Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,775,191	T→C	G256G (GGT→GGC)	mtlD →	mannitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,775,191	0	T	C	100.0%	185.1 / NA	52	G256G (GGT→GGC)	mtlD	mannitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/22);  total (30/22)

CACCCTGAACACGGGTCATGCTATAACCGCGTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCAC  >  NC_000913/3775065‑3775315                                                                                                                               |                                                                                                                             cACCCTGAACACGGGTCATGCTATAACCGCGTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCa                                                                                                                 >  1:868488/1‑140 (MQ=255)       gAACACGGGTCATGCTATAACCGCGTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCt                                                                                                           >  2:911512/1‑140 (MQ=255)         acacGGGTCATGCTATAACCGCGTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTAc                                                                                                         >  2:198893/1‑140 (MQ=255)              ggTCATGCTATAACCGCGTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCtt                                                                                                    <  1:446946/140‑1 (MQ=255)                  ctGCTTTATCCGCGTTTCTCGGAAACCTGGCCGGTCATCAGACCCTTCGTGACGCGATTCTCGCCGAGAAAATCCGCGCGGTGGTAACAGGTGCGCTGGAAGAAAGTGGCGCCGTATTGATCATGCGCTTCGGCTATgtc                                                                                                <  2:521023/139‑3 (MQ=255)                  aTGCTATAACCGCGTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAg                                                                                                               <  1:1041714/125‑1 (MQ=255)                  aTGCTATAACCGCGTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAg                                                                                                               >  2:1041714/1‑125 (MQ=255)                  aTGCTATAACCGCGTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGAc                                                                                                >  2:507818/1‑140 (MQ=255)                          aCCGCGTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACaa                                                                                        >  2:670931/1‑140 (MQ=255)                             gcgTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACg                                                                                               <  2:640594/130‑1 (MQ=255)                             gcgTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACg                                                                                               >  1:640594/1‑130 (MQ=255)                              cgTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCAt                                                                                    >  2:1031957/1‑140 (MQ=255)                              cgTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCAt                                                                                    >  1:349569/1‑140 (MQ=255)                              cgTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCAt                                                                                    >  2:200339/1‑140 (MQ=255)                                tACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATgc                                                                                  >  2:98730/1‑140 (MQ=255)                                tACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATgc                                                                                  >  1:448053/1‑140 (MQ=255)                                               gCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGaa                                                                   >  2:1034695/1‑140 (MQ=255)                                               gCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGaa                                                                   >  1:822230/1‑140 (MQ=255)                                                               ttCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGtttt                                                   >  2:235277/1‑140 (MQ=255)                                                               ttCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGtttt                                                   <  1:911512/140‑1 (MQ=255)                                                                tCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAGGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTg                                                  >  1:699730/1‑140 (MQ=255)                                                                tCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTg                                                  >  2:336443/1‑140 (MQ=255)                                                                tCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTg                                                  >  1:990967/1‑140 (MQ=255)                                                                      cgcgATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAAcc                                            <  1:1031957/140‑1 (MQ=255)                                                                      cgcgATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAAcc                                            <  1:1034695/140‑1 (MQ=255)                                                                      cgcgATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAAc                                             >  1:896910/1‑139 (MQ=255)                                                                      cgcgATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAAc                                             <  2:896910/139‑1 (MQ=255)                                                                         gATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTga                                                 >  1:1003304/1‑132 (MQ=255)                                                                         gATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTga                                                 <  2:1003304/132‑1 (MQ=255)                                                                              tcGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTg                                    <  1:198893/140‑1 (MQ=255)                                                                                gacgaGAAAATCCGCGCGGTGGTAAAGGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGaa                                  <  2:699730/140‑1 (MQ=255)                                                                                  cgaGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAg                                <  1:507818/140‑1 (MQ=255)                                                                                     gaAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTg                                    <  2:996093/133‑1 (MQ=255)                                                                                     gaAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTg                                    >  1:996093/1‑133 (MQ=255)                                                                                     gaAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAgatg                             >  2:455425/1‑140 (MQ=255)                                                                                           ccGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTag                       <  2:822230/140‑1 (MQ=255)                                                                                                     gtAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAgg             <  1:805967/140‑1 (MQ=255)                                                                                                     gtAAAAGGTGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAgg             <  1:670931/140‑1 (MQ=255)                                                                                                             tGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGc     >  1:175477/1‑140 (MQ=255)                                                                                                             tGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGc     >  1:878082/1‑140 (MQ=255)                                                                                                             tGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGc     >  1:874100/1‑140 (MQ=255)                                                                                                             tGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGc     >  2:593888/1‑140 (MQ=255)                                                                                                             tGCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGc     >  1:439612/1‑140 (MQ=255)                                                                                                             cgCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCACCGGCTTTCAGGCTGACAAGAATGCGGGGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGCAAGATGATGTAGAGCGCGTAGGCCGTCAGc     >  2:917114/2‑140 (MQ=255)                                                                                                              gCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGcc    <  1:200339/140‑1 (MQ=255)                                                                                                              gCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGcc    <  1:593888/140‑1 (MQ=255)                                                                                                              gCGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGcc    <  2:175477/140‑1 (MQ=255)                                                                                                               cGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTagag                     <  2:112162/122‑1 (MQ=255)                                                                                                               cGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTagag                     >  1:112162/1‑122 (MQ=255)                                                                                                               cGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCa   >  2:626354/1‑140 (MQ=255)                                                                                                               cGATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCa   <  1:520362/140‑1 (MQ=255)                                                                                                                gATGGAAGAAAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCAc  <  2:874100/140‑1 (MQ=255)                                                                                                                      agaaAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCAc  <  2:807617/134‑1 (MQ=255)                                                                                                                      agaaAGTGGCGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCAc  >  1:807617/1‑134 (MQ=255)                                                                                                                            tggcgCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCa   <  1:331758/127‑1 (MQ=255)                                                                                                                               |                                                                                                                             CACCCTGAACACGGGTCATGCTATAACCGCGTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCAC  >  NC_000913/3775065‑3775315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑