Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	209,600	A→G	intergenic (+20/‑79)	accA → / → ldcC	acetyl‑CoA carboxyltransferase subunit alpha/lysine decarboxylase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	209,600	0	A	G	100.0%	150.8 / NA	42	intergenic (+20/‑79)	accA/ldcC	acetyl‑CoA carboxyltransferase subunit alpha/lysine decarboxylase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/31);  total (11/31)

GATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAG  >  NC_000913/209463‑209729                                                                                                                                          |                                                                                                                                  gATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAggg                                                                                                                                 <  1:497790/140‑1 (MQ=255) gATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAggg                                                                                                                                 >  2:920714/1‑140 (MQ=255)   tGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGt                                                                                                                               <  2:750478/140‑1 (MQ=255)         cATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTc                                                                                                                         <  1:521815/140‑1 (MQ=255)           tCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCgg                                                                                                                       >  2:157081/1‑140 (MQ=255)                       cAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCtttttt                                                                                                           >  2:428030/1‑140 (MQ=255)                        aaCTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTa                                                                                                          <  2:353983/140‑1 (MQ=255)                             ctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGcc                                                                                                     <  1:157081/140‑1 (MQ=255)                             ctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGcc                                                                                                     <  1:203900/140‑1 (MQ=255)                                gCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACg                                                                                                  >  2:764202/1‑140 (MQ=255)                                  ggATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGt                                                                                                <  2:93974/140‑1 (MQ=255)                                   gATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGtt                                                                                               <  1:16673/140‑1 (MQ=255)                                     tCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTg                                                                                             >  2:615635/1‑140 (MQ=255)                                      cTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGa                                                                                            <  2:567547/140‑1 (MQ=255)                                      cTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGa                                                                                            <  1:106216/140‑1 (MQ=255)                                       tGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAg                                                                                           <  1:504808/140‑1 (MQ=255)                                       tGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAg                                                                                           <  1:764202/140‑1 (MQ=255)                                       tGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAg                                                                                           <  1:591755/140‑1 (MQ=255)                                       tGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAg                                                                                           <  1:428030/140‑1 (MQ=255)                                       tGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAg                                                                                           <  1:322556/140‑1 (MQ=255)                                       tGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAg                                                                                           <  2:966696/140‑1 (MQ=255)                                       tGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAg                                                                                           <  1:312043/140‑1 (MQ=255)                                          ccGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAg                                                                                        >  1:12143/1‑140 (MQ=255)                                              tctcGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTa                                                                                    >  2:651006/1‑140 (MQ=255)                                                   aCGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGAtt                                                                               >  2:408297/1‑140 (MQ=255)                                                          aaGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTaaggaag                                                                        >  2:222038/1‑140 (MQ=255)                                                                 gAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGATCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTc                                                                 >  1:353468/1‑140 (MQ=255)                                                                     aTTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCagg                                                             <  1:32104/140‑1 (MQ=255)                                                                     aTTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCagg                                                             <  1:920714/140‑1 (MQ=255)                                                                       ttAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCaggag                                                           <  1:841723/140‑1 (MQ=255)                                                                        tAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCaggagg                                                          <  1:408297/140‑1 (MQ=255)                                                                           aaaaTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAac                                                       <  2:815903/140‑1 (MQ=255)                                                                                         cAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCAtt                                         <  1:182209/140‑1 (MQ=255)                                                                                           gcgcCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGc                                       <  1:975500/140‑1 (MQ=255)                                                                                           gcgcCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGc                                       <  2:433275/140‑1 (MQ=255)                                                                                           gcgcCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGc                                       <  1:243047/140‑1 (MQ=255)                                                                                            cgcCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACAt                                                   <  1:791995/127‑1 (MQ=255)                                                                                            cgcCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACAt                                                   >  2:791995/1‑127 (MQ=255)                                                                                                   tgaGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATgg                               <  1:615635/140‑1 (MQ=255)                                                                                                              tACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGTGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGc                    <  1:568958/140‑1 (MQ=255)                                                                                                              tACGCGTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGc                    <  2:904833/140‑1 (MQ=255)                                                                                                                  cgTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCt                >  2:945756/1‑140 (MQ=255)                                                                                                                   gTAATTCGCAAAAGTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCtt               <  2:12143/140‑1 (MQ=255)                                                                                                                                gTTCTGAAAAGGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAg  <  2:20141/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  GATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAG  >  NC_000913/209463‑209729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑