723/137‑1 (MQ=255)                                                                                                                           atggCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGga     >  2:920723/1‑137 (MQ=255)                                                                                                                           atggCGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGAc  >  2:560174/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                         TGAATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGAC  >  NC_000913/11895‑12156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑