Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,022,559	T→C	V415A (GTA→GCA)	xdhD →	fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,022,559	0	T	C	100.0%	190.1 / NA	53	V415A (GTA→GCA)	xdhD	fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/29);  total (24/29)

TTGCCGATGCCGATGTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGTACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCA  >  NC_000913/3022430‑3022695                                                                                                                                  |                                                                                                                                         ttGCCGATGCCGATGTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCAc                                                                                                                                >  2:379662/1‑140 (MQ=255)    ccgatgccgatgTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTa                                                                                                                             <  2:462186/140‑1 (MQ=255)    ccgatgccgatgTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTa                                                                                                                             <  1:379662/140‑1 (MQ=255)    ccgatgccgatgTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTa                                                                                                                             <  1:270293/140‑1 (MQ=255)           cgatgTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACgccgcc                                                                                                                      <  2:427640/140‑1 (MQ=255)                   tCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTcgcg                                                                                                              <  1:330095/140‑1 (MQ=255)                     aTTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTcgcgcg                                                                                                            <  1:548658/140‑1 (MQ=255)                          gCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCg                                                                                                       >  2:950496/1‑140 (MQ=255)                             aaCCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTgg                                                                                                    >  1:242030/1‑140 (MQ=255)                             aaCCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTgg                                                                                                    >  2:75887/1‑140 (MQ=255)                             aaCCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTgg                                                                                                    >  2:535085/1‑140 (MQ=255)                             aaCCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTgg                                                                                                    <  1:532602/140‑1 (MQ=255)                                cTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCa                                                                                                 <  1:295533/140‑1 (MQ=255)                                     aCTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGaaa                                                                                            <  2:662549/140‑1 (MQ=255)                                          aCGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCa                                                                                       >  2:963351/1‑140 (MQ=255)                                          aCGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCa                                                                                       <  1:1008949/140‑1 (MQ=255)                                          aCGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCa                                                                                       >  1:805743/1‑140 (MQ=255)                                           cGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTgg                                                                                                    <  1:224102/126‑1 (MQ=255)                                           cGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTgg                                                                                                    >  2:224102/1‑126 (MQ=255)                                           cGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCAt                                                                                      >  1:1017193/1‑140 (MQ=255)                                           cGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCAt                                                                                      >  2:451614/1‑140 (MQ=255)                                                 cgcAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGtt                                                                                <  1:497804/140‑1 (MQ=255)                                                 cgcAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGtt                                                                                <  1:535085/140‑1 (MQ=255)                                                     gcagTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATg                                                                            <  1:937045/140‑1 (MQ=255)                                                         tGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCAt                                                                        >  1:239204/1‑140 (MQ=255)                                                             cGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTaaa                                                                    <  1:950496/140‑1 (MQ=255)                                                              gACTGAACCACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAag                                                                   >  1:545957/1‑140 (MQ=255)                                                                cTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAagag                                                                 >  1:913568/1‑140 (MQ=255)                                                                   aaaCACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGa                                                              >  2:481766/1‑140 (MQ=255)                                                                          tatCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTggcg                                                       >  2:1017579/1‑140 (MQ=255)                                                                          tatCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTggcg                                                       >  1:279662/1‑140 (MQ=255)                                                                                cTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGtt                                                 >  2:881878/1‑140 (MQ=255)                                                                                 tttACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGttt                                                >  2:119615/1‑140 (MQ=255)                                                                                          aTGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCaa                                       >  2:663064/1‑140 (MQ=255)                                                                                             gACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACa                                    >  1:807391/1‑140 (MQ=255)                                                                                               cGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAgg                                  <  2:913568/140‑1 (MQ=255)                                                                                               cGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAgg                                  >  1:841884/1‑140 (MQ=255)                                                                                                  cGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACa                               <  2:279662/140‑1 (MQ=255)                                                                                                  cGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACa                               <  1:75887/140‑1 (MQ=255)                                                                                                   gATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACAt                              >  2:977338/1‑140 (MQ=255)                                                                                                   gATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACAt                              <  1:481766/140‑1 (MQ=255)                                                                                                      cGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCct                           <  2:203925/140‑1 (MQ=255)                                                                                                        tCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCctgc                         <  1:977338/140‑1 (MQ=255)                                                                                                             ttATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGgaa                    <  2:545957/140‑1 (MQ=255)                                                                                                                   ccGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAgtg              <  2:805743/139‑1 (MQ=255)                                                                                                                   aCGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAgtg              <  2:239204/140‑1 (MQ=255)                                                                                                                   aCGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAgtg              <  2:807391/140‑1 (MQ=255)                                                                                                                      ccTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTgc           >  2:353174/1‑140 (MQ=255)                                                                                                                      ccTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTgc           <  2:1017193/140‑1 (MQ=255)                                                                                                                       cTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTgcg          >  1:6946/1‑140 (MQ=255)                                                                                                                       cTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTgcg          <  1:451614/140‑1 (MQ=255)                                                                                                                       cTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTgcg          <  1:119615/140‑1 (MQ=255)                                                                                                                               aGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCa  <  2:6946/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                         TTGCCGATGCCGATGTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGTACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCA  >  NC_000913/3022430‑3022695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑