Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,749,860	T→C	L210P (CTG→CCG)	sgbU →	putative L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,749,860	0	T	C	100.0%	208.7 / NA	58	L210P (CTG→CCG)	sgbU	putative L‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/29);  total (29/29)

AAATGGAAAAAATGGGACGAGATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATCGCGGTTCTTTCCTGATT  >  NC_000913/3749724‑3749993                                                                                                                                         |                                                                                                                                      aaaTGGAAAAAATGGGACGAGATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGaa                                                                                                                                    <  1:113383/140‑1 (MQ=255)  aaTGGAAAAAATGGGACGAGATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGaaa                                                                                                                                   >  2:844270/1‑140 (MQ=255)   aTGGAAAAAATGGGACGAGATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAg                                                                                                                                  >  1:377810/1‑140 (MQ=255)    tGGAAAAAATGGGACGAGATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGa                                                                                                                                 >  1:870938/1‑140 (MQ=255)    tGGAAAAAATGGGACGAGATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGa                                                                                                                                 <  1:431978/140‑1 (MQ=255)      gAAAAAATGGGACGAGATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGaca                                                                                                                               <  1:937240/140‑1 (MQ=255)                 aCGAGATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTc                                                                                                                    >  2:830596/1‑140 (MQ=255)                         cTCGCCTCACCGTGGTTCCCCGTCTCCCCGGACGTCGGCACCCTCCGCGCCTGGGGAAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCa                                                                                                            <  2:467096/140‑1 (MQ=255)                         cTCGCCTCACCGTGGTTCACCGTCTCCCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCa                                                                                                            <  1:43561/140‑1 (MQ=255)                         cTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCa                                                                                                            <  1:830596/140‑1 (MQ=255)                         cTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCa                                                                                                            <  1:570087/140‑1 (MQ=255)                          tCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCaa                                                                                                           >  2:1024156/1‑140 (MQ=255)                              cTCACCGTGGTTCACCGTCTCCCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTc                                                                                                       <  1:381675/140‑1 (MQ=255)                                 aCCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCg                                                                                                    >  2:339738/1‑140 (MQ=255)                                   cGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGa                                                                                                  <  1:339738/140‑1 (MQ=255)                                   cGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGa                                                                                                  <  2:870938/140‑1 (MQ=255)                                           aCCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCcg                                                                                          >  1:1025446/1‑140 (MQ=255)                                            ccGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCcgc                                                                                         >  2:318962/1‑140 (MQ=255)                                               tCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGAc                                                                                      >  1:676919/1‑140 (MQ=255)                                                  aCCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTg                                                                                   >  2:465711/1‑140 (MQ=255)                                                  aCCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTg                                                                                   >  1:986752/1‑140 (MQ=255)                                                  aCCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTg                                                                                   <  1:1024156/140‑1 (MQ=255)                                                  aCCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTg                                                                                   >  1:812727/1‑140 (MQ=255)                                                      ggccggcGGCACCCTCACCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGt                                                                               <  2:676919/134‑1 (MQ=255)                                                       gACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGtt                                                                              >  1:120307/1‑140 (MQ=255)                                                                             ggggCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTc                                                        >  2:141283/1‑140 (MQ=255)                                                                                   atgatgTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGc                                                  <  2:1025446/140‑1 (MQ=255)                                                                                   atgatgTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGc                                                  <  2:120307/140‑1 (MQ=255)                                                                                     gatgTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCAt                                                <  1:844270/140‑1 (MQ=255)                                                                                            ccgccgAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCaaa                                         <  1:287849/140‑1 (MQ=255)                                                                                            ccgccgAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCaaa                                         >  2:287849/1‑140 (MQ=255)                                                                                              gccgAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAAc                                       >  1:636932/1‑140 (MQ=255)                                                                                              gccgAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAAc                                       >  2:494310/1‑140 (MQ=255)                                                                                              gccgAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAAc                                       >  2:316362/1‑140 (MQ=255)                                                                                                   aCTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGc                                  >  1:210164/1‑140 (MQ=255)                                                                                                    cTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCa                                 <  1:838927/140‑1 (MQ=255)                                                                                                    cTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCa                                 <  1:494310/140‑1 (MQ=255)                                                                                                            gggCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGa                         >  1:979646/1‑140 (MQ=255)                                                                                                             ggCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAAc                                       >  2:920534/1‑125 (MQ=255)                                                                                                             ggCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAAc                                       <  1:920534/125‑1 (MQ=255)                                                                                                             ggCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTgga                        >  1:517914/1‑138 (MQ=255)                                                                                                             ggCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGaa                        >  2:280430/1‑140 (MQ=255)                                                                                                             ggCATTGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGaa                        >  2:927364/1‑140 (MQ=255)                                                                                                                  tGACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATc                   >  2:1008179/1‑140 (MQ=255)                                                                                                                   gACCGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGGACTATcg                  <  2:517914/140‑1 (MQ=255)                                                                                                                     ccGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATcgcg                <  2:986752/140‑1 (MQ=255)                                                                                                                     ccGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATcgcg                <  1:316362/140‑1 (MQ=255)                                                                                                                     ccGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATcgcg                <  2:812727/140‑1 (MQ=255)                                                                                                                     ccGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATcgcg                <  2:377810/140‑1 (MQ=255)                                                                                                                     ccGTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATcgcg                <  2:210164/140‑1 (MQ=255)                                                                                                                       gTATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATCGCGGt              >  1:417323/1‑140 (MQ=255)                                                                                                                        tATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATcgcg                <  1:126435/137‑1 (MQ=255)                                                                                                                        tATCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATcgcg                >  2:126435/1‑137 (MQ=255)                                                                                                                         aTCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATCGCGGTTc            >  1:412861/1‑140 (MQ=255)                                                                                                                         aTCGCCGCGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATCGCGGTTc            <  2:979646/140‑1 (MQ=255)                                                                                                                            gccgcGATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTTCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATCGCGGTTCttt         >  2:917783/1‑140 (MQ=255)                                                                                                                              cgcgATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATCGCGGTTCTTTcc       <  1:1008179/140‑1 (MQ=255)                                                                                                                               gcgATCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATCGCGGTTCTTTCCt      <  2:412861/140‑1 (MQ=255)                                                                                                                                   tCCACCCGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATCGCGGTTCTTTCCTGAtt  <  1:280430/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      AAATGGAAAAAATGGGACGAGATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCGATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATCGCGGTTCTTTCCTGATT  >  NC_000913/3749724‑3749993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑