Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	273,785	A→G	K313K (AAA→AAG)	ykfC →	CP4‑6 prophage; PF00078 domain‑containing protein YkfC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	273,785	0	A	G	100.0%	197.0 / NA	55	K313K (AAA→AAG)	ykfC	CP4‑6 prophage; PF00078 domain‑containing protein YkfC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/33);  total (22/33)

AGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAG  >  NC_000913/273648‑273918                                                                                                                                          |                                                                                                                                      agagAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCt                                                                                                                                     <  1:788180/140‑1 (MQ=255)  gagaAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTc                                                                                                                                    <  1:1039286/140‑1 (MQ=255)             cAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACAt                                                                                                                         >  1:300714/1‑140 (MQ=255)                tGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGa                                                                                                                      <  1:717624/140‑1 (MQ=255)                tGGAAACCCGCGGTGGCGTACTGCCACTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGa                                                                                                                      <  1:383755/140‑1 (MQ=255)                 ggAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGAt                                                                                                                     <  2:319334/140‑1 (MQ=255)                          cgGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTaaa                                                                                                            >  1:756433/1‑140 (MQ=255)                           ggTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTaaaa                                                                                                           <  2:909709/140‑1 (MQ=255)                            gTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGCGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAAt                                                                                                          >  1:401677/1‑140 (MQ=255)                               gCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATccc                                                                                                       <  1:508899/140‑1 (MQ=255)                               gCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATccc                                                                                                       <  1:850671/140‑1 (MQ=255)                               gCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATccc                                                                                                       <  1:556656/140‑1 (MQ=255)                               gCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATccc                                                                                                       <  1:308677/140‑1 (MQ=255)                                cGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATcccc                                                                                                      <  1:949181/140‑1 (MQ=255)                                cGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATcccc                                                                                                      <  2:300714/140‑1 (MQ=255)                                 gTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATccccc                                                                                                     <  1:265993/140‑1 (MQ=255)                                    cTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATg                                                                                                  <  1:978479/140‑1 (MQ=255)                                     tGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGt                                                                                                 >  1:298920/1‑140 (MQ=255)                                     tGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGt                                                                                                 >  2:185058/1‑140 (MQ=255)                                       ccGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGTCATCAGGG‑GGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTaa                                                                                              >  1:905953/1‑140 (MQ=255)                                            aTGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATg                                                                                                  <  1:938451/132‑1 (MQ=255)                                            aTGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATg                                                                                                  >  2:938451/1‑132 (MQ=255)                                              gCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACgg                                                                                        <  1:91437/140‑1 (MQ=255)                                                   tgatTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTa                                                                                   <  1:195672/140‑1 (MQ=255)                                                    gatTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTAt                                                                                  <  1:185058/140‑1 (MQ=255)                                                       tttGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGa                                                                                           <  1:235873/128‑1 (MQ=255)                                                       tttGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGa                                                                                           >  2:235873/1‑128 (MQ=255)                                                                  cGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGcaca                                                                    >  1:357957/1‑140 (MQ=255)                                                                           cACCAAAGCACAGGTGGAAGTCATCAGGG‑GGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCg                                                          <  2:905953/140‑1 (MQ=255)                                                                           cACCAAAGCACAGGAGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCCGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTc                                                           >  2:245434/1‑140 (MQ=255)                                                                            aCCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCg                                                          >  1:956841/1‑140 (MQ=255)                                                                            aCCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCg                                                          >  1:322337/1‑140 (MQ=255)                                                                             ccAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGca                                                                      <  1:124815/127‑1 (MQ=255)                                                                             ccAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGca                                                                      >  2:124815/1‑127 (MQ=255)                                                                               aaaGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCaa                                                       <  2:322337/140‑1 (MQ=255)                                                                                  gCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACg                                                    <  2:128031/140‑1 (MQ=255)                                                                                        gTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCg                                              >  2:71060/1‑140 (MQ=255)                                                                                           gAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTa                                           >  1:966177/1‑140 (MQ=255)                                                                                           gAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTa                                           >  1:648725/1‑140 (MQ=255)                                                                                                cATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCg                                      >  1:848950/1‑140 (MQ=255)                                                                                                cATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAAGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCg                                      <  1:298393/140‑1 (MQ=255)                                                                                                   cAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCgaga                                   <  2:540627/140‑1 (MQ=255)                                                                                                       gaggagTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCg                               <  2:357957/140‑1 (MQ=255)                                                                                                        aggagTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGa                              <  2:966177/140‑1 (MQ=255)                                                                                                        aggagTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGa                              <  2:298920/140‑1 (MQ=255)                                                                                                         ggagTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAg                             <  2:401677/140‑1 (MQ=255)                                                                                                          gagTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGt                            >  2:154067/1‑140 (MQ=255)                                                                                                          gagTGTCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGt                            <  2:956841/140‑1 (MQ=255)                                                                                                              gtCGGGGTGTGCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGtc                        >  1:188277/1‑140 (MQ=255)                                                                                                                     tgtgCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGa                 >  1:890619/1‑140 (MQ=255)                                                                                                                     tgtgCTCGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGa                 <  2:756433/140‑1 (MQ=255)                                                                                                                           cGAAGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGc           <  1:882843/140‑1 (MQ=255)                                                                                                                              aGGCAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAgg        >  1:959123/1‑140 (MQ=255)                                                                                                                                 cAGTCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGgaga     <  1:154067/140‑1 (MQ=255)                                                                                                                                    tCTGAAGCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAg  >  1:488878/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      AGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAG  >  NC_000913/273648‑273918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑