Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,867,918 T→C N279S (AAC→AGC)  nlpD ← murein hydrolase activator NlpD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,867,9180TC100.0% 193.9 / NA 54N279S (AAC→AGC) nlpDmurein hydrolase activator NlpD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/23);  total (31/23)

GTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACA  >  NC_000913/2867788‑2868049
                                                                                                                                  |                                                                                                                                   
gTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGGGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCt                                                                                                                            >  2:648340/1‑140 (MQ=255)
         atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCC‑GTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGcc                                                                                                                  >  1:673590/1‑140 (MQ=255)
          ttATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGcc                                                                                                                  >  2:494069/1‑140 (MQ=255)
               tttGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAgg                                                                                                             >  1:787999/1‑140 (MQ=255)
                  gatgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGttt                                                                                                          >  1:1037560/1‑140 (MQ=255)
                     gatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGa                                                                                                       <  1:15203/140‑1 (MQ=255)
                       tAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATc                                                                                                     >  2:753843/1‑140 (MQ=255)
                       tAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATc                                                                                                     <  1:753843/140‑1 (MQ=255)
                              aTTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGc                                                                                              >  1:878119/1‑140 (MQ=255)
                              aTTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCC‑GTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGcc                                                                                             <  2:673590/140‑1 (MQ=255)
                               ttACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGcc                                                                                             <  2:878119/140‑1 (MQ=255)
                                 aCCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCt                                                                                           <  1:648340/140‑1 (MQ=255)
                                   cGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCa                                                                                         >  1:1004009/1‑140 (MQ=255)
                                   cGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCa                                                                                         >  2:1020170/1‑140 (MQ=255)
                                     tAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTAGATCACTTTGCCCTCAGt                                                                                       >  1:475462/1‑140 (MQ=255)
                                        ccGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCgg                                                                                    >  1:991859/1‑140 (MQ=255)
                                        ccGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCgg                                                                                    >  2:405088/1‑140 (MQ=255)
                                        ccGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCgg                                                                                    >  2:789928/1‑140 (MQ=255)
                                                cgcgTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGcc                                                                            >  2:317914/1‑140 (MQ=255)
                                                cgcgTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGcc                                                                            >  2:719986/1‑140 (MQ=255)
                                                  cgTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTTCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCCCAGTCGGCCAGCGCCAg                                                                          >  2:930015/1‑140 (MQ=255)
                                                  cgTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAg                                                                          >  1:75328/1‑140 (MQ=255)
                                                  cgTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAg                                                                          <  1:317914/140‑1 (MQ=255)
                                                        cAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGgag                                                                    <  1:1020170/140‑1 (MQ=255)
                                                         aGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTAGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGgaga                                                                   <  2:475462/140‑1 (MQ=255)
                                                          gCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGGCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGAt                                                                  <  1:467633/140‑1 (MQ=255)
                                                            aTAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTGCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAg                                                                >  2:973023/1‑140 (MQ=255)
                                                                     gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGgaga                                                                   <  1:884739/128‑1 (MQ=255)
                                                                     gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGgaga                                                                   >  2:884739/1‑128 (MQ=255)
                                                                     gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTgg                                                       >  1:447139/1‑140 (MQ=255)
                                                                     gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTgg                                                       >  1:456027/1‑140 (MQ=255)
                                                                     gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTgg                                                       >  1:371264/1‑140 (MQ=255)
                                                                     gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTgg                                                       >  1:785189/1‑140 (MQ=255)
                                                                     gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTgg                                                       >  1:775195/1‑140 (MQ=255)
                                                                      cgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGt                                                      <  2:75328/140‑1 (MQ=255)
                                                                      cgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGt                                                      <  2:991859/140‑1 (MQ=255)
                                                                          cATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGAt                                                  <  2:210380/140‑1 (MQ=255)
                                                                                      cgATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACa                                      >  1:1018703/1‑140 (MQ=255)
                                                                                        aTAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGt                                    <  1:973023/140‑1 (MQ=255)
                                                                                             tGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCt                               >  2:407096/1‑140 (MQ=255)
                                                                                             tGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGACGTACTGCTGACAGTCGGCt                               <  2:785189/140‑1 (MQ=255)
                                                                                                 tGTCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGt                           <  2:371264/140‑1 (MQ=255)
                                                                                                   tCCTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTtg                         >  1:402086/1‑140 (MQ=255)
                                                                                                     cTTTGCTGCCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTtgtg                       >  1:606512/1‑140 (MQ=255)
                                                                                                        tgctgcCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGtgctt                    <  2:402086/140‑1 (MQ=255)
                                                                                                        tgctgcCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGtgctt                    <  2:1037560/140‑1 (MQ=255)
                                                                                                         gctgcCTGCGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGtgcttg                   <  1:405088/140‑1 (MQ=255)
                                                                                                           tgcctgcGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGtgcttgct                 <  2:136412/140‑1 (MQ=255)
                                                                                                             cctgcGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGt               <  1:576312/140‑1 (MQ=255)
                                                                                                               tgcGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTg             >  1:577593/1‑140 (MQ=255)
                                                                                                               tgcGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTg             >  1:453434/1‑140 (MQ=255)
                                                                                                                gcGATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTgg            >  1:867435/1‑140 (MQ=255)
                                                                                                                  gATATCAATCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTa          <  2:867435/140‑1 (MQ=255)
                                                                                                                          tCCCCTTGCTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACa  <  2:1018703/140‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                   
GTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACA  >  NC_000913/2867788‑2868049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑