Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,549,305	T→C	T256A (ACC→GCC)	malQ ←	4‑alpha‑glucanotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,549,305	0	T	C	100.0%	172.2 / NA	47	T256A (ACC→GCC)	malQ	4‑alpha‑glucanotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/24);  total (23/24)

CATCAAAGGCTGCCTGCCAGAACAGGCTGTCGCCCTGCTCTGCAACAAACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATAT  >  NC_000913/3549173‑3549433                                                                                                                                     |                                                                                                                                 cATCAAAGGCTGCCTGCCAGAACAGGCTGTCGCCCTGCTCTGCAACAAACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTg                                                                                                                           >  2:406618/1‑140 (MQ=255) cATCAAAGGCTGCCTGCCAGAACAGGCTGTCGCCCTGCTCTGCAACAAACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTg                                                                                                                           >  2:590375/1‑140 (MQ=255) cATCAAAGGCTGCCTGCCAGAACAGGCTGTCGCCCTGCTCTGCAACAAACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTg                                                                                                                           <  1:396271/140‑1 (MQ=255)            gcctgccAGAACAGGCTGTCGCCCTGCTCTGCAACAAACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGAc                                                                                                                >  1:451060/1‑140 (MQ=255)            gcctgccAGAACAGGCTGTCGCCCTGCTCTGCAACAAACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGAc                                                                                                                <  2:451060/140‑1 (MQ=255)            gcctgccAGAACAGGCTGTCGCCCTGCTCTGCAACAAACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGAc                                                                                                                >  2:476583/1‑140 (MQ=255)                         ggCTGTCGCCCTGCTCTGCAACAAACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAACCCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATcg                                                                                                   <  1:194219/140‑1 (MQ=255)                          gCTGTCGCCCTGCTCTGCAACAAACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATcgc                                                                                                  >  2:967147/1‑140 (MQ=255)                          gCTGTCGCCCTGCTCTGCAACAAACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATcgc                                                                                                  <  1:819275/140‑1 (MQ=255)                            tGTCGCCCTGCTCTGCAACAAACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGGCGGCATcgcgc                                                                                                <  2:1013715/140‑1 (MQ=255)                                     gCTCTGCAACAAACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGc                                                                                       <  2:693659/140‑1 (MQ=255)                                       tctGCAACAAACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAg                                                                                     <  1:278543/140‑1 (MQ=255)                                        ctGCAACAAACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGc                                                                                    >  2:772431/1‑140 (MQ=255)                                              caaACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGt                                                                              >  1:486036/1‑140 (MQ=255)                                                 aCTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGc                                                                           <  1:791511/140‑1 (MQ=255)                                                     gCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTgg                                                                       >  2:3549/1‑140 (MQ=255)                                                          aCGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGc                                                                  <  1:398257/140‑1 (MQ=255)                                                                   tCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGccac                                                         <  2:360673/140‑1 (MQ=255)                                                                   tCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGccac                                                         <  1:877043/140‑1 (MQ=255)                                                                   tCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGccac                                                         >  1:360673/1‑140 (MQ=255)                                                                          atcatcaCGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCt                                                  <  1:967147/140‑1 (MQ=255)                                                                            catcaCGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGa                                                >  1:732134/1‑140 (MQ=255)                                                                            catcaCGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGa                                                >  2:768899/1‑140 (MQ=255)                                                                            catcaCGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGa                                                >  1:821600/1‑140 (MQ=255)                                                                             atcaCGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAg                                               <  1:476583/140‑1 (MQ=255)                                                                                 cGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCctc                                           >  1:628910/1‑140 (MQ=255)                                                                                 cGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCctc                                           <  1:93165/140‑1 (MQ=255)                                                                                 cGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCctc                                           >  2:1026319/1‑140 (MQ=255)                                                                                    tGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTc                                        >  2:179890/1‑140 (MQ=255)                                                                                      cgcgAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGc                                      <  2:628910/140‑1 (MQ=255)                                                                                      cgcgAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGc                                      >  1:397486/1‑140 (MQ=255)                                                                                      cgcgAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGc                                      <  2:821600/140‑1 (MQ=255)                                                                                              cTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATgg                              <  1:1026319/140‑1 (MQ=255)                                                                                                     cGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCtt                       >  2:134449/1‑140 (MQ=255)                                                                                                     cGCCATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCtt                       >  2:32634/1‑140 (MQ=255)                                                                                                        cATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCaa                    <  1:406618/140‑1 (MQ=255)                                                                                                        cATTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCaa                    <  1:590375/140‑1 (MQ=255)                                                                                                         aTTCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAAc                   <  1:179890/140‑1 (MQ=255)                                                                                                          ttCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACg                  <  1:134449/140‑1 (MQ=255)                                                                                                           tCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACgg                 >  1:536407/1‑140 (MQ=255)                                                                                                           tCGTAATGCTGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACgg                 >  2:902786/1‑140 (MQ=255)                                                                                                                    tGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGtt             <  1:1029843/135‑1 (MQ=255)                                                                                                                    tGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGtt             >  2:1029843/1‑135 (MQ=255)                                                                                                                    tGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGt        <  2:536407/140‑1 (MQ=255)                                                                                                                    tGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGt        >  1:555757/1‑140 (MQ=255)                                                                                                                    tGTCATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGt        <  1:768899/140‑1 (MQ=255)                                                                                                                       cATTTTTAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGa     >  1:306372/1‑140 (MQ=255)                                                                                                                          ttttAGGGCGGCAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGatat  <  2:555757/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                 CATCAAAGGCTGCCTGCCAGAACAGGCTGTCGCCCTGCTCTGCAACAAACTGGCGAAACGCGGCCATCTGCTCATCATCACGTTGCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGCTAAGATGGAAATCTTCAACGGCGTTAACGTCGATAT  >  NC_000913/3549173‑3549433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑