Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,301,492	T→C	L3P (CTG→CCG)	yhbU →	putative peptidase YhbU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,301,492	0	T	C	100.0%	118.9 / NA	33	L3P (CTG→CCG)	yhbU	putative peptidase YhbU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/21);  total (12/21)

AAATTGCTGCTACCACCAATACAACTTTAACTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCG  >  NC_000913/3301361‑3301596                                                                                                                                    |                                                                                                         aaaTTGCTGCTACCACCAATACAACTTTAACTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTctgcc                                                                                                  <  1:388713/140‑1 (MQ=255)  aaTTGCTGCTACCACCAATACAACTTTAACTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTctgccc                                                                                                 <  2:904659/140‑1 (MQ=255)   aTTGCTGCTACCACCAATACAACTTTAACTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTctgccct                                                                                                >  1:889536/1‑140 (MQ=255)     tgctgcTACCACCAATACAACTTTAACTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTctgccctgc                                                                                              >  1:31312/1‑140 (MQ=255)               accaATACAACTTTAACTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAAtctc                                                                                    >  1:387312/1‑140 (MQ=255)                 caATACAACTTTAACTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTccc                                                                                  <  2:889536/140‑1 (MQ=255)                 caATACAACTTTAACTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTccc                                                                                  <  2:377819/140‑1 (MQ=255)                    tACAACTTTAACTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGgc                                                                               >  1:250484/1‑140 (MQ=255)                         cTTTAACTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTa                                                                          >  2:337172/1‑140 (MQ=255)                            tAACTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAgg                                                                       <  2:250484/140‑1 (MQ=255)                            tAACTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAgg                                                                       >  2:324875/1‑140 (MQ=255)                               cTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAggcgg                                                                    <  2:16086/140‑1 (MQ=255)                               cTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAggcgg                                                                    <  2:888305/140‑1 (MQ=255)                                   cTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCAt                                                                >  1:758141/1‑140 (MQ=255)                                   cTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCAt                                                                >  1:448080/1‑140 (MQ=255)                                              aaTTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGgcg                                                     <  2:798522/140‑1 (MQ=255)                                                  gTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGa                                                 <  1:27736/140‑1 (MQ=255)                                                   tCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGAt                                                <  2:31312/140‑1 (MQ=255)                                                   tCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGAt                                                <  2:881660/140‑1 (MQ=255)                                                   tCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGAt                                                <  1:337172/140‑1 (MQ=255)                                                    cGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATg                                               <  1:239660/140‑1 (MQ=255)                                                    cGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATg                                               <  2:758141/140‑1 (MQ=255)                                                    cGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATg                                               <  2:448080/140‑1 (MQ=255)                                                              ttAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTatat                                     <  2:387312/140‑1 (MQ=255)                                                              ttAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTatat                                     <  2:673482/140‑1 (MQ=255)                                                                     aaaTCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTa                              >  1:429152/1‑140 (MQ=255)                                                                       aTCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTaaa                            >  2:465738/1‑140 (MQ=255)                                                                             gTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCg                                   <  1:996797/127‑1 (MQ=255)                                                                             gTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCg                                   >  2:996797/1‑127 (MQ=255)                                                                             gTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTa                              >  2:363642/1‑132 (MQ=255)                                                                             gTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTa                              <  1:363642/132‑1 (MQ=255)                                                                                  ttAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCa                 <  1:469937/140‑1 (MQ=255)                                                                                                 tttGATAGCGCAACCTTCAGGAAAAATTATGGAGCCGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCg  <  1:324875/140‑1 (MQ=255)                                                                                                                                    |                                                                                                         AAATTGCTGCTACCACCAATACAACTTTAACTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAGCGCAACCTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCG  >  NC_000913/3301361‑3301596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑