Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,357,547	C→T	G941G (GGC→GGT)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,357,547	0	C	T	100.0%	141.1 / NA	42	G941G (GGC→GGT)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/18);  total (24/18)

CGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGCACCACGATATCTACTCTATCGAGGACTTAGCGCAGCTCATTTT  >  NC_000913/3357411‑3357678                                                                                                                                         |                                                                                                                                    cGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAggtggt                                                                                                                                  <  1:573105/140‑1 (MQ=255)        cTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGc                                                                                                                           >  2:393540/1‑140 (MQ=255)        cTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGc                                                                                                                           >  1:952662/1‑140 (MQ=255)          aCGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCg                                                                                                                         <  1:393540/140‑1 (MQ=255)                 caacaaAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATa                                                                                                                  >  2:338682/1‑140 (MQ=255)                  aacaaAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATaa                                                                                                                 >  2:143520/1‑140 (MQ=255)                       aGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGTGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCa                                                                                                            <  1:192809/140‑1 (MQ=255)                           tCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGAt                                                                                                                   >  2:71119/1‑129 (MQ=255)                           tCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGAt                                                                                                                   <  1:71119/129‑1 (MQ=255)                           tCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGcc                                                                                                        <  1:143520/140‑1 (MQ=255)                                           tGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTg                                                                                        >  1:669252/1‑140 (MQ=255)                                                       gCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTg                                                                            >  1:459620/1‑140 (MQ=255)                                                        cTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGc                                                                           >  1:280273/1‑140 (MQ=255)                                                         tttGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGcc                                                                          >  1:194498/1‑140 (MQ=255)                                                              ggTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAg                                                                     <  1:338682/140‑1 (MQ=255)                                                                ttACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTg                                                                   <  2:194498/140‑1 (MQ=255)                                                                 tACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTTACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGa                                                                  <  2:928399/140‑1 (MQ=255)                                                                  aCTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCg                                                                        <  1:763630/133‑1 (MQ=255)                                                                  aCTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCg                                                                        >  2:763630/1‑133 (MQ=255)                                                                   cTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACg                                                                >  1:717009/1‑140 (MQ=255)                                                                   cTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACg                                                                <  2:717009/140‑1 (MQ=255)                                                                   cTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACg                                                                >  1:127212/1‑140 (MQ=255)                                                                       ggCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTg                                                                   <  1:788719/133‑1 (MQ=255)                                                                       ggCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTg                                                                   >  2:788719/1‑133 (MQ=255)                                                                        gCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGAt                                                           >  1:602828/1‑140 (MQ=255)                                                                         cGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGAtc                                                          >  2:476492/1‑140 (MQ=255)                                                                                 gTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCccgcc                                                  >  1:268719/1‑140 (MQ=255)                                                                                  tCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCccgccg                                                 <  2:602828/140‑1 (MQ=255)                                                                                      tGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCcgccgccgc                                             >  2:129247/1‑140 (MQ=255)                                                                                      tGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCcgccgccgc                                             >  2:730602/1‑140 (MQ=255)                                                                                       gCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGca                                            <  2:268719/140‑1 (MQ=255)                                                                                       gCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGca                                            <  2:669252/140‑1 (MQ=255)                                                                                         cGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGcacc                                          <  2:576866/140‑1 (MQ=255)                                                                                         cGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGcacc                                          <  2:120342/140‑1 (MQ=255)                                                                                           aCGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGcaccac                                        <  1:476492/140‑1 (MQ=255)                                                                                                   cAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGCACCACGATATCTa                                <  2:280273/140‑1 (MQ=255)                                                                                                           aGTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGCACCACGATATCTACTCTATCg                        >  1:889815/1‑140 (MQ=255)                                                                                                            gTCGCCCAGGGCGCGAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGCACCACGATATCTACTCTATCGa                       <  2:952662/140‑1 (MQ=255)                                                                                                                         cgAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGCACCACGATATCTACTCTATCGAGGACTTAGCGCAg          >  1:751276/1‑140 (MQ=255)                                                                                                                         cgAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGCACCACGATATCTACTCTATCGAGGACTTAGCGCAg          >  1:415083/1‑140 (MQ=255)                                                                                                                          gAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGCACCACGATATCTACTCTATCGAGGACTTAGCGCAGc         >  2:413200/1‑140 (MQ=255)                                                                                                                          gAAGCCAGGCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGCACCACGATATCTACTCTATCGAGGACTTAGCGCAGc         >  1:847220/1‑140 (MQ=255)                                                                                                                                 ggCGAAGGTGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGCACCACGATATCTACTCTATCGAGGACTTAGCGCAGCTCAtttt  >  2:1013889/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    CGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAGGTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAGATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTCACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCGCCGCACCACGATATCTACTCTATCGAGGACTTAGCGCAGCTCATTTT  >  NC_000913/3357411‑3357678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑