Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,865,335	T→C	E313E (GAA→GAG)	yidE ←	putative transport protein YidE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,865,335	0	T	C	100.0%	248.1 / NA	68	E313E (GAA→GAG)	yidE	putative transport protein YidE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/34);  total (34/34)

CATTGGCAACGGCATCAATTGCGGACGGACGCCCCACCAGATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGTTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATCACCAGTTGCGCAT  >  NC_000913/3865202‑3865468                                                                                                                                      |                                                                                                                                      caTTGGCAACGGCATCAATTGCGGACGGACGCCCCACCAGATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTa                                                                                                                                 <  2:969895/140‑1 (MQ=255)  aTTGGCAACGGCATCAATTGCGGACGGACGCCCCACCAGATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTaa                                                                                                                                <  2:995491/140‑1 (MQ=255)  aTTGGCAACGGCATCAATTGCGGACGGACGCCCCACCAGATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTaa                                                                                                                                >  1:767557/1‑140 (MQ=255)  aTTGGCAACGGCATCAATTGCGGACGGACGCCCCACCAGATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTaa                                                                                                                                >  2:567171/1‑140 (MQ=255)    tGGCAACGGCATCAATTGCGGACGGACGCCCCACCAGATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAg                                                                                                                              >  1:296674/1‑140 (MQ=255)      gCAACGGCATCAATTGCGGACGGACGCCCCACCAGATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTg                                                                                                                            <  2:988652/140‑1 (MQ=255)         aCGGCATCAATTGCGGACGGACGCCCCACCAGATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAg                                                                                                                         >  1:363825/1‑140 (MQ=255)               tCAATTGCGGACGGACGCCCCACCAGATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTcgcg                                                                                                                   <  1:364263/140‑1 (MQ=255)                      cggacggaCGCCCCACCAGATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGt                                                                                                            >  2:388608/1‑140 (MQ=255)                      cggacggaCGCCCCACCAGATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGt                                                                                                            >  1:355108/1‑140 (MQ=255)                            gacgCCCCACCAGATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCg                                                                                                      >  1:109076/1‑140 (MQ=255)                              cgCCCCACCAGATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAg                                                                                                    <  2:363825/140‑1 (MQ=255)                               gCCCCACCAGATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGc                                                                                                   <  2:296674/140‑1 (MQ=255)                                        gATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCa                                                                                          <  1:580411/140‑1 (MQ=255)                                         aTTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCAt                                                                                         >  1:34966/1‑140 (MQ=255)                                           tGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTg                                                                                       >  1:191853/1‑140 (MQ=255)                                           tGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTg                                                                                       >  2:493272/1‑140 (MQ=255)                                             aGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGt                                                                                     <  2:716136/140‑1 (MQ=255)                                             aGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGt                                                                                     >  1:716136/1‑140 (MQ=255)                                                   tCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGtttt                                                                                            >  2:350445/1‑127 (MQ=255)                                                   tCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGtttt                                                                                            <  1:350445/127‑1 (MQ=255)                                                     gCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGAccacca                                                                             <  2:355108/140‑1 (MQ=255)                                                         aaCTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACg                                                                         >  1:457172/1‑140 (MQ=255)                                                              cAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGa                                                                    >  2:722001/1‑140 (MQ=255)                                                                     atatCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCaa                                                             >  2:990552/1‑140 (MQ=255)                                                                      tatCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCaaa                                                            >  1:244575/1‑140 (MQ=255)                                                                      tatCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCaaa                                                            >  1:500609/1‑140 (MQ=255)                                                                        tCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATc                                                          <  1:591353/140‑1 (MQ=255)                                                                           ccACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGt                                                       <  1:644899/140‑1 (MQ=255)                                                                             aCTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGc                                                     <  2:767557/140‑1 (MQ=255)                                                                             aCTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGc                                                     <  1:990552/140‑1 (MQ=255)                                                                               tGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCt                                                   >  2:840798/1‑140 (MQ=255)                                                                               tGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCt                                                   >  2:472660/1‑140 (MQ=255)                                                                               tGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGCTTTCCGACCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCt                                                   >  2:781741/1‑140 (MQ=255)                                                                                ggCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCtt                                                  <  2:442310/140‑1 (MQ=255)                                                                                    aCCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGt                                              >  1:1023629/1‑140 (MQ=255)                                                                                        gTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGAc                                          <  1:166238/140‑1 (MQ=255)                                                                                            gACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGCCCGCCACACCCTCGGCGCGCAAATGAGCTGCTTTCGTGGACAGCg                                      <  2:823906/140‑1 (MQ=255)                                                                                            gACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCg                                      <  1:567171/140‑1 (MQ=255)                                                                                               cccGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAg                                   <  2:156083/140‑1 (MQ=255)                                                                                                ccGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGt                                  >  1:184534/1‑140 (MQ=255)                                                                                                    cACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCg                              <  2:203555/140‑1 (MQ=255)                                                                                                    cACGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCg                              <  2:244575/140‑1 (MQ=255)                                                                                                      cGGTTCAGGCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGAc                            <  1:472660/140‑1 (MQ=255)                                                                                                             ggCGCGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGa                     <  1:781741/140‑1 (MQ=255)                                                                                                              gcgcGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGAc                    >  1:925092/1‑140 (MQ=255)                                                                                                              gcgcGAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGAc                    <  2:34966/140‑1 (MQ=255)                                                                                                                 cgAGATAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCaa                 >  2:461132/1‑140 (MQ=255)                                                                                                                   agaTAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGAc                    >  1:800498/1‑135 (MQ=255)                                                                                                                   agaTAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGAc                    <  1:675015/135‑1 (MQ=255)                                                                                                                   agaTAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGAc                    >  2:675015/1‑135 (MQ=255)                                                                                                                   agaTAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGAc                    <  2:800498/135‑1 (MQ=255)                                                                                                                   agaTAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATc               >  1:86028/1‑140 (MQ=255)                                                                                                                   agaTAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATc               >  2:812992/1‑140 (MQ=255)                                                                                                                   agaTAACAACGTCATAGCGCTCCTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGAc                    >  2:710972/1‑135 (MQ=255)                                                                                                                   agaTAACAACGTCATAGCGCTCCTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGAc                    <  1:710972/135‑1 (MQ=255)                                                                                                                     aTAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATCAc             <  2:191853/140‑1 (MQ=255)                                                                                                                     aTAACAACGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATCAc             <  2:86028/140‑1 (MQ=255)                                                                                                                        acaacGTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATCACCAg          >  1:1010937/1‑140 (MQ=255)                                                                                                                             gTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATCACCAGTTgcg     <  1:461132/140‑1 (MQ=255)                                                                                                                             gTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATCACCAGTTgcg     <  2:457172/140‑1 (MQ=255)                                                                                                                             gTCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATCACCAGTTgcg     <  1:90278/140‑1 (MQ=255)                                                                                                                              tCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATCAc             >  2:149871/1‑131 (MQ=255)                                                                                                                              tCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATCAc             <  1:149871/131‑1 (MQ=255)                                                                                                                              tCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATCACCAGTTgcgc    >  2:733597/1‑140 (MQ=255)                                                                                                                              tCATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATCACCAGTTgcgc    >  1:591130/1‑140 (MQ=255)                                                                                                                               cATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATCACCAGTTGCGCa   >  2:814990/1‑140 (MQ=255)                                                                                                                               cATAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATCACCAGTTGCGCa   <  1:203960/140‑1 (MQ=255)                                                                                                                                aTAGCGCTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATCACCAGTTGCGCAt  <  2:894986/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      CATTGGCAACGGCATCAATTGCGGACGGACGCCCCACCAGATTGAGGATATCGCCGAACTGCAGGCTGATATCGCCACTGGCGACCAGTTCGACCCCGGCACGGTTCAGGCGCGAGATAACAACGTCATAGCGTTCTTTAAAGTGCAGGTCGCGAATACGTTTTCCGAGCACGTTTTCATTGGTGACCACCACACGCTCGACGCGCAAATCAGTGCCTTTCGTGGACAGCGAAGTATCGACCTCCTGACCAATCACCAGTTGCGCAT  >  NC_000913/3865202‑3865468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑