Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,781,355	G→A	A47A (GCG→GCA)	trmL →	tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,781,355	0	G	A	100.0%	75.2 / NA	23	A47A (GCG→GCA)	trmL	tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/6);  total (17/6)

TAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTAT  >  NC_000913/3781219‑3781487                                                                                                                                         |                                                                                                                                     tAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCaggg                                                                                                                                   <  2:155918/140‑1 (MQ=255)  aaaCATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGc                                                                                                                                  >  1:758023/1‑140 (MQ=255)      aTCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGa                                                                                                                              <  1:29425/140‑1 (MQ=255)      aTCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCAGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGa                                                                                                                              >  1:263068/1‑140 (MQ=255)          tACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTAt                                                                                                                          >  2:235064/1‑140 (MQ=255)             tttACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCAc                                                                                                                       <  2:126920/140‑1 (MQ=255)                      cAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCAc                                                                                                                       <  2:993044/131‑1 (MQ=255)                      cAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCAc                                                                                                                       >  1:993044/1‑131 (MQ=255)                                 ccAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGTTAcgcg                                                                                                   >  1:611289/1‑140 (MQ=255)                                                 tcatcCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGtcat                                                                                               >  1:284716/1‑128 (MQ=255)                                                 tcatcCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGtcat                                                                                               <  2:284716/128‑1 (MQ=255)                                                    tcCGTCTTTGCGATAATCCCGCCTTTCTTCTGCATATCATCAACCCGTGGGGATTTGCATGGGCCGATAGGGGCCTGCGCCGCGCAGGGTTGGATTACCACGATTTTACCGCCGATACGCGTCATCATGACTATcgcgcg                                                                                <  2:400301/140‑1 (MQ=255)                                                        tCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTcc                                                                            >  2:491381/1‑140 (MQ=255)                                                            tGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGa                                                                        >  1:607911/1‑140 (MQ=255)                                                              cgcTAATACCGGCTTTCGTCTGCATATCATCGAACCTATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGATACGCGTCATCATGACTATCGCGCGTTCCTCGAAg                                                                      >  1:400301/1‑140 (MQ=255)                                                              cgcTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAg                                                                      >  1:485147/1‑140 (MQ=255)                                                                         gCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATccc                                                           >  1:481821/1‑140 (MQ=255)                                                                              cGTCTGAATATCATCGAACCGAGGGGTTTTGCCGGGGACGATAAGCGCCGGCGCCGCGCGGGGTTGGACTATCCCGATTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAgcg                                                      <  2:481821/140‑1 (MQ=255)                                                                               gTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAgcgc                                                     >  2:16385/1‑140 (MQ=255)                                                                                       atcatcGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGc                                             >  1:507887/1‑140 (MQ=255)                                                                                        tcatcGAACCGATGGGTTTTGCCTGGGACGAAAAGCGCCTGCGCCGCGCAGGGTTGGACTATCACGATTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGcc                                            <  2:1013281/140‑1 (MQ=255)                                                                                              aaCCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCTCAGCGCCTGTTCGCCCTcacc                                      >  1:392954/1‑140 (MQ=255)                                                                                              aaCCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTcacc                                      >  2:792834/1‑140 (MQ=255)                                                                                                 gggtGGGATTTGCCGGGGACGATAAGCGCCTGCGCCGGGCGGGGCTGGACTATCACGATTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACg                                   <  2:239057/137‑1 (MQ=255)                                                                                                      ggttttGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAgg                              <  1:16385/137‑1 (MQ=255)                                                                                                      ggATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCACCAGCGCCTGTTCGCCCTCACCACGAAAgg                              <  2:611289/140‑1 (MQ=255)                                                                                                                 ggACGATAAGCGCCTGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTc                   >  2:530692/1‑140 (MQ=255)                                                                                                                               tGCGCCGCGCAGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGc     >  1:363466/1‑140 (MQ=255)                                                                                                                                  cccgcGAGGGGTTGGACAACCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTAt  <  2:758023/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTAT  >  NC_000913/3781219‑3781487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑