Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,333,372	A→G	H193H (CAT→CAC)	basR ←	DNA‑binding transcriptional dual regulator BasR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,333,372	0	A	G	100.0%	211.6 / NA	58	H193H (CAT→CAC)	basR	DNA‑binding transcriptional dual regulator BasR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/30);  total (28/30)

CGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGACAGCAGAGCATATTCTTTGGGCGTCA  >  NC_000913/4333235‑4333502                                                                                                                                          |                                                                                                                                   cGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGAtgtg                                                                                                                                  <  2:756829/140‑1 (MQ=255)   ttGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCa                                                                                                                                <  2:968287/140‑1 (MQ=255)    tGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCAc                                                                                                                               <  1:247898/140‑1 (MQ=255)    tGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCAc                                                                                                                               >  2:753135/1‑140 (MQ=255)    tGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCAc                                                                                                                               >  2:392242/1‑140 (MQ=255)    tGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCAc                                                                                                                               <  1:479429/140‑1 (MQ=255)         cAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCa                                                                                                                          <  2:528062/140‑1 (MQ=255)          aGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCAc                                                                                                                               <  2:395300/134‑1 (MQ=255)          aGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCAc                                                                                                                               >  1:395300/1‑134 (MQ=255)                    gTCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTc                                                                                                               >  2:742578/1‑140 (MQ=255)                    gTCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTc                                                                                                               >  2:225874/1‑140 (MQ=255)                     tCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCg                                                                                                              >  1:313274/1‑140 (MQ=255)                         cgcAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAggg                                                                                                          >  2:883676/1‑140 (MQ=255)                         cgcAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAggg                                                                                                          >  2:324736/1‑140 (MQ=255)                         cgcAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAggg                                                                                                          >  2:1017764/1‑140 (MQ=255)                                 aTGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGt                                                                                                  <  1:126584/140‑1 (MQ=255)                                  tGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTc                                                                                                 <  1:324736/140‑1 (MQ=255)                                  tGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTc                                                                                                 <  2:700119/140‑1 (MQ=255)                                  tGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTc                                                                                                 <  2:715858/140‑1 (MQ=255)                                  tGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTc                                                                                                 <  1:1017764/140‑1 (MQ=255)                                   gCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCCCTTCCAGGGTGTTGGTCGAGGGTTCATTGTcc                                                                                                <  2:878417/140‑1 (MQ=255)                                   gCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTcc                                                                                                >  1:565747/1‑140 (MQ=255)                                   gCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTcc                                                                                                <  1:950012/140‑1 (MQ=255)                                   gCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTcc                                                                                                <  2:313274/140‑1 (MQ=255)                                   gCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTcc                                                                                                <  1:594827/140‑1 (MQ=255)                                   gCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTcc                                                                                                <  1:753135/140‑1 (MQ=255)                                   gCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTcc                                                                                                <  2:762777/140‑1 (MQ=255)                                   gCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTcc                                                                                                <  1:742578/140‑1 (MQ=255)                                          aTTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTtat                                                                                         >  1:292730/1‑140 (MQ=255)                                             cAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGa                                                                                      <  1:883676/140‑1 (MQ=255)                                               aTTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATg                                                                                    <  2:905356/140‑1 (MQ=255)                                                    ttttCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGtt                                                                               >  1:923188/1‑140 (MQ=255)                                                    ttttCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGtt                                                                               >  2:862990/1‑140 (MQ=255)                                                      ttCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGt                                                                             >  2:769642/1‑140 (MQ=255)                                                      ttCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGt                                                                             >  1:986932/1‑140 (MQ=255)                                                       tCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTa                                                                            <  2:565747/140‑1 (MQ=255)                                                             ttCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTAATTGTCCCAGTTATAGATGTCGTTGTAGAGAAt                                                                      >  2:380062/1‑140 (MQ=255)                                                                   aCCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCg                                                                <  1:774879/140‑1 (MQ=255)                                                                        cATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCa                                                           >  1:302703/1‑140 (MQ=255)                                                                         atatAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCAc                                                          <  2:986932/140‑1 (MQ=255)                                                                              gCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGAc                                                     >  1:276860/1‑140 (MQ=255)                                                                                 aaaGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGActgc                                                  <  1:392242/140‑1 (MQ=255)                                                                                  aaGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGActgcc                                                 >  2:302494/1‑140 (MQ=255)                                                                                  aaGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGActgc                                                  >  1:585189/1‑139 (MQ=255)                                                                                  aaGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGActgc                                                  <  2:585189/139‑1 (MQ=255)                                                                                   aGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGActgcct                                                >  1:538077/1‑140 (MQ=255)                                                                                    gCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGActgcctg                                               <  2:302703/140‑1 (MQ=255)                                                                                         gcACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTg                                          <  2:276860/140‑1 (MQ=255)                                                                                             ggTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCa                                      <  1:862990/140‑1 (MQ=255)                                                                                             ggTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCa                                      <  1:769642/140‑1 (MQ=255)                                                                                                         ggCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCAtt                                    >  2:194983/1‑130 (MQ=255)                                                                                                         ggCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCAtt                                    <  1:194983/130‑1 (MQ=255)                                                                                                         ggCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGAca                          >  2:507803/1‑140 (MQ=255)                                                                                                         ggCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGAca                          >  1:264880/1‑140 (MQ=255)                                                                                                         ggCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGAca                          >  2:259618/1‑140 (MQ=255)                                                                                                         ggCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGAca                          >  2:45058/1‑140 (MQ=255)                                                                                                          gCTTTGCCCACTTTGTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGAcag                         <  2:292730/140‑1 (MQ=255)                                                                                                                        gTCGCGCAGATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGACAGCAGAGCATATTCtt           >  1:442957/1‑140 (MQ=255)                                                                                                                                gATTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGACAGCAGAGCATATTCTTTGGGCGTc   <  1:302494/140‑1 (MQ=255)                                                                                                                                 aTTGTGGATGTGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGACAGCAGAGCATATTCTTTGGGCGTCa  >  2:522590/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                   CGTTGGCGCAGCGATATTGGTCGGCGCAGAAAATGCATCAGATTCAATTAGTTTTCCTCATTCGCGACCAGCATATAGCCAAAGCCGCGCACGGTGCGGATACGGGCTTTGCCCACTTTGTCGCGCAGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGACAGCAGAGCATATTCTTTGGGCGTCA  >  NC_000913/4333235‑4333502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑