Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,935,327	T→C	Y365H (TAC→CAC)	fucP →	L‑fucose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,935,327	0	T	C	100.0%	171.3 / NA	49	Y365H (TAC→CAC)	fucP	L‑fucose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/26);  total (23/26)

CTTCGCACCACACAAAGTCCTGGCCGCCTACGCATTAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGTACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACTCCGGTCATGGGTTTTGTCAGTGACGCGGCGGGCA  >  NC_000913/2935197‑2935462                                                                                                                                   |                                                                                                                                        cTTCGCACCACACAAAGTCCTGGCCGCCTACGCATTAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCcaacaa                                                                                                                                >  1:786084/1‑140 (MQ=255)    cGCACCACACAAAGTCCTGGCCGCCTACGCATTAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAAtct                                                                                                                             <  2:593731/140‑1 (MQ=255)          acacaAAGTCCTGGCCGCCTACGCATTAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGc                                                                                                                       <  1:506087/140‑1 (MQ=255)          acacaAAGTCCTGGCCGCCTACGCATTAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGc                                                                                                                       >  2:617298/1‑140 (MQ=255)           cacaAAGTCCTGGCCGCCTACGCATTAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCt                                                                                                                      <  2:786084/140‑1 (MQ=255)           cacaAAGTCCTGGCCGCCTACGCATTAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCt                                                                                                                      <  2:1030970/140‑1 (MQ=255)                aGTCCTGGCCGCCTACGCATTAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCa                                                                                                                 >  2:537173/1‑140 (MQ=255)                      ggCCGCCTACGCATTAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGACATGTGGGCGTCATAGCGATGACTTTATGCAGCGCCTTTATGTCGATTCAGAACCCAACAATCTTCTCGCTGGGCATTAAGa                                                                                                           >  2:912998/1‑140 (MQ=255)                       gccgccTACGCATTAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGaa                                                                                                          <  1:658102/140‑1 (MQ=255)                            cTACGCATTAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCg                                                                                                     >  1:696634/1‑140 (MQ=255)                              aCGCATTAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGc                                                                                                   <  1:326580/140‑1 (MQ=255)                                  aTTAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAgg                                                                                               <  1:403002/140‑1 (MQ=255)                                    tAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGac                                                                                             <  2:270064/140‑1 (MQ=255)                                    tAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGac                                                                                             <  1:537173/140‑1 (MQ=255)                                           tATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAat                                                                                      >  2:718701/1‑140 (MQ=255)                                           tATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAat                                                                                      >  2:869940/1‑140 (MQ=255)                                              ggCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATg                                                                                   >  1:149323/1‑140 (MQ=255)                                                   tgtgCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCACTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAAGCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCg                                                                              <  2:471781/140‑1 (MQ=255)                                                    gtgCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGt                                                                             >  2:911955/1‑140 (MQ=255)                                                     tgCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTc                                                                            >  1:192292/1‑140 (MQ=255)                                                     tgCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTc                                                                            >  1:229252/1‑140 (MQ=255)                                                     tgCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTc                                                                            >  1:656485/1‑140 (MQ=255)                                                          gATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCa                                                                       <  2:671348/140‑1 (MQ=255)                                                          gATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCa                                                                       <  2:656485/140‑1 (MQ=255)                                                               cAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGtt                                                                  >  1:929718/1‑140 (MQ=255)                                                                     tCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCATCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGAcc                                                            <  1:911955/140‑1 (MQ=255)                                                                      cGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCt                                                                          <  1:326669/125‑1 (MQ=255)                                                                      cGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCt                                                                          >  2:326669/1‑125 (MQ=255)                                                                      cGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCa                                                           >  2:574702/1‑140 (MQ=255)                                                                          ggCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCattat                                                       <  2:696634/140‑1 (MQ=255)                                                                              gTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTggc                                                   <  1:617298/140‑1 (MQ=255)                                                                                taTGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTggcgg                                                 <  1:1008143/139‑1 (MQ=255)                                                                                 aTGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTggcggc                                                <  1:395859/140‑1 (MQ=255)                                                                                       gCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTAtt                                          >  1:534209/1‑140 (MQ=255)                                                                                             tAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACt                                    <  2:929718/140‑1 (MQ=255)                                                                                              aGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACTc                                   <  1:1007799/140‑1 (MQ=255)                                                                                                cccTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGcggcgg                                              >  1:889977/1‑127 (MQ=255)                                                                                                cccTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGcggcgg                                              <  2:889977/127‑1 (MQ=255)                                                                                                cccTGACTTTATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACTCCg                                 >  2:686662/1‑140 (MQ=255)                                                                                                       tttATGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACTCCGGTCATgg                          <  2:149323/140‑1 (MQ=255)                                                                                                          aTGCAGCGCCTTTATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACTCCGGTCATGGGtt                       >  2:203246/1‑140 (MQ=255)                                                                                                                     ttATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACTCCGGTCATGGGTTTTGTCAGTGAc            >  1:544024/1‑140 (MQ=255)                                                                                                                      tATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACTCCGGTCATGGGTTTTGTCAGTGAcg           <  2:229252/140‑1 (MQ=255)                                                                                                                      tATGTCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACTCCGGTCATGGGTTTTGTCAGTGAcg           <  2:192292/140‑1 (MQ=255)                                                                                                                          tCGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACTCCGGTCATGGGTTTTGTCAGTGACgcggc       <  1:628359/140‑1 (MQ=255)                                                                                                                           cGATTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACTCCGGTCATGGGTTTTGTCAGTGACgcggcg      <  1:869940/140‑1 (MQ=255)                                                                                                                             aTTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACTCCGGTCATGGGTTTTGTCAGTGACGCGGCggg    >  2:333492/1‑140 (MQ=255)                                                                                                                             aTTCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACTCCGGTCATGGGTTTTGTCAGTGACGCGGCggg    >  2:267314/1‑140 (MQ=255)                                                                                                                              ttCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACTCCGGTCATGGGTTTTGTCAGTGACGCGGCGGGc   >  2:258344/1‑140 (MQ=255)                                                                                                                               tCAGCACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACTCCGGTCATGGGTTTTGTCAGTGACGCGGCGGGCa  >  2:381825/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        CTTCGCACCACACAAAGTCCTGGCCGCCTACGCATTAATCGCTATGGCACTGTGCCTGATCTCAGCCTTCGCTGGCGGTCATGTGGGCTTAATAGCCCTGACTTTATGCAGCGCCTTTATGTCGATTCAGTACCCAACAATCTTCTCGCTGGGCATTAAGAATCTCGGCCAGGACACCAAATATGGTTCGTCCTTCATCGTTATGACCATTATTGGCGGCGGTATTGTCACTCCGGTCATGGGTTTTGTCAGTGACGCGGCGGGCA  >  NC_000913/2935197‑2935462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑