Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,463,808	T→C	S295S (AGT→AGC)	gspK →	Type II secretion system protein GspK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,463,808	0	T	C	100.0%	220.5 / NA	60	S295S (AGT→AGC)	gspK	Type II secretion system protein GspK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/30);  total (30/30)

GAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGTATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCAT  >  NC_000913/3463673‑3463944                                                                                                                                        |                                                                                                                                         gAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCa                                                                                                                                      <  1:844646/140‑1 (MQ=255)    ggAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGcg                                                                                                                                   <  1:142164/140‑1 (MQ=255)         gctggctAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGaa                                                                                                                              <  2:991987/140‑1 (MQ=255)         gctggctAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGaa                                                                                                                              <  2:912031/140‑1 (MQ=255)         gctggctAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTGCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGaa                                                                                                                              <  2:598027/140‑1 (MQ=255)            ggctAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACaa                                                                                                                           >  1:903284/1‑140 (MQ=255)            ggctAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACaa                                                                                                                           >  1:277025/1‑140 (MQ=255)               tAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATcc                                                                                                                        <  1:85165/140‑1 (MQ=255)                    aCGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAggg                                                                                                                   >  1:471913/1‑140 (MQ=255)                       gATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATg                                                                                                                >  1:441000/1‑140 (MQ=255)                       gATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATg                                                                                                                >  1:889617/1‑140 (MQ=255)                                   tACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCatat                                                                                                    >  1:791455/1‑140 (MQ=255)                                      tGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATAtttt                                                                                                 >  1:720897/1‑140 (MQ=255)                                        ggCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATAtttttt                                                                                               >  2:927140/1‑140 (MQ=255)                                         gcgcAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATAttttttt                                                                                              <  2:903284/140‑1 (MQ=255)                                         gcgcAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATAttttttt                                                                                              <  2:405689/140‑1 (MQ=255)                                             agcagGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAAc                                                                                          >  1:1006707/1‑140 (MQ=255)                                             agcagGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAAc                                                                                          >  1:382958/1‑140 (MQ=255)                                              gcagGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAAcc                                                                                         <  2:720897/140‑1 (MQ=255)                                                  gATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTaa                                                                                     >  1:307128/1‑140 (MQ=255)                                                    tttCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAAc                                                                                   >  1:84171/1‑140 (MQ=255)                                                         ccGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAAcagcag                                                                              <  2:1006707/140‑1 (MQ=255)                                                          cGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCaga                                                                             <  2:441000/140‑1 (MQ=255)                                                          cGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCaga                                                                             >  1:891040/1‑140 (MQ=255)                                                                       cTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTa                                                                >  2:500612/1‑140 (MQ=255)                                                                       cTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTa                                                                >  1:491827/1‑140 (MQ=255)                                                                             gCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCg                                                          <  2:84171/140‑1 (MQ=255)                                                                                  ggTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGc                                                     <  2:889617/140‑1 (MQ=255)                                                                                     gAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGc                                                  <  1:557096/140‑1 (MQ=255)                                                                                     gAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGAGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGc                                                  >  2:646440/1‑140 (MQ=255)                                                                                      aaaaGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCa                                                 >  1:482411/1‑140 (MQ=255)                                                                                        aaGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAAc                                               <  2:891040/140‑1 (MQ=255)                                                                                             aTCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTctac                                          <  1:58409/140‑1 (MQ=255)                                                                                             aTCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTctac                                          >  2:640439/1‑140 (MQ=255)                                                                                             aTCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTctac                                          >  1:407136/1‑140 (MQ=255)                                                                                              tctcTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTctact                                         <  2:482411/140‑1 (MQ=255)                                                                                                 ctTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAAt                                      <  1:50172/140‑1 (MQ=255)                                                                                                     cccTATAGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATa                                  <  2:382958/140‑1 (MQ=255)                                                                                                          taGCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGAt                             >  1:669293/1‑140 (MQ=255)                                                                                                            gCCGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCg                           >  1:781843/1‑140 (MQ=255)                                                                                                              cGCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTg                         <  2:307128/140‑1 (MQ=255)                                                                                                               gCTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGa                        <  1:260699/140‑1 (MQ=255)                                                                                                                cTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGAt                       >  1:301780/1‑140 (MQ=255)                                                                                                                cTATTTTACGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGAt                       >  1:616243/1‑140 (MQ=255)                                                                                                                       aCGCTGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGaa                <  2:949073/140‑1 (MQ=255)                                                                                                                           tGAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGt            >  2:889897/1‑140 (MQ=255)                                                                                                                            gAGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGtt           <  1:869711/140‑1 (MQ=255)                                                                                                                             aGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTg          >  1:88507/1‑140 (MQ=255)                                                                                                                             aGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTg          >  1:838005/1‑140 (MQ=255)                                                                                                                             aGTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTAATGCCTGAATCGTTg          <  1:500612/140‑1 (MQ=255)                                                                                                                             aGTACGG‑AAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGa         <  1:652190/140‑1 (MQ=255)                                                                                                                              gTACGGAAAGCATCAGCGACGAACAATCCCAGGGGTGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCAGAAGGATCGTGATGCCTGAATGGTTGa         >  2:923959/1‑140 (MQ=255)                                                                                                                              gTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGa         <  1:889897/140‑1 (MQ=255)                                                                                                                              gTACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGa         <  2:616243/140‑1 (MQ=255)                                                                                                                               tACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGGAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGAt        >  2:973638/1‑140 (MQ=255)                                                                                                                               tACGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCAGATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGAt        >  2:717433/1‑140 (MQ=255)                                                                                                                                aCGGAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATg       <  2:407136/140‑1 (MQ=255)                                                                                                                                   gAAAGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTc    <  2:781843/140‑1 (MQ=255)                                                                                                                                    aaaGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCa   >  1:977450/1‑140 (MQ=255)                                                                                                                                     aaGCATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCAt  <  2:669293/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCTATTTTACGCTGAGTACGGAAAGTATCAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCAT  >  NC_000913/3463673‑3463944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑