Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,026,663	A→G	T300A (ACC→GCC)	guaD →	guanine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,026,663	0	A	G	100.0%	96.6 / NA	27	T300A (ACC→GCC)	guaD	guanine deaminase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/10);  total (17/10)

CATGATGGTTATCTGGATGTTTACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTA  >  NC_000913/3026528‑3026796                                                                                                                                        |                                                                                                                                      cATGATGGTTATCTGGATGTTTACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTc                                                                                                                                   <  1:614925/140‑1 (MQ=255)                      tACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCgg                                                                                                              >  1:154269/1‑140 (MQ=255)                       aCCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGc                                                                                                             >  1:736909/1‑140 (MQ=255)                                        aCCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGaaaaa                                                                                            >  1:207144/1‑140 (MQ=255)                                             tAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCAt                                                                                       >  1:283780/1‑140 (MQ=255)                                             tAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCAt                                                                                       >  1:651617/1‑140 (MQ=255)                                             tAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCAt                                                                                       >  2:543267/1‑140 (MQ=255)                                                    tgtgTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCagaa                                                                                <  2:736909/140‑1 (MQ=255)                                                             gCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTaa                                                                       >  1:173825/1‑140 (MQ=255)                                                                  cTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTgg                                                                  >  1:719520/1‑140 (MQ=255)                                                                    gCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGc                                                                >  1:289638/1‑140 (MQ=255)                                                                             tcGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACg                                                       >  1:1023118/1‑140 (MQ=255)                                                                                    aaaaGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCg                                                <  2:30122/140‑1 (MQ=255)                                                                                       agagTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTg                                             >  2:132808/1‑140 (MQ=255)                                                                                             ggATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAAGGGATATCGGTGCCGGaa                                       >  2:928601/1‑140 (MQ=255)                                                                                             ggATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGaa                                       <  2:651617/140‑1 (MQ=255)                                                                                              gATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAAc                                      >  2:859707/1‑140 (MQ=255)                                                                                                   tctcAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACtt                                 >  1:275000/1‑140 (MQ=255)                                                                                                       aGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCaa                             <  1:928601/140‑1 (MQ=255)                                                                                                       aGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCaa                             <  2:207144/140‑1 (MQ=255)                                                                                                       aGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCaa                             >  1:502748/1‑140 (MQ=255)                                                                                                        gCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAAc                            >  1:800484/1‑140 (MQ=255)                                                                                                          gAAACTAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACAt                          <  2:195399/140‑1 (MQ=255)                                                                                                              ccAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACAtgctg                      >  2:843893/1‑140 (MQ=255)                                                                                                                             cTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGaa       <  2:275000/140‑1 (MQ=255)                                                                                                                             cTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGaa       <  1:843893/140‑1 (MQ=255)                                                                                                                                  tGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTa  <  1:859707/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      CATGATGGTTATCTGGATGTTTACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTA  >  NC_000913/3026528‑3026796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑