Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,971,669	G→A	E25K (GAA→AAA)	tas →	putative NADP(H)‑dependent aldo‑keto reductase Tas

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,971,669	0	G	A	100.0%	255.9 / NA	73	E25K (GAA→AAA)	tas	putative NADP(H)‑dependent aldo‑keto reductase Tas
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/34);  total (39/34)

TCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTACCCAGTACCTCCGCGCCCCGAAACGCAAGGGTTAACCGAAAC  >  NC_000913/2971548‑2971796                                                                                                                          |                                                                                                                                tctGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCg                                                                                                               >  1:905561/1‑140 (MQ=255)  ctGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGa                                                                                                              >  2:118826/1‑140 (MQ=255)   tGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGAc                                                                                                             >  2:941931/1‑140 (MQ=255)         ttttATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGcc                                                                                                          >  2:496469/1‑137 (MQ=255)         ttttATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGcc                                                                                                          <  1:496469/137‑1 (MQ=255)          tttATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGa                                                                                                              >  1:366642/1‑132 (MQ=255)          tttATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGa                                                                                                              <  2:366642/132‑1 (MQ=255)          tttATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACg                                                                                                      >  1:640349/1‑140 (MQ=255)            tataTTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGca                                                                                                    <  2:504492/140‑1 (MQ=255)                   cTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCg                                                                                             >  2:440086/1‑140 (MQ=255)                    ttATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGa                                                                                            >  1:215461/1‑140 (MQ=255)                     tATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGAc                                                                                           >  2:829736/1‑140 (MQ=255)                     tATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGAc                                                                                           >  1:356086/1‑140 (MQ=255)                       tGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTa                                                                                         >  1:960676/1‑140 (MQ=255)                       tGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTa                                                                                         >  1:352442/1‑140 (MQ=255)                            tGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTa                                                                                         <  2:44588/135‑1 (MQ=255)                            tGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTa                                                                                         >  1:44588/1‑135 (MQ=255)                            tGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCg                                                                                    >  1:601173/1‑140 (MQ=255)                             gATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGt                                                                                   >  1:143338/1‑140 (MQ=255)                             gATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGt                                                                                   >  2:838238/1‑140 (MQ=255)                             gATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGt                                                                                   >  2:869987/1‑140 (MQ=255)                              aTTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAACCAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTc                                                                                  >  2:440373/1‑140 (MQ=255)                                 ataAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCt                                                                               >  1:127347/1‑140 (MQ=255)                                 ataAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCt                                                                               >  2:1011567/1‑140 (MQ=255)                                  taAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTc                                                                              <  2:352442/140‑1 (MQ=255)                                  taAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTc                                                                              <  2:215461/140‑1 (MQ=255)                                  taAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTc                                                                              <  2:960676/140‑1 (MQ=255)                                   aaaaaGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCa                                                                             <  2:143338/140‑1 (MQ=255)                                        ggAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCa                                                                        >  2:659965/1‑140 (MQ=255)                                          aaaCGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCAtt                                                                      <  1:659965/140‑1 (MQ=255)                                          aaaCGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCAtt                                                                      <  2:601173/140‑1 (MQ=255)                                             cGGCTATGCAATATCACCGTATACCCCACACTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAAc                                                                   <  2:640349/140‑1 (MQ=255)                                                cTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCtt                                                                >  1:940957/1‑140 (MQ=255)                                                       atatCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACg                                                         <  2:187824/140‑1 (MQ=255)                                                       atatCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACg                                                         <  2:362053/140‑1 (MQ=255)                                                        tatCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGt                                                        >  2:806200/1‑140 (MQ=255)                                                          tCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCAt                                                                       <  2:777056/123‑1 (MQ=255)                                                          tCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCAt                                                                       >  1:777056/1‑123 (MQ=255)                                                          tCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACg                                                         >  2:546070/1‑137 (MQ=255)                                                          tCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACg                                                         <  1:546070/137‑1 (MQ=255)                                                          tCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTg                                                      >  1:934647/1‑140 (MQ=255)                                                           cACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGc                                                     >  2:93023/1‑140 (MQ=255)                                                            aCCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTACCGACGTTGcc                                                    <  2:940957/140‑1 (MQ=255)                                                               gTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGaa                                                 <  1:118826/140‑1 (MQ=255)                                                                tataCCCCACAGTTCCCTGGAAGTCAGCACGCTGGTGCTTGGCACGCTGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGaaa                                                >  2:82509/1‑140 (MQ=255)                                                                  taCCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATg                                              <  1:869987/140‑1 (MQ=255)                                                                  taCCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATg                                              >  1:886442/1‑140 (MQ=255)                                                                  taCCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATg                                              <  1:941931/140‑1 (MQ=255)                                                                    ccccACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTa                                            <  2:356086/140‑1 (MQ=255)                                                                    ccccACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTa                                            <  1:121179/140‑1 (MQ=255)                                                                    ccccACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTa                                            <  1:93023/140‑1 (MQ=255)                                                                     cccACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGCTGCCGAAATGTAc                                           >  2:768803/1‑140 (MQ=255)                                                                      ccACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTAcc                                          <  1:440086/140‑1 (MQ=255)                                                                       cacaGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTAccc                                         >  1:8127/1‑140 (MQ=255)                                                                          aGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTa                                            <  1:128759/134‑1 (MQ=255)                                                                          aGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTa                                            >  2:128759/1‑134 (MQ=255)                                                                            ttCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATg                                              <  2:307062/130‑1 (MQ=255)                                                                            ttCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATg                                              >  1:307062/1‑130 (MQ=255)                                                                              cGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATg                                              <  2:699542/128‑1 (MQ=255)                                                                              cGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATg                                              <  1:249262/128‑1 (MQ=255)                                                                              cGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATg                                              >  1:699542/1‑128 (MQ=255)                                                                              cGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATg                                              >  2:249262/1‑128 (MQ=255)                                                                                 tGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAAt                                               <  1:438786/124‑1 (MQ=255)                                                                                 tGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAAt                                               >  2:438786/1‑124 (MQ=255)                                                                                   gAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTACCCAGTACCTCcgcg                             <  2:127347/140‑1 (MQ=255)                                                                                        cAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTACCCAGTACCTCCGCGCCCCg                        <  2:934647/140‑1 (MQ=255)                                                                                        cAGCACGCTGGGGCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTACCCAGTACCTCCGCGCCCCg                        <  1:575699/140‑1 (MQ=255)                                                                                                    gCTTGGCACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTACCCAGTACCTCCGCGCCCCGAAACGCAAGGGt            <  1:70344/140‑1 (MQ=255)                                                                                                          cACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTACCCAGTACCTCCGCGCCCCGAAACGCAAGGGTTAAc        <  1:322319/138‑1 (MQ=255)                                                                                                          cACGATGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTACCCAGTACCTCCGCGCCCCGAAACGCAAGGGTTAAc        >  2:322319/1‑138 (MQ=255)                                                                                                              aTGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTACCCAGTACCTCCGCGCCCCGAAACGCAAGGGt            <  1:762745/130‑1 (MQ=255)                                                                                                              aTGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTACCCAGTACCTCCGCGCCCCGAAACGCAAGGGt            >  2:762745/1‑130 (MQ=255)                                                                                                              aTGACGTTTGGTAAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTACCCAGTACCTCCGCGCCCCGAAACGCAAGGGTTAACCGAAAc  >  1:240214/1‑140 (MQ=255)                                                                                                                          |                                                                                                                                TCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCAGGGCATTAACCTTATCGACGTTGCCGAAATGTACCCAGTACCTCCGCGCCCCGAAACGCAAGGGTTAACCGAAAC  >  NC_000913/2971548‑2971796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑