Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,593,991	T→C	L72L (TTA→TTG)	livG ←	branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,593,991	0	T	C	100.0%	136.9 / NA	39	L72L (TTA→TTG)	livG	branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/21);  total (18/21)

CAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTT  >  NC_000913/3593856‑3594127                                                                                                                                        |                                                                                                                                         cAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAAc                                                                                                                                      <  1:270424/140‑1 (MQ=255) cAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAAc                                                                                                                                      <  2:766371/140‑1 (MQ=255)    gCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCtt                                                                                                                                   >  2:1043527/1‑140 (MQ=255)     ccAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTc                                                                                                                                  <  1:250995/140‑1 (MQ=255)      cAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTcc                                                                                                                                 >  2:366351/1‑140 (MQ=255)              aGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAAc                                                                                                                                      <  2:66694/127‑1 (MQ=255)              aGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAAc                                                                                                                                      >  1:66694/1‑127 (MQ=255)              aGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCt                                                                                                                          <  2:972246/139‑1 (MQ=255)              aGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCt                                                                                                                          >  1:972246/1‑139 (MQ=255)              aGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTg                                                                                                                         >  2:808210/1‑140 (MQ=255)              aGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTg                                                                                                                         >  1:338770/1‑140 (MQ=255)                cccGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGAt                                                                                                                       >  2:669603/1‑140 (MQ=255)                   ggTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATcgc                                                                                                                    >  1:180546/1‑140 (MQ=255)                    gTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATcgcg                                                                                                                   <  2:223562/140‑1 (MQ=255)                     ttttCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGa                                                                                                                        >  1:1021963/1‑134 (MQ=255)                     ttttCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGa                                                                                                                        <  2:1021963/134‑1 (MQ=255)                                        cgcCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCgg                                                                                               <  1:808210/140‑1 (MQ=255)                                        cgcCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCgg                                                                                               <  1:669603/140‑1 (MQ=255)                                        cgcCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCgg                                                                                               <  2:271245/140‑1 (MQ=255)                                        cgcCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCgg                                                                                               <  2:109884/140‑1 (MQ=255)                                         gcCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGt                                                                                              >  1:679479/1‑140 (MQ=255)                                                     ttttCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGaa                                                                                  <  1:245567/140‑1 (MQ=255)                                                      tttCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAAt                                                                                 <  2:338770/140‑1 (MQ=255)                                                           aTTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGt                                                                            <  1:54541/140‑1 (MQ=255)                                                             tACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCa                                                                          <  1:739722/140‑1 (MQ=255)                                                              aCCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCGCGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAg                                                                         >  2:974103/1‑140 (MQ=255)                                                                    aTTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGtt                                                                   >  2:560130/1‑140 (MQ=255)                                                                                         tGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGc                                              <  1:539445/140‑1 (MQ=255)                                                                                                aGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTa                                       >  2:984365/1‑140 (MQ=255)                                                                                                    gcgcACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGc                                   >  1:776432/1‑140 (MQ=255)                                                                                                      gcACCACGCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCg                                 <  2:180546/140‑1 (MQ=255)                                                                                                             gCCCATGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACg                          >  2:292334/1‑140 (MQ=255)                                                                                                                  tGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACg                     >  1:63479/1‑140 (MQ=255)                                                                                                                  tGCGGGCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACg                     >  2:560520/1‑140 (MQ=255)                                                                                                                      ggCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGGAGTAAAATGGTGCCCCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGAtct                 <  2:961721/140‑1 (MQ=255)                                                                                                                      ggCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGAtct                 <  2:776432/140‑1 (MQ=255)                                                                                                                       gCAATTTGCTGCCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGAtctc                >  1:81202/1‑140 (MQ=255)                                                                                                                              gctgcCCCGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCggg         <  2:63479/140‑1 (MQ=255)                                                                                                                                     cGGCAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGtt  <  1:984365/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTT  >  NC_000913/3593856‑3594127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑